2010 Fiscal Year Final Research Report
Development of microbial production system using bioreduction system
| Project/Area Number |
20380051
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Kyoto University |
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Principal Investigator |
KATAOKA Michihiko Kyoto University, 生命環境科学研究科, 教授 (90252494)
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| Co-Investigator(Renkei-kenkyūsha) |
OGAWA Jun 京都大学, 農学研究科, 教授 (70281102)
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| Project Period (FY) |
2008 – 2010
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| Keywords | バイオ還元 / 不斉還元 / 補酵素再生 / 旧黄色酵素 / モノオキシゲナーゼ |
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| Research Abstract |
Bioreduction system using an E. coli transformant co-expressing NAD(P)H-dependent enzyme and cofactor regeneration enzyme genes was applied to the production of other useful compounds. The reactions requiring NAD(P)H as a cofactor, such as C=C bond reductions and monooxigenation were exammined.
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[Journal Article] Expression, purification, crystallization and preliminary X-ray analysis of conjugated polyketone reductase C2 (CPR-C2) from Candida parapsilosis IFO 0708.2009
Author(s)
A. Yamamura, S. Maruoka, J. Ohtsuka, T.Miyakawa, K. Nagata, M.Kataoka, N. Kitamura, S.Shimizu, M.Tanokura
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Journal Title
Acta Crystal. F65
Pages: 1145-1148
Peer Reviewed
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