Analysis of molecular mechanism, origin and diversity of gametophytic self-incompatibility systems

2011 Fiscal Year Final Research Report

• Project/Area Number: 20380003
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Breeding science
• Research Institution: Chiba University
• Principal Investigator: SASSA Hidenori 千葉大学, 大学院・園芸学研究科, 准教授 (50295507)
• Co-Investigator(Kenkyū-buntansha): USHIJIMA Koichiro 岡山大学, 大学院・自然科学研究科, 助教 (20379720)
• Co-Investigator(Renkei-kenkyūsha): KOTODA Nobuhiro (独)農業・食品産業技術研究機構, 果樹研究所, 主任研究員 (50355426)
• Project Period (FY): 2008 – 2011
• Keywords: 遺伝子 / タンパク質

Research Abstract

Analysis of candidates for pollen-determinant in Japanese pear and apple of Rosaceae suggested that multiple and related F-box genes, SFBB, are collaboratively detoxify non-self pistil determinant, S-RNase. Isolation and functional analysis of HT-B gene, a non-S-specific self-incompatibility factor, of petunia showed that, unlike other solanaceous species, even a dramatic suppression of HT-B transcript resulted in partial breakdown of self-incompatibility in petunia. This may suggest slight functional divergence of HT-B gene in different species of Solanaceae.

