Molecular system of membrane transport and level regulation of metal ions

2010 Fiscal Year Final Research Report

• Project/Area Number: 20380060
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied biochemistry
• Research Institution: Nagoya University
• Principal Investigator: MAESHIMA Masayoshi Nagoya University, 生命農学研究科, 教授 (80181577)
• Project Period (FY): 2008 – 2010
• Keywords: 亜鉛 / 亜鉛輸送体 / カルシウム / カチオン結合タンパク質 / 液胞膜 / 膜輸送体 / プロトンポンプ

Research Abstract

I investigated Arabidopsis vacuolar membrane zinc transporter AtMTP1, novel cation-biding proteins PCaP1 and PCaP2, and H^+-pyrophosphatase (H^+-PPase), which are involved in metal homeostasis in plant cells.
(1) AtMTP1 works as a Zn^<2+>/H^+ exchanger and has high affinity for zinc and zinc selectivity. We determined functional residues related to ion transport and selection of zinc.
(2) PCaP1 and PCaP2 N-myristoylated are stably associated with plasma membrane. I found that PCaP1 is involved in copper sensitivity and stomatal closer and PCaP2 in development of root hairs.
(3) I clarified that Arabidopsis type I H^+-PPase is localized to the vacuolar membrane and mainly contributes to metal homeostasis through activation of metal active transporters. The type II H^+-PPase is a minor isoform and localized to the Golgi membranes.

