Studies on plant factors required for nonhost resistance against anthracnose fungi

2011 Fiscal Year Final Research Report

• Project/Area Number: 20380027
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant pathology
• Research Institution: Kyoto University
• Principal Investigator: TAKANO Yoshitaka 京都大学, 大学院・農学研究科, 准教授 (80293918)
• Project Period (FY): 2008 – 2011
• Keywords: 病害抵抗性 / 非宿主抵抗性

Research Abstract

The Arabidopsis lic1 mutants developed lesions by non-adapted pathogens, and the gene responsible for the mutants encodes a MACPF domain protein with unknown function. In this study, we showed that lesion development in the lic1 mutants is not due to pathogen entry, indicating that LIC1 is required for regulation of cell death in nonhost defense, and that PEN2-dependent synthesis of anti-microbial metabolite is involved in LIC1-mediated regulation for cell death. We also indicate that LIC1 is localized in plasma membrane, and functions together with LIC1 homologs. Furthermore, we found that EDR1 and GSH1 are involved in nonhost resistance.

Research Products

• [Journal Article] Arabidopsis ENHANCED DISEASE RESISTNANCE 1 is required for pathogen-induced expression of plant defensins in nonhost resistance, and acts through interference of MYC2-mediated repressor function (2011)
  • Author(s): Hiruma, K., Nishiuchi, T., Kato, T., Bednarek, P., Okuno, T., Schulze-Lefert, P., and Takano, Y.
  • Journal Title: Plant Journal Volume: 67 Pages: 980-992
  • DOI: DOI:10.1111/j.1365-313X.2011.04651.x
  • Peer Reviewed
• [Journal Article] Roles of EDR1 in non-host resistance (2011)
  • Author(s): Hiruma, K., and Takano, Y.
  • Journal Title: Plant Signaling & Behavior Volume: 6 Pages: 1831-1833
  • DOI: DOI:10.4161/psb.6.11.17494
  • Peer Reviewed
• [Journal Article] 植物病原性カビの新たな侵入戦略 (2011)
  • Author(s): 晝間敬、吉野香絵、高野義孝
  • Journal Title: 化学と生物 Volume: 49 Pages: 588-599
  • DOI: DOI:10.1271/kagakutoseibutsu.49.588
• [Journal Article] Entry mode-dependent function of an indole glucosinolate pathway in Arabidopsi for nonhost resistance a gainst anthracnose pathogens (2010)
  • Author(s): Hiruma, K., Onozawa-Komori, M., Takahashi, F., Asakura, M., Bednarek, P., Okuno, T., Schulze-Lefert, P., and Takano, Y
  • Journal Title: The Plant Cell Volume: 22 Pages: 2429-2443
  • URL: http://dx.doi.org/10.1105/tpc.110
  • Peer Reviewed
• [Journal Article] 植物病原性カビの感染戦略 (2010)
  • Author(s): 高野義孝
  • Journal Title: 生物の科学 遺伝 Volume: 64 Pages: 20-25
• [Journal Article] Atg26-mediated pexophagy is required for host invasion by the plant pathogenic fungus Colletotrichum orbiculare (2009)
  • Author(s): Asakura, M., Ninomiya, S., Sugimoto, M., Oku, M., Yamashita, S., Okuno, T., Sakai, S., and Takano, Y.
  • Journal Title: The Plant Cell Volume: 21 Pages: 1291-1304
  • URL: http://dx.doi.org/10.1105/tpc.108.060996
