2010 Fiscal Year Final Research Report
Functional analysis of glycolysis enzyme GAPDH in HR cell death
| Project/Area Number |
20380030
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plant pathology
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| Research Institution | National Institute of Agrobiological Sciences |
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Principal Investigator |
NISHIZAWA Yoko National Institute of Agrobiological Sciences, 植物・微生物間相互作用研究ユニット, 上級研究員 (40355756)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 植物免疫 / 過敏感細胞死 / MAMPs受容体 |
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| Research Abstract |
Various defense reactions such as hypersensitive cell death are induced in plants on recognition of microbe-associated molecular patterns (MAMPs). In this study, we showed that chitin oligosaccharides, which are representative fungal MAMPs, elicit the production of both reactive oxygen and nitrogen species, and the production of these radicals is required for the induction of hypersensitive cell death in rice cultured cells. In addition, we performed functional analysis of rice EL5 gene, whose expression is induced by chitin, confirmation of interaction between EL5 and GAPDH in rice cells, and characterization of GAPDH during cell death. Our data indicate that GAPDH, a glycolysis enzyme, is also involved in the induction of cell death in plants.
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