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2011 Fiscal Year Final Research Report

Development of a DNA marker for detecting tolerance to cold-induced seed coat discoloration in soybean

Research Project

  • PDF
Project/Area Number 20380001
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Breeding science
Research InstitutionHirosaki University

Principal Investigator

SENDA Mineo  弘前大学, 農学生命科学部, 准教授 (30261457)

Co-Investigator(Kenkyū-buntansha) SANO Teruo  弘前大学, 農学生命科学部, 教授 (30142699)
Project Period (FY) 2008 – 2011
Keywords抵抗性 / 耐性
Research Abstract

In yellow soybean, seed coat discoloration occurs when plants are exposed to low temperature after the onset of flowering, a phenomenon named cold-induced discoloration abbreviated as CD. CD reduces the commercial value of yellow soybean, and therefore DNA markers are required tocreate CD-tolerant cultivars. However, it was not even known how low temperature causes discoloration in the seed coat. In this study, we investigated the occurrence and tolerance mechanisms of CD. Furthermore, based on the findings, we developed a DNA marker being effective in selecting the CD-tolerant plants.

  • Research Products

    (20 results)

All 2012 2011 2010 2009 2008 Other

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (12 results) Book (2 results) Remarks (1 results)

  • [Journal Article] Endogenous RNA interference of chalcone synthase genes in soybean : Formation of double-stranded RNA of GmIRCHS transcripts and structure of the 5'and 3'ends of sh ort interfering RNAs2011

    • Author(s)
      Kurauchi, T., A. Kasai, M. Tougou and M. Senda
    • Journal Title

      Journal of Plant Physiology

      Volume: 168 Pages: 1264-1270

    • Peer Reviewed
  • [Journal Article] Variation of GmIRCHS (Glycine max inverted-repeat CHS pseudogene) is related to tolerance of low temperature-induced seed coat discoloration in yellow soybean2011

    • Author(s)
      Ohnishi, S., H. Funatsuki, A. Kasai, T. Kurauchi, N. Yamaguchi, T. Takeuchi, H. Yamazaki, H. Kurosaki, S. Shirai, T. Miyoshi, H. Horita and M. Senda
    • Journal Title

      Theoretical and Applied Genetics

      Volume: 122 Pages: 633-642

    • Peer Reviewed
  • [Journal Article] Endogenous short interfering RNAs of chalcone synthase genes associated with inhibition of seed coat pigmentation in soybean2009

    • Author(s)
      Kurauchi, T., T. Matsumoto, A. Taneda, T. SanoandM. Senda
    • Journal Title

      Breeding Science

      Volume: 59 Pages: 419-426

    • Peer Reviewed
  • [Journal Article] Molecular mechanism of seed coat discoloration induced by low temperature in yellow soybean2009

    • Author(s)
      Kasai, A., S. Ohnishi, H. Yamazaki, H. Funatsuki, T. Kurauchi, T. Matsumoto, S. Yumoto and M. Senda
    • Journal Title

      Plant and Cell Physiology

      Volume: 50 Pages: 1090-1098

    • Peer Reviewed
  • [Journal Article] A novel major quantitative trait locus controlling seed development at low temperature in soybean (Glycine max)2009

    • Author(s)
      Ikeda, T., S. Ohnishi, M. Senda , T. Miyoshi, M. Ishimoto, K. Kitamura and H. Funatsuki
    • Journal Title

      heoretical and Applied Genetics

      Volume: 118 Pages: 1477-1488

    • Peer Reviewed
  • [Presentation] 着色ダイズにおける種皮着色過程の解析2012

    • Author(s)
      松澤めぐみ・西村さつき・乗田理恵・川崎通夫・千田峰生
    • Organizer
      日本育種学会
    • Place of Presentation
      宇都宮大学(栃木県宇都宮市)
    • Year and Date
      2012-03-30
  • [Presentation] 黄ダイズ品種フクユタカの臍着色形質に関する解析2012

    • Author(s)
      佐野麻衣子・松澤めぐみ・森あゆみ・穴井豊昭・川崎通夫・千田峰生
    • Organizer
      日本育種学会
    • Place of Presentation
      宇都宮大学(栃木県宇都宮市)
    • Year and Date
      2012-03-30
  • [Presentation] 黄ダイズの種皮着色抑制に関与する内在性siRNAの解析2011