• [Presentation] 非宿主抵抗反応における細胞死制御に関与するシロイヌナズナのLIC1/NSL1の研究 (2012)
  • Author(s): 福永聡、十亀美穂、小野澤(小森)真理子、奥野哲郎、高野義孝
  • Organizer: 日本植物病理学会大会
  • Place of Presentation: 福岡国際会議場(福岡)
  • Year and Date: 2012-03-28
• [Presentation] グルタチオン生合成酵素GSH1は、クワ炭疽病菌に対する非宿主抵抗性に必要である (2011)
  • Author(s): 晝間敬、奥野哲郎、高野義孝
  • Organizer: 日本植物病理学会関西部会
  • Place of Presentation: サンポートホール高松(香川)
  • Year and Date: 2011-10-01
• [Presentation] シロイヌナズナの非宿主抵抗反応における抗菌タンパク質の分泌制御に関する研究 (2011)
  • Author(s): 渡邊智史、奥野哲郎、高野義孝
  • Organizer: 日本植物病理学会関西部会
  • Place of Presentation: サンポートホール高松(香川)
  • Year and Date: 2011-10-01
• [Presentation] 炭疽病菌に対するシロイヌナズナの非宿主抵抗反応におけるディフェンシンの分泌制御 (2011)
  • Author(s): 渡邊智史、晝間敬、奥野哲郎、高野義孝
  • Organizer: 平成23年度日本植物病理学会大会
  • Place of Presentation: 東京農工大(東京都)
  • Year and Date: 2011-03-27
• [Presentation] Arabidopsis EDR1 is required for pathogen-induced expression of plant defensins in nonhost resistance (2010)
  • Author(s): Hiruma, K., Nishiuchi, T., Kato, T., Okuno, T., and Takano, Y
  • Organizer: 21th International Conference on Arabidopsis Research
  • Place of Presentation: Pacifico Yokohama, Yokohama, Japan
  • Year and Date: 2010-06-06
• [Presentation] EDR1 positively regulates nonhost defense response independently of PEN2 (2009)
  • Author(s): Hiruma, K., Nishiuchi, T., Kato, T., Asano, T., Okuno, T., and Takano, Y
  • Organizer: International Congress of Molecular Plant-Microbe Interactions
  • Place of Presentation: Quebec, Canada
  • Year and Date: 20090719-23
• [Presentation] Atg26-enhanced pexophagy is required for host invasion by the plant pathogenic fungus Colletotrichum orbicular (2009)
  • Author(s): Asakura, M., Ninomiya, S., Sugimoto, M., Oku, M., Yamashita, S., Okuno, T., Sakai, Y and Takano, Y.
  • Organizer: nternational Congress of Molecular Plant-Microbe Interactions
  • Place of Presentation: Quebec, Canada
  • Year and Date: 20090719-23
• [Presentation] LIC1 is involved in cell death regulation during nonhost defense response of Arabidopsis thaliana (2009)
  • Author(s): Sogame, M., Onozawa, M., Saitoh, H., Terauchi, R., Okuno, T., and Takano, Y
  • Organizer: International Congress of Molecular Plant-Microbe Interactions
  • Place of Presentation: Quebec, Canada
  • Year and Date: 2009-07-19
• [Presentation] EDR1はPEN2に依存しない非宿主抵抗反応を正に制御している (2009)
  • Author(s): 晝間敬、西内巧、加藤智朗、浅野智哉、奥野哲郎、高野義孝
  • Organizer: 日本植物病理学会大会
  • Place of Presentation: 山形県生涯学習センター
  • Year and Date: 2009-03-27
• [Presentation] LIC1遺伝子は非宿主抵抗反応における細胞死制御に関与している (2009)
  • Author(s): 小野澤真理子、十亀美穂、斉藤宏昌、寺内良平、奥野哲郎、高野義孝
  • Organizer: 日本植物病理学会大会
  • Place of Presentation: 山形県生涯学習センター
  • Year and Date: 2009-03-27
• [Presentation] 炭疽病菌に対するシロイヌナズナの非宿主抵抗性におけるPEN2要求性の研究 (2008)
  • Author(s): 晝間敬、小野澤真理子、Volker Lipka、奥野哲郎、Paul Schulze-Lefert、高野義孝
  • Organizer: 日本植物病理学会関西部会
  • Place of Presentation: 和歌山ビッグ愛
  • Year and Date: 2008-09-26