Development of DNA markers and QTL mapping relating to oleic acid contents in peanut.

2010 Fiscal Year Final Research Report

• Project/Area Number: 20380008
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Breeding science
• Research Institution: Kazusa DNA Research Institute
• Principal Investigator: TABATA Satoshi Kazusa DNA Research Institute, 副所長 (70197549)
• Co-Investigator(Kenkyū-buntansha): ISOBE Sachiko (財)かずさDNA研究所, 植物ゲノム研究部, 主任研究員 (20343973) ; AOKI Koh (財)かずさDNA研究所, 生体機能応用研究室, 室長 (30344021)
• Project Period (FY): 2008 – 2010
• Keywords: 植物 / 育種 / ゲノム / 遺伝学 / 脂質

Research Abstract

In order to accelerate breeding for high oleic acid contents in peanut, two selection markers were developed from candidate gene sequences controlling oleic acid contents. In addition, genome wide polymorphic DNA markers (microsatellite and transposon element markers) were developed and mapped on genetic linkage maps of peanut. Those genome wide DNA markers as well as the two candidate gene markers were used for marker assisted selection of the high-oleic peanut breeding program. As a result, we succeed to reduce breeding periods from five to three years.

Research Products

• [Journal Article] Large-scale development of EST-derived SSR markers and diversity analysis in Arachis spp. (2011)
  • Author(s): Koilkonda P, Sato S, Satoshi Tabata, Shirasawa K, Hirakawa H, Sakai H, Sasamoto S, Watanabe A, Wada T, Kishida Y, Tsuruoka H, Fujishiro T, Yamada M, Kohara M, Suzuki S, Hasegawa M, Kiyoshima H, Isobe S
  • Journal Title: Molecular Breeding Pages: DOI : 10.1007/s11032-011-9604-8
  • Peer Reviewed
• [Presentation] ラッカセイに見出したMITE型トランスポゾンの特徴 (2010)
  • Author(s): 白澤健太,Koilkonda P,田畑哲之,磯部祥子
  • Organizer: 育種学研究(12(別2):282)
  • Year and Date: 20100000
• [Presentation] Marker assisted backcrossing selection (MABS) for oleic acid content in peanut. (2009)
  • Author(s): Shirasawa K, Koilkonda P, Naito Y, Watanabe M, Suzuki S, Hasegawa H, Kiyoshima H, Kuwata C, Tabata S, Isobe S
  • Organizer: International Plant Molecular Breeding Congress.
  • Place of Presentation: USA
  • Year and Date: 20091025-20091101
• [Presentation] Development of microsatellite, single nucleotide polymorphism, and transposon markers for peanut breeding. (2009)
  • Author(s): Shirasawa K, Koilkonda P, Naito Y, Watanabe M, Suzuki S, Hasegawa H, Kiyoshima H, Kuwata C, Tabata S, Isobe
  • Organizer: Advances in Arachis through Genomics & Biotechnology-2009
  • Place of Presentation: Bamako, Mali
  • Year and Date: 20091019-20091022
• [Presentation] Development of microsatellite, SNP and AFLP markers in cultivated peanut. (2008)
  • Author(s): Isobe S, Sato S, Shirasawa K, Suzuki S, Watanabe M, Tabata S.
  • Organizer: Third international conference of the Peanut Research Community on Advances in Arachis through Genomics and Biotechnology (AAGB-2008), ICRISAT
  • Place of Presentation: Hederabad India
  • Year and Date: 20081104-20081108
• [Remarks] ホームページ等
• [Remarks] 落花生のDNAマーカーに関するデータベース
  • URL: http://marker.kazusa.or.jp/peanut