2011 Fiscal Year Final Research Report
Production of barley chromosome dissection lines anddevelopment of PCR-based screening method
| Project/Area Number |
20380006
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Breeding science
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| Research Institution | Kyoto University |
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Principal Investigator |
ENDO Takashi 京都大学, 農学研究科, 教授 (60068830)
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| Project Period (FY) |
2008 – 2011
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| Keywords | オオムギ / コムギ / 染色体 / 染色体地図 / 染色体断片 / Gcシステム / PCR |
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| Research Abstract |
It was aimed to produce dissection lines of barley chromosomes(1H~ 7H) in commonwheat using the gametocidal(Gc) system and to develop a PCR-based method for theselection of the dissection lines. As a result, more than 50 dissection lines wereobtained for 2H, 3H, 4H, 5H and 7H, and effective PCR markers were found to detectthe presence of the individual dissected barley segments. Enough number ofdissection lines for 6H were not obtained and it was found difficult to apply the Gcsystem to 1H.
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