Research Products

• [Journal Article] Sequence divergence and loss-of-function phenotypes of S locus F-box brothers (SFBB) genes are consistent with non-self recognition by multiple pollen determinants in self-incompatibility of Japanese pear (Pyrus pyrifolia) (2011)
  • Author(s): Kakui, H., Kato, M., Ushijima, K., Kitaguchi, M., Kato, S. Sassa, H.
  • Journal Title: Plant J. Volume: 68 Pages: 1028-1038
  • Peer Reviewed
• [Journal Article] Apple S locus region represents a large cluster of related, polymorphic and pollen-specific F-box genes (2010)
  • Author(s): Minamikawa, M., Kakui, H., Wang, S., Kotoda, N., Kikuchi, S., Koba, T. Sassa, H.
  • Journal Title: Plant Mol.Biol. Volume: 74 Pages: 143-154
  • Peer Reviewed
• [Journal Article] Identification and functional analysis of pistil-partself-incompatibility factor HT-B ofPetunia (2009)
  • Author(s): Puerta, A. R., Ushijima, K., Koba, T. Sassa, H.
  • Journal Title: J. Exp. Bot. Volume: 60 Pages: 1309-1318
  • Peer Reviewed
• [Journal Article] Pistil non-S-specificself-incompatibility factor HT-B is tightly linked to the HT-like gene HTL in Petunia (2009)
  • Author(s): Puerta, A. R., Takagawa, M. Sassa, H.
  • Journal Title: Breed. Sci Volume: 59 Pages: 99-101
  • Peer Reviewed
• [Journal Article] Determination of partial genomic sequences and development of a CAPS system of the S-RNase gene for the identification of 22 Shaplotypes of apple (2009)
  • Author(s): Kim, H., Kakui, H., Kotoda, N., Hirata, Y., Koba, T. Sassa, H.
  • Journal Title: Mol. Breed Volume: 23 Pages: 463-472
  • Peer Reviewed
• [Journal Article] Identification of self-incompatibility related proteins in the pistil of Japanes epear (Pyrus pyrifolia (Burm. f.)) by proteome analysis
  • Author(s): Uchida, A., Sassa, H., Takenaka, S., Sakakibara, Y., Suiko, M. Kunitake, H.
  • Journal Title: Plant Omics Volume: (in press)
  • Peer Reviewed
• [Presentation] Non-self recognition by multiple F-box genes inself-incompatibility of Japanese pear (Pyrus pyrifolia) (2012)
  • Author(s): Sassa, H., Kakui, H., Kato, M. Ushijima, K.
  • Organizer: Plant and AnimalGenome XX
  • Place of Presentation: San Diego, CA, USA
  • Year and Date: 20120100
• [Presentation] Structural features of the S locus of apple (2011)
  • Author(s): Sassa H., Wang, S., Minamikawa, M., Kakui, H., Kikuchi, S. Koba, T.
  • Organizer: Floral Biology and S-incompatibility in fruit species
  • Place of Presentation: San Michele all'Adige, Italy
  • Year and Date: 20110600
• [Presentation] ニホンナシ品種「なつひかり」の弱い自家不和合性の解析 (2011)
  • Author(s): 加藤雅樹, 加藤修, 北口美代子, 佐々英徳
  • Organizer: 日本育種学会第120回講演会
  • Place of Presentation: 福井県立大学
  • Year and Date: 20110000
• [Presentation] S-RNase and SFBB family F-box genes are located in sub-teromeric and heterochromatic chromosomal region in apple (2010)
  • Author(s): Wang, S., Kakui, H., Kikuchi, S., Koba,T. Sassa H.
  • Organizer: XXI International Congress on Sexual Plant Reproduction
  • Place of Presentation: Bristol, UK
  • Year and Date: 20100000
• [Presentation] Characterization of the F-box gene cluster linked to the S locus of apple (2010)
  • Author(s): Minamikawa, M., Kakui, H., Wang, S., Kotoda, N., Kikuchi, S., Koba, T. Sassa, H.
  • Organizer: XXI International Congress on Sexual Plant Reproduction
  • Place of Presentation: Bristol, UK
  • Year and Date: 20100000
• [Presentation] Analysis of pollen-part S specificity of S4sm, a stylar-part self-compatible S haplotype in Japanese pear (2010)
  • Author(s): Kakui, H., Sassa H., Kitaguchi, M., Kato, S. Kato , M.
  • Organizer: XXI International Congress on Sexual Plant Reproduction
  • Place of Presentation: Bristol, UK
  • Year and Date: 20100000
• [Presentation] リンゴにおける自家不和合性関連遺伝子SBP1 (S-RNase binding protein1)ホモログの同定と解析 (2010)
  • Author(s): 藤井大輔, 角井宏行, 古藤田信博, 牛島幸一郎, 佐々英徳
  • Organizer: 日本育種学会第118回講演会
  • Place of Presentation: 秋田県立大学
  • Year and Date: 20100000
• [Presentation] ニホンナシにおける雌ずい側自家和合性変異ハプロタイプS4smの花粉側特異性の解析 (2010)
  • Author(s): 角井宏行, 加藤雅樹, 加藤修, 北口美代子, 佐々英徳
  • Organizer: 日本育種学会第118回講演会
  • Place of Presentation: 秋田県立大学
  • Year and Date: 20100000
• [Presentation] Identification and functional analysis of non-S-specific pistil-part self-incompatibility factor HT-B of Petunia (2009)
  • Author(s): Puerta AR, Ushijima K, Koba T, Sassa H.
  • Organizer: 日本育種学会第115回講演会
  • Place of Presentation: つくば国際会議場
  • Year and Date: 20090000
• [Presentation] リンゴS遺伝子座に連鎖するF-box遺伝子群の解析 (2009)
  • Author(s): 南川舞, 角井宏行, 古藤田信博, 佐々英徳
  • Organizer: 日本育種学会第116回講演会
  • Place of Presentation: 北海道大学
  • Year and Date: 20090000
• [Presentation] S-RNase遺伝子のCAPS分析によるリンゴ品種のS対立遺伝子22種類の識別 (2008)
  • Author(s): Kim HT, 角井宏行, 古藤田信博, 木庭卓人, 佐々英徳
  • Organizer: 日本育種学会第114回講演会
  • Place of Presentation: 滋賀県立大学
  • Year and Date: 20080000
• [Presentation] Characterization of multiple F-box genes linked to the S locus of apple and Japanese pear (2008)
  • Author(s): Sassa, H., Kakui, H., Minamikawa, M.
  • Organizer: Frontiers of Sexual Plant Reproduction III
  • Place of Presentation: Tucson, USA
  • Year and Date: 20080000