2010 Fiscal Year Final Research Report
Functional analysis of bacterial actin-like cytoskeletal proteins by using inhibitors
| Project/Area Number |
20380047
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
WACHI Masaaki Tokyo Institute of Technology, 大学院・生命理工学研究科, 准教授 (90192822)
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| Co-Investigator(Kenkyū-buntansha) |
TAKADA Ayako 東京工業大学, 技術部, 技術職員 (20401565)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 微生物学 / アクチン様細胞骨格タンパク質 |
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| Research Abstract |
It was shown that MreB helical structures were clearly visualized by staining with a newly synthesized anthracene-labeled A22 derivative in living E.coli cells. This provides the simple method observing bacterial actin-like cytoskeletons. We found that A22 induced cell lysis in E.coli mrcB mutant cells lacking PBP1B, one of the major penicillin-binding proteins. mreB(ts) mrcB double mutant lysed immediately upon temperature up-shift. These results indicate that simultaneous defects of MreB and PBP1B cause cell lysis.
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