Analysis of structural elements controlling transglycosylation and its application

2010 Fiscal Year Final Research Report

• Project/Area Number: 20380056
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied biochemistry
• Research Institution: Hokkaido University
• Principal Investigator: KIMURA Atsuo Hokkaido University, 大学院・農学研究院, 教授 (90186312)
• Co-Investigator(Kenkyū-buntansha): OKUYAMA Masayuki 北海道大学, 大学院・農学研究院, 助教 (00344490)
• Project Period (FY): 2008 – 2010
• Keywords: 糖転移 / 構造因子 / 分子解析

Research Abstract

Glycosylases are enzymes, which are currently utilized for the production of oligosaccharides. Interestingly, production-ability is dependent on the kinds of glycosylases, meaning that their structural elements contribute to the reaction specificity. Our research aims at elucidating structural elements of glycosylase-catalyzed transglycosylation. Our research achieved i) elucidation of structural elements contributing to the oligosaccharide production ; ii) establishment of the theoretical background of oligosaccharide production ; iii) finding the ways to regulate the oligosaccharide production.

Research Products

• [Journal Article] Bioengineered glucansucrase of Leuconostoc mesenteroides for preferred oligosaccharide synthesis using sucrose (2011)
  • Author(s): Kang HK, Kimura A, Kim D
  • Journal Title: J Agric Food Chem (in press) Pages: IF=2.469
• [Journal Article] Truncation of N- and C-terminal regions of Streptococcus mutans dextranase enhances catalytic activity (2011)
  • Author(s): Kim YM, Shimizu R, Nakai H, Mori H, Okuyama M, Kang MS, Fujimoto Z, Funane K, Kim D, Kimura A
  • Journal Title: Appl Microbiol Biotechnol (in press) Pages: IF=2.896
• [Journal Article] Function of C-terminal region of cycloisomaltooligosaccharide glucanotransferase from Bacillus circulams T-3040 (2011)
  • Author(s): Funane K, Kawabata Y, Kim YM, Kang HK, Fujimoto Z, Kimura A, Kobayashi M
  • Journal Title: Biochim Biophys Acta 1814 Pages: 428-434, IF=2.480
• [Journal Article] Suicide substrate-based inactivation of endodextranase by 1-epoxyalkyl <-D-glucopyranosides. (2010)
  • Author(s): Kang HK, Kim YM, Nakai H, Kang MS, Hakamada W, Okuyama M, Mori H, Nishio T, Kimura A
  • Journal Title: J Appl Glycosci 57 Pages: 111-117
• [Journal Article] Optimal expression and characterization of a fusion enzyme having dextransucrase and dextranase activities (2010)
  • Author(s): Ryu HJ, Jin X, Lee JH, Woo HJ, Kim YM, Kim GJ, Seo ES, Kang HK, Kim J, Cho DL, Kimura A, Kim D
  • Journal Title: Enzyme Microb Technol 47 Pages: 212-215, IF=2.638
• [Journal Article] Ketudat-Cairns JR : Rice Os4BGlu12 is a wound-induced [○!R]-glucosidase that hydrolyzes cell-wall-[○!R]-glucan-derived oligosaccharides and glycosides. (2010)
  • Author(s): Opassiri R, Maneesan J, Akiyama T, Pomthong B, Jin S, Kimura A
  • Journal Title: Plant Sci 179 Pages: 273-280, IF=2.050
• [Journal Article] Catalytic reaction mechanism based on α-secondary deuterium isotope effects in hydrolysis of trehalose by European honeybee trehalase. (2009)
  • Author(s): Mori H, Lee JH, Okuyama M, Nishimoto M, Oguchi M, Kim D, Kimura A, Chiba S
  • Journal Title: Biosci Biotechnol Biochem 73 Pages: 2466-2473, IF=1.326
• [Journal Article] The first α-1,3-glucosidase from bacterial origin belonging to glycoside hydrolase family 31. (2009)
