Analysis of molecular mechanism, origin and diversity of gametophytic self-incompatibility

2010 Fiscal Year Self-evaluation Report

• Project/Area Number: 20380003
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Breeding science
• Research Institution: Chiba University
• Principal Investigator: SASSA Hidenori Chiba University, 大学院・園芸学研究科, 准教授 (50295507)
• Project Period (FY): 2008 – 2011
• Keywords: 自家不和合性 / 雌ずい / 花粉 / RNase / バラ科 / ナス科

Research Abstract

自家不和合性は同じ個体あるいは遺伝的に近縁な個体からの花粉を雌ずいが認識・拒絶する機構であり、免疫を持たない植物における自他識別機構として古くから注目を集めてきた。また、その機構解明は、画期的植物品種改良(育種)技術や採種・栽培技術の開発にも直結する。最も多くの植物種に見られる機構である配偶体型自家不和合性は、雌ずいと花粉双方の認識特異性決定遺伝子が同定されたが、それらの細胞内での機能や他の因子との相互作用などは未解明のままである。
本研究では、更にバラ科と類似した機構を持つナス科植物も対象に加え、遺伝分析、形質転換実験、タンパク質相互作用解析など様々なアプローチを駆使し、得られた知見をバラ科・ナス科間で相互に比較し、起源・進化の観点も含めて配偶体型自家不和合性機構の総合的解明を目指す。

Research Products

• [Journal Article] Apple S locus region represents a large cluster of related, polymorphic and pollen-specific F-box genes. (2010)
  • Author(s): Minamikawa, M., Kakui, H., Wang, S., Kotoda, N., Kikuchi, S., Koba, T., Sassa, H.
  • Journal Title: Plant Mol Biol 74 Pages: 143-154
  • Peer Reviewed
• [Journal Article] Pollen-expressed F-box gene family and mechanism of S-RNase-based gametophytic self-incompatibility (GSI) in Rosaceae. (2010)
  • Author(s): Sassa, H., Kakui, H., Minamikawa, M
  • Journal Title: Sex.Plant Reprod. 23 Pages: 39-43
  • Peer Reviewed
• [Journal Article] Identification and functional analysis of pistil-part self-incompatibility factor HT-B of Petunia. (2009)
  • Author(s): Puerta, A.R., Ushijima, K., Koba, T., Sassa, H.
  • Journal Title: J Exp Bot 60 Pages: 1309-1318
  • Peer Reviewed
• [Journal Article] Pistil non-S-specific self-incompatibility factor HT-B is tightly linked to the HT-like gene HTL in Petunia. (2009)
  • Author(s): Puerta, A.R., Takagawa, M., Sassa, H.
  • Journal Title: Breed Sci 59 Pages: 99-101
  • Peer Reviewed
• [Journal Article] Determination of partial genomic sequences and development of a CAPS system of the S-RNase gene for the identification of 22 S haplotypes of apple (Malus × domestica Borkh.). (2009)
  • Author(s): Kim, H., Kakui, H., Kotoda, N., Hirata, Y., Koba, T., Sassa, H.
  • Journal Title: Mol Breed 23 Pages: 463-472
  • Peer Reviewed
• [Presentation] S-RNase and SFBB family F-box genes are located in sub-teromeric and heterochromatic chromosomal region in apple. (2010)
  • Author(s): Wang, S., Kakui, H., Kikuchi, S., Koba, T., Sassa H.
  • Organizer: XXI International Congress on Sexual Plant Reproduction.PSR6.22, Abstract
  • Place of Presentation: Bristol, UK
  • Year and Date: 20100000
• [Presentation] Characterization of the F-box gene cluster linked to the S locus of apple. (2010)
  • Author(s): Minamikawa, M., Kakui, H., Wang, S., Kotoda, N., Kikuchi, S., Koba, T., Sassa, H.
  • Organizer: XXI International Congress on Sexual Plant Reproduction PSR6.23, Abstract
  • Place of Presentation: Bristol, UK
  • Year and Date: 20100000
• [Presentation] Analysis of pollen-part S specificity of S4sm, a stylar-part self-compatible S haplotype in Japanese pear. (2010)
  • Author(s): Kakui, H., Sassa H., Kitaguchi, M., Kato, S., Kato , M.
  • Organizer: XXI International Congress on Sexual Plant Reproduction PSR6.25, Abstract
  • Place of Presentation: Bristol, UK
  • Year and Date: 20100000
• [Presentation] Characterization of multiple F-box genes linked to the S locus of apple and Japanese pear. (2008)
  • Author(s): Sassa, H., Kakui, H., Minamikawa, M.
  • Organizer: Frontiers of Sexual Plant Reproduction III, Abstract
  • Place of Presentation: Tucson, USA(招待講演)
  • Year and Date: 20080000