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2011 Fiscal Year Final Research Report

Studies on bacterial microaerobic metabolism and its regulation

Research Project

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Project/Area Number 20380046
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied microbiology
Research InstitutionThe University of Tokyo

Principal Investigator

ARAI Hiroyuki  東京大学, 大学院・農学生命科学研究科, 助教 (70291052)

Project Period (FY) 2008 – 2011
Keywords好気 / 嫌気 / 微好気 / 呼吸 / 光合成 / 脱窒 / 緑膿菌
Research Abstract

Microbial metabolisms specific to microaerobic conditions were investigated mainly utilizing DNA microarray. Pseudomonas aeruginosa, anoxygenic photosynthetic bacteria, and thermophilic hydrogen oxidizing bacterium were used as research materials. The regulatory mechanism of multiple energy metabolisms especially for terminal oxidases and the transcriptional regulators that mediate the regulation were clarified. We also found a novel functional diversification mechanism of high affinity terminal oxidase in P. aeruginosa.

  • Research Products

    (20 results)

All 2012 2011 2010 2009

All Journal Article (7 results) (of which Peer Reviewed: 6 results) Presentation (13 results)

  • [Journal Article] A novel enzymatic system against oxidative stress in the thermophilic hydrogen-oxidizing bacterium Hydrogenobacter thermophilus2012

    • Author(s)
      Yuya Sato, Masafumi Kameya, Shinya Fushinobu, Takayoshi Wakagi, Hiroyuki Arai, Masaharu Ishii, and Yasuo Igarashi
    • Journal Title

      PLoS ONE

      Volume: 7 Pages: 34825

    • DOI

      DOI:10.1371/journal.pone.0034825

    • Peer Reviewed
  • [Journal Article] Trade-off between oxygen and iron acquisition in bacterial cells at the air-liquid interface, FEMS Microbiol2011

    • Author(s)
      Kyosuke Yamamoto, Hiroyuki Arai, Masaharu Ishii, and Yasuo Igarashi
    • Journal Title

      Ecol

      Volume: 77 Pages: 83-94

    • DOI

      DOI:10.1111/j.1574-6941.2011.01087.x

    • Peer Reviewed
  • [Journal Article] Regulation and function of versatile aerobic and anaerobic respiratory metabolism in Pseudomonas aeruginosa2011

    • Author(s)
      Hiroyuki Arai
    • Journal Title

      Front Microbio

      Volume: 2 Pages: 103

    • DOI

      DOI:10.3389/fmicb.2011.00103

    • Peer Reviewed
  • [Journal Article] Complete genome sequence of the thermophilic, obligately chemolithoautotrophic hydrogen oxidizing bacterium Hydrogenobacter thermophilus TK-62010

    • Author(s)
      Hiroyuki Arai, Haruna Kanbe, Masaharu Ishii, and Yasuo Igarashi
    • Journal Title

      J Bacteriol

      Volume: 192(10) Pages: 2651-2652

    • DOI

      DOI:10.1128/JB.00158-10

  • [Journal Article] Differential expression of multiple terminal oxidases for aerobic respiration in Pseudomonas aeruginosa2010

    • Author(s)
      Takuro Kawakami, Miho Kuroki, Masaharu Ishii, Yasuo Igarashi and Hiroyuki Arai
    • Journal Title

      Environ Microbiol

      Volume: 12(6) Pages: 1399-1412

    • DOI

      DOI:10.1111/j.1462-2920.2009.02109.x

    • Peer Reviewed
  • [Journal Article] Daijiro Ohmori, Masaharu Ishii, and Yasuo Igarashi, Enzymatic and electron paramagnetic resonance studies of anabolic pyruvate synthesis by pyruvate : ferredoxin oxidoreductase from Hydrogenobacter thermophilus2010

    • Author(s)
      Takeshi Ikeda, Masahiro Yamamoto, Hiroyuki Arai
    • Journal Title

      FEBS J

      Volume: 277(2) Pages: 501-510

    • DOI

      DOI:10.1111/j.1742-4658.2009.07506.x

    • Peer Reviewed
  • [Journal Article] Masaharu Ishii, and Yasuo Igarashi, Ferredoxin-NADP+reductase from the thermophilic hydrogen-oxidizing bacterium, Hydrogenobacter thermophilus TK-62009

    • Author(s)
      Takeshi Ikeda, Miyuki Nakamura, Hiroyuki Arai
    • Journal Title

      FEMS Microbio. Lett

      Volume: 297 Pages: 124-130

    • DOI

      DOI:10.1111/j.1574-6968.2009.01667.x

    • Peer Reviewed
  • [Presentation] 緑膿菌におけるcbb3-type cytochrome c oxidaseのサブユニットシャッフリング2012