  • Author(s): Kang MS, Okuyama M, Mori H, Kimura A
  • Journal Title: Biochimie 91 Pages: 1434-1442, IF=3.897
• [Journal Article] Catalytic mechanism of retaining α-galactosidase belonging to glycoside hydrolase family 97. (2009)
  • Author(s): Okuyama M, Kitamura M, Hondoh H, Kang MS, Mori H, Kimura A, Tanaka I, Yao M
  • Journal Title: J Mol Biol 392 Pages: 1232-1241, IF=3.871
• [Journal Article] Structural comparison of Streptococcus mutans dextran glucosidase with glucoside hydrolases in GH13. (2009)
  • Author(s): Hondoh H, Otsuka-Rachi H, Saburi W, Mori H, Okuyama M, Kimura A
  • Journal Title: J Appl Glycosci 56(2) Pages: 111-117
• [Journal Article] Construction of a fusion enzyme of dextransucrase and dextranase : Application for one-step synthesis of isomaltooligosaccharides. (2009)
  • Author(s): Kim YM, Seo MY, Kang HK, Kimura A, Kim D
  • Journal Title: Enzyme Microb Technol 44 Pages: 159-164, IF=2.638
• [Journal Article] Structural and functional analysis of a glycoside hydrolase family 97 enzyme from Bacteroides thetaiotaomicron (2008)
  • Author(s): Kitamura M, Okuyama M, Tanzawa F, Mori H, Kitago Y, Watanabe N, Kimura A, Tanaka I, Yao M
  • Journal Title: J Biol Chem 283 Pages: 36328-36337, IF=5.520
• [Journal Article] Isolation of Bacillus and Paenibacillus bacterial strains that produce large molecular of cyclic isomaltooligosaccharides. (2008)
  • Author(s): Funane K, Terasawa K, Mizuno Y, Ono H, Gibu S, Tokashiki T, Kawabata Y, Kim YM, Kimura A, Kobayashi M
  • Journal Title: Biosci Biotechnol Biochem 72 Pages: 3277-3280, IF=1.390
• [Journal Article] Enzymatic synthesis of α-2-deoxyglucosyl derivatives catalyzed by organic solvent-resistant α-glucosidase. (2008)
  • Author(s): Kim YM, Kim D, Kimura A
  • Journal Title: Biotechnol Bioprocess Eng 13 Pages: 639-645, IF=1.653
• [Journal Article] Structure-function relationship of substrate length specificity of dextran glucosidase from Streptococcus mutans. (2008)
  • Author(s): Saburi W, Hondoh H, Kim YM, Mori H, Okuyama M, Kimura A
  • Journal Title: Biologia 63 Pages: 1000-1005, IF=0.406
• [Journal Article] Substrate recognition of Escherichia coli YicI (α-xylosidase) (2008)
  • Author(s): Okuyama M, Kang MS, Yaoi K, Mitsuishi Y, Mori H, Kimura A
  • Journal Title: J Appl Glycosci 55 Pages: 111-118
• [Journal Article] Substrate recognition of α-1,6-glucosidic linkage hydrolyzing enzyme, dextran glucosidase from Streptococcus mutans (2008)
  • Author(s): Hondoh H, Saburi W, Mori H, Okuyama M, Nakada T, Matsuura Y, Kimura A
  • Journal Title: J Mol Biol 378 Pages: 911-920, IF=4.146
• [Presentation] Structural element determining the diverse specificities for chain-length of substrate in glycoside hydrolase family 31 α-glucosidases. (2011)
  • Author(s): Tagami T, Okuyama M, Mori H, Kimura A
  • Organizer: 9th Carbohydrate Bioengineering Meeting.
  • Place of Presentation: Calouste Gulbenkian Foundation (Lisbon, Portugal)(ポスター賞1位を受賞)
  • Year and Date: 20110515-20110518
• [Presentation] Molecular Mechanism of novel enzymes degrading and forming α-1,6-glucosidic linkages, including megalosaccharide production. (2011)
  • Author(s): Kim YM, Kanegae M, Hondoh H, Nishimura T, Saburi W, Sadahiro J, Lang W, Shimizu R, Nakai H, Kang HK, Okuyama M, Mori H, Kang MS, Fujimoto Z, Funane K, Kim D, Kimura A