    • Author(s)
      千田峰生・種田晃人
    • Organizer
      ミヤコグサ・ダイズシンポジウム
    • Place of Presentation
      かずさアカデミアホール(千葉県木更津市)
    • Year and Date
      2011-05-27
  • [Presentation] 黄ダイズの種皮着色抑制と種皮着色現象2011

    • Author(s)
      千田峰生
    • Organizer
      ダイズ研究会
    • Place of Presentation
      北海道大学(北海道札幌市)
    • Year and Date
      2011-03-05
  • [Presentation] ダイズ種皮着色抑制に関与するCHS遺伝子由来の内在性siRNAsに関する研究I.ノーザンブロット分析を用いた解析2010

    • Author(s)
      松本拓郎・千田峰生
    • Organizer
      日本育種学会
    • Place of Presentation
      京都大学(京都市)
    • Year and Date
      2010-03-27
  • [Presentation] ダイズ種皮着色抑制に関与するCHS遺伝子由来の内在性siRNAsに関する研究II.次世代シーケンサーを用いた解析2010

    • Author(s)
      倉内佑・種田晃人・佐野輝男・千田峰生
    • Organizer
      日本育種学会
    • Place of Presentation
      京都大学(京都市)
    • Year and Date
      2010-03-27
  • [Presentation] 低温着色感受性および抵抗性ダイズ品種間に見出されたGmIRCHS領域の構造変異2009

    • Author(s)
      葛西厚史・大西志全・船附秀行・千田峰生
    • Organizer
      日本育種学会
    • Place of Presentation
      北海道大学(北海道札幌市)
    • Year and Date
      2009-09-26
  • [Presentation] ダイズGmIRCHS領域の構造差異は低温着色抵抗性と強く関連づけられ選抜DNAマーカーとして有用である2009

    • Author(s)
      大西志全・千田峰生・葛西厚史・船附秀行・山崎敬之・山口直矢・三好智明
    • Organizer
      日本育種学会
    • Place of Presentation
      北海道大学(北海道札幌市)
    • Year and Date
      2009-09-26
  • [Presentation] ダイズI遺伝子座近傍のゲノム構造変異と耐冷性QTL2009

    • Author(s)
      船附秀行・池田達哉・千田峰生・大西志全・夏正俊・原田久也・中本有美・小松邦彦・石本政男・喜多村啓介
    • Organizer
      日本育種学会
    • Place of Presentation
      北海道大学(北海道札幌市)
    • Year and Date
      2009-09-26
  • [Presentation] RNAサイレンシングと有用形質2009

    • Author(s)
      千田峰生
    • Organizer
      日本育種学会シンポジウム
    • Place of Presentation
      北海道大学(北海道札幌市)
    • Year and Date
      2009-09-25
  • [Presentation] ダイズにおける低温着色粒発生の分子メカニズムの解明2008

    • Author(s)
      葛西厚史・大西志全・山崎敬之・船附秀行・倉内佑・松本拓郎・湯本節三・白井滋久・三好智明・千田峰生
    • Organizer
      日本育種学会
    • Place of Presentation
      滋賀県立大学(滋賀県彦根市)
    • Year and Date
      2008-10-11
  • [Presentation] ダイズにおける新規耐冷性QTLの同定2008

    • Author(s)
      池田達哉・大西志全・千田峰生・三好智明・石本政男・喜多村啓介・船附秀行
    • Organizer
      日本育種学会
    • Place of Presentation
      滋賀県立大学(滋賀県彦根市)
    • Year and Date
      2008-10-11
  • [Book] 植物の分子育種学2011

    • Author(s)
      千田峰生, 他
    • Total Pages
      156-166
    • Publisher
      講談社
  • [Book] 種子の科学とバイオテクノロジー2009

    • Author(s)
      千田峰生, 他
    • Total Pages
      131-134
    • Publisher
      学会出版センター
  • [Remarks]

    • URL

      http://nature.cc.hirosaki-u.ac.jp/gene/senda/senda.html

URL: 

Published: 2013-07-31  

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