    • Author(s)
      長村達也、川上卓郎、石井正治、五十嵐泰夫、新井博之
    • Organizer
      日本農芸化学会大会
    • Place of Presentation
      京都女子大学(京都)
    • Year and Date
      2012-03-24
  • [Presentation] 緑膿菌における好気および嫌気呼吸代謝の多様性と調節機構2012

    • Author(s)
      新井博之
    • Organizer
      緑膿菌感染症研究会
    • Place of Presentation
      慶應義塾大学(東京)
    • Year and Date
      2012-02-18
  • [Presentation] 緑膿菌における複数のcbb3 cytochrome c oxidaseの機能解析2011

    • Author(s)
      長村達也,川上卓郎,石井正治,五十嵐泰夫,新井博之
    • Organizer
      日本生化学会大会
    • Place of Presentation
      京都国際会館(京都)
    • Year and Date
      2011-09-24
  • [Presentation] 緑膿菌における好気および嫌気呼吸酵素の特徴と遺伝子発現制御2011

    • Author(s)
      新井博之
    • Organizer
      日本生物物理学会年会
    • Place of Presentation
      兵庫県立大学(兵庫)
    • Year and Date
      2011-09-16
  • [Presentation] Characterization of multiple terminal oxidases for aerobic respiration in Pseudomonas aeruginosa2011

    • Author(s)
      Tatsuya Osamura, Takuro Kawakami, Masaharu Ishii, and Yasuo Igarashi, and Hiroyuki Arai
    • Organizer
      Pseudomonas
    • Place of Presentation
      Sydney, Australia
    • Year and Date
      2011-09-06
  • [Presentation] 好熱性水素細菌Hydrogenobacter thermophilusの好気呼吸および嫌気脱窒遺伝子の環境変化に応じた発現調節2011

    • Author(s)
      新井博之、神邉悠奈、石井正治、五十嵐泰夫
    • Organizer
      環境バイオテクノロジー学会大会
    • Place of Presentation
      東京大学(東京)
    • Year and Date
      2011-06-21
  • [Presentation] Characterization of multiple terminal oxidases for aerobic respiration in Pseudomonas aeruginosa2011

    • Author(s)
      Hiroyuki Arai, Tatsuya Osamura, Takuro Kawakami, Masaharu Ishii, and Yasuo Igarashi
    • Organizer
      ASM general meeting
    • Place of Presentation
      New Orleans, USA
    • Year and Date
      2011-05-24
  • [Presentation] 好気性光合成細菌Roseobacter denitrificans OCh114の光酸化ストレス防御に関わるσ因子の機能解析2011

    • Author(s)
      青山晴菜、木村真人、石井正治、五十嵐泰夫、新井博之
    • Organizer
      日本ゲノム微生物学会年会
    • Place of Presentation
      東北学院大学(仙台)
    • Year and Date
      2011-03-15
  • [Presentation] 好熱性水素細菌Hydrogenobacter thermophilusの好気呼吸および嫌気硝酸呼吸(脱窒)関連遺伝子の発現調節2010

    • Author(s)
      新井博之、神邉悠奈、石井正治、五十嵐泰夫
    • Organizer
      日本生化学会大会
    • Place of Presentation
      神戸ポートアイランド(神戸)
    • Year and Date
      2010-12-10
  • [Presentation] 緑膿菌の好気呼吸における末端酸化酵素のプロトン排出効率に関する研究2010

    • Author(s)
      長村達也,川上卓郎,石井正治,五十嵐泰夫,新井博之
    • Organizer
      日本生化学会大会
    • Place of Presentation
      神戸ポートアイランド(神戸)
    • Year and Date
      2010-12-10
  • [Presentation] Functional analysis of two DNR-type denitrification regulators of the aerobic photosynthetic bacterium Roseobacter denitrificans OCh1142010

    • Author(s)
      Makoto Kimura, Hiroyuki Arai, Msaharu Ishii, and Yasuo Igarashi
    • Organizer
      ASM Meeting
    • Place of Presentation
      San Diego, USA
    • Year and Date
      2010-05-25
  • [Presentation] ゲノム解析から見えてきた好熱性絶対独立栄養性水素細菌Hydrogenobacter thermophilusの特徴2009

    • Author(s)
      新井博之、石井正治、五十嵐泰夫
    • Organizer
      日本生物工学会大会
    • Place of Presentation
      名古屋大学(名古屋)
    • Year and Date
      2009-09-24
  • [Presentation] Masaharu Ishii, and Yasuo Igarashi, Transcriptome response to nitric oxide in Pseudomonas aeruginosa2009

    • Author(s)
      Miho Kuroki, Hiroyuki Arai
    • Organizer
      Pseudomonas meeting
    • Place of Presentation
      Hannover, Germany
    • Year and Date
      2009-08-14

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Published: 2013-07-31  

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