  • Organizer: The 78th Annual Meeting and International Symposium in 2011 Annual Conference of Korean Society of Food Science and Technology.
  • Place of Presentation: Daegu Exhibition Convention Center (Daegu, Korea).
  • Year and Date: 2011-06-09
• [Presentation] Putative role of Tyr227, Gln349 and Leu350 near catalytic amino acids of honeybee α-glucosidase III (2010)
  • Author(s): Ngiwsara L, Iwai G, Mori H, Okuyama M, Kimura A
  • Organizer: 4th Symposium on the Alpha-Amylase Family
  • Place of Presentation: Smolenice Cattle (Smolenice, Slovakia)(ポスター賞3位を受賞)
  • Year and Date: 20100925-20100930
• [Presentation] Shifting specificity of Aspergillus niger α-glucosidase from short chain substrates to long chain substrates (2010)
  • Author(s): Tagami T, Nishimura T, Okuyama M, Mori H, Kimura A
  • Organizer: Plant Polysaccharide and Applied Glycoscience Workshop 2010
  • Place of Presentation: Sasagawa Hall (Tokyo, Japan).
  • Year and Date: 20100729-20100731
• [Presentation] 糖質酵素の基礎研究とその展開 (2010)
  • Author(s): 木村淳夫
  • Organizer: 日本応用糖質科学会平成22年度大会(第59回)
  • Place of Presentation: グランシップ(静岡県)
  • Year and Date: 2010-09-17
• [Presentation] α-グリコシダーゼの機能と構造に関する研究 (2010)
  • Author(s): 奥山正幸
  • Organizer: 日本農芸化学会2010年度大会
  • Place of Presentation: 東京大学(東京都)(日本農芸化学会奨励賞受賞講演)
  • Year and Date: 2010-03-28
• [Presentation] Transglucosylation pattern of wild-type and specificity-altered dextran glucosidase (2009)
  • Author(s): Hondoh H, Otsuka-Rachi H, Saburi W, Mori H, Okuyama M, Kimura A
  • Organizer: 15th European Carbohydrate Symposium
  • Place of Presentation: University of Vienna (Vienna, Austria)
  • Year and Date: 20090719-20090724
• [Presentation] Novel cycloisomaltooligosaccharides of large molecule found in culture supernatant of Bacillus and Paenibacillus strains (2008)
  • Author(s): Funane K, Ono H, Gibu S, Tokashiki T, Kawabata Y, Kim Y-M, Kimura A, Kobayashi M
  • Organizer: 24th International Carbohydrate Symposium
  • Place of Presentation: University of Oslo (Oslo, Norway)
  • Year and Date: 20080727-20080801
• [Presentation] Identification of essential catalytic residues in an Escherichia coli trehalase and an inverting glycosynthase reaction catalyzed by the catalytic basemutated enzyme (2008)
  • Author(s): Mori H, Saito S, Ogawa K, Maki M, Okuyama M, Kimura A
  • Organizer: 24th International Carbohydrate Symposium(July 27th to August 1st)
  • Place of Presentation: University of Oslo (Oslo, Norway)
  • Year and Date: 20080727-20080801
• [Book] Carbohydrate-active enzymes : structure, function, and applications(ed.by Park K-H)(Molecular mechanism of α-glucosidase.) (2008)
  • Author(s): Okuyama M, Mori H, Hondoh H, Nakai H, Saburi W, Kang MS, Kim YM, Nishimoto M, Wongchawalit J, Yamamoto T, Son M, Lee JH, Mar SS, Fukuda K, Chiba S, Kimura A
  • Total Pages: 64-76
  • Publisher: Woodhead Publishing Ltd. (Cambridge, UK)
• [Remarks] (1)アウトリーチ活動情報 (1)奥山正幸、木村淳夫:独立行政法人科学技術振興機構・平成22年度サイエンス・パートナーシップ・プロジェクト(SPP)事業(北海道札幌藻岩高校) (2)奥山正幸、木村淳夫:独立行政法人科学技術振興機構・平成21年度サイエンス・パートナーシップ・プロジェクト(SPP)事業(北海道札幌藻岩高校) (3)奥山正幸、木村淳夫:独立行政法人科学技術振興機構・平成20年度サイエンス・パートナーシップ・プロジェクト(SPP)事業(北海道札幌藻岩高校) (2)ホームページ等
  • URL: http://hecate.general.hokudai.ac.jp/welcome/toppage-jpn.html