Research Products

• [Journal Article] The iTRAQ analysis reveals mechanisms of growth defects due to excess zinc in Arabidopsis thaliana. (2011)
  • Author(s): Fukao, Y., Ferjani, A., Tomioka, R., Nagasaki, N., Kurata, R., Nishimori, Y., Fujiwara, M., Maeshima, M.
  • Journal Title: Plant Physiology In press Pages: DOI : 10.1104/pp.110.169730
  • Peer Reviewed
• [Journal Article] An Arabidopsis hydrophilic Ca2^+-binding protein with a PEVK-rich domain, PCaP2, is associated with the plasma membrane and interacts with calmodulin and phosphatidylinositol phosphates. (2010)
  • Author(s): Kato, M., Nagasaki-Takeuchi, N., Ide, Y., Tomioka, R., Maeshima, M.
  • Journal Title: Plant Cell Physiol. 51 Pages: 366-379
  • Peer Reviewed
• [Journal Article] PCaPs, possible regulators of PtdInsP signals on plasma membrane (2010)
  • Author(s): Kato, M., Nagasaki-Takeuchi, N., Ide, Y.,Tomioka, R., Maeshima, M.
  • Journal Title: Plant Signaling & Behavior. 5 Pages: 848-850
  • Peer Reviewed
• [Journal Article] Quantification, organ-specific accumulation and intracellular localization of type II H^+-pyrophosphatase in Arabidopsis thaliana. (2010)
  • Author(s): Segami, S., Nakanishi, Y., Sato, H.M., Maeshima, M.
  • Journal Title: Plant Cell Physiol. 51 Pages: 1350-1360
  • Peer Reviewed
• [Journal Article] NIP1;1, an aquaporin homolog, determines the arsenite sensitivity of Arabidopsis thaliana. (2009)
  • Author(s): Kamiya, T., Tanaka, M., Mitani, N., Ma, J.F., Maeshima, M., Fujiwara, T.
  • Journal Title: J.Biol.Chem. 284 Pages: 2114-2120
  • Peer Reviewed
• [Journal Article] AtHMA1 contributes to detoxification of excess Zn(II) in Arabidopsis. (2009)
  • Author(s): Kim, Y.-Y., Choi, H., Segami, S., Cho, H.-T., Martinoia, E., Maeshima, M., Lee, Y.
  • Journal Title: Plant J. 58 Pages: 737-753
  • Peer Reviewed
• [Journal Article] A mutant strain Arabidopsis that lacks vacuolar membrane zinc transporter MTP1 revealed the latent tolerance to excessive zinc. (2009)
  • Author(s): Kawachi, M., Mori, H., Kobae, Y., Tomioka, R., Maeshima, M.
  • Journal Title: Plant Cell Physiol. 50 Pages: 1156-1170
  • Peer Reviewed
• [Journal Article] Synchrony between flower opening and petal-color change from red to blue in morning glory, Ipomoea tricolor cv.Heavenly Blue. (2009)
  • Author(s): Yoshida, K., Miki, N., Momonoi, K., Kawachi, M., Katou, K., Okazaki, Y., Uozumi, N., Maeshima, M., Kondo
  • Journal Title: Proceedings of the Japan Academy (Ser.B, Physical and Biological Sciences) B85(6) Pages: 187-197
  • Peer Reviewed
• [Journal Article] Functional enhancement by single-residue substitution of Streptomyces coelicolor A3(2) H^+-translocating pyrophosphatase. (2009)
  • Author(s): Hirono, M., Maeshima, M.
  • Journal Title: J.Biochem. 145 Pages: 617-621
  • Peer Reviewed
• [Journal Article] Dynamic aspects of ion accumulation by vesicle traffic under salt stress in Arabidopsis. (2009)
  • Author(s): Hamaji, K., Nagira, M., Yoshida, K., Ohnishi, M., Oda, Y., Uemura, T., Goh, T., Sato, M.-H., Terao-Morita, M., Tasaka, M., Hasezawa, S., Nakano, A., Hara-Nishimura, I., Maeshima, M., Fukaki, H., Mimura, T.
  • Journal Title: Plant Cell Physiol. 50 Pages: 2023-2033
  • Peer Reviewed
• [Journal Article] Deletion of a histidine-rich loop of AtMTP1, a vacuolar Zn^2+/H^+ antiporter of Arabidopsis thaliana, stimulates the transport activity. (2008)
  • Author(s): Kawachi, M., Kobae, Y., Mimura, T., Maeshima, M.
  • Journal Title: J.Biol.Chem. 283 Pages: 8374-8383
  • Peer Reviewed
• [Journal Article] Characterization of a tobacco TPK-type K^+ channel as a novel tonoplast K+ channel using yeast tonoplast. (2008)
  • Author(s): Hamamoto, S., Marui, J., Matsuoka, K., Higashi, K., Igarashi, K., Nakagawa, T., Mori, Y., Murata, Y., Nakanishi, Y., Maeshima, M., Yabe, I., Uozumi, N.
  • Journal Title: J.Biol.Chem. 283 Pages: 1911-1920
  • Peer Reviewed
• [Journal Article] N-myristoylation and association with phosphatidylinositol phosphates (2008)
  • Author(s): Nagasaki, N., Tomioka, R., Maeshima, M.
  • Journal Title: PtdIns(3,4,5)P3 and PtdIns(3,5)P2. FEBS J. 275 Pages: 2267-2282
  • Peer Reviewed
• [Journal Article] The ABC transporter AtABCB14 is a malate importer and modulates stomatal response to CO2 (2008)
  • Author(s): Lee, M., Choi, Y., Burla, B., Kim, Y.-Y., Jeon, B., Maeshima, M., Yoo, J.-Y., Martinoia, M., Lee, Y.
  • Journal Title: Nature Cell Biology 10 Pages: 1217-1223
  • Peer Reviewed
• [Journal Article] A plasma membrane-associated protein of Arabidopsis thaliana AtPCaP1 binds copper ions and changes its higher order structure. (2008)
  • Author(s): Nagasaki-Takeuchi, N., Miyano, M., Maeshima, M.
  • Journal Title: J.Biochem. 144 Pages: 487-497
  • Peer Reviewed
• [Presentation] 植物細胞膜に局在する天然変性タンパク質PCaP、シンポジウム「"ひらひら"した不定形の領域を介する蛋白相互作用:医薬、農薬の新たな探索標的」 (2011)
  • Author(s): 長崎菜穂子、加藤真理子、永田千咲子、宮野雅司、前島正義
  • Organizer: 日本農芸化学会2011年度大会(要旨集記載.東北関東大震災のため大会中止となったが、大会は成立した.)
  • Place of Presentation: 京都
  • Year and Date: 20110325-20110328
• [Presentation] Crosstalk between zinc and iron homeostasis : iTRAQ analysis and zinc transporters. (2011)
  • Author(s): fukao Y., kawachi, M., kogawa, S., tomioka, R., mimura, T., Kramer, U., Maeshima M.
  • Organizer: The Winter Conference
  • Place of Presentation: POSTECH, Pohang, Korea
  • Year and Date: 20110110-20110111
• [Presentation] Structural, functional property of the His-rich region of Arabidopsis vacuolar membrane Zn transporter AtMTP1.International Symposium on Protein structure and dynamics : from molecules to assembly. (2010)
  • Author(s): Kawachi, M., Kogawa, S., Kraemer, K., Mimura, T., Maeshima M.
  • Organizer: GCOE/Structural Biology Research Center
  • Place of Presentation: Nagoya University
  • Year and Date: 20101123-20101124
• [Presentation] Molecular physiological analysis of His-rich region of Arabidopsis vacuolar membrane Zn transporter AtMTP1 (2010)
  • Author(s): Kawachi, M., Kogawa, S., Kraemer, K., Mimura, T., Maeshima M.
  • Organizer: The 60th Fujiwara Symposium on "Zinc Signal and Cellular Function".
  • Place of Presentation: Osaka, Japan
  • Year and Date: 20101029-20101031
• [Presentation] A novel, hydrophilic cation-binding protein PCaP1 is stably associated with plasma membrane and is involved in regulation of stomatal apertures. (2010)
  • Author(s): 永田千咲子, 加藤真理子, Kinoshita, T., Maeshima M.
  • Organizer: 21st International Conference on Arabidopsis Research.
  • Place of Presentation: Yokohama
  • Year and Date: 20100706-20100710
• [Presentation] H^+-pyrophosphatase is localized in the membrane of vacuole and the bulb-like structure. (2010)
  • Author(s): Segami, S., Makino, S., Maeshima M.
  • Organizer: 21st International Conference on Arabidopsis Research.
  • Place of Presentation: Yokohama
  • Year and Date: 20100706-20100710
• [Presentation] Structure-function relationship and physiological meanings of H^+-pyrophosphatase. (2009)
  • Author(s): Hirono, M., Segami, S., Muto, Y., Kawachi, M., Maeshima M.
  • Organizer: The XII Meeting of the SBFV (The Brazilian Society of Plant Physiology)(presentation, Sep.9)
  • Place of Presentation: Fortaleza, Brazil
  • Year and Date: 20090907-20090912
• [Presentation] A hydrophilic Ca2+-binding protein with PEVK-rich domain, PCaP2, is associated with plasma membrane via N-myristoylation and interacts with calmodulin and phosphatidylinositol phosphates in Arabidopsis thaliana. (2009)
  • Author(s): Kato M., Nagasaki-Takeuchi N, Ide, U., Maeshima M.
  • Organizer: Plant Biology 2009
  • Place of Presentation: Hawaii, USA
  • Year and Date: 20090719-20090722
• [Presentation] Analysis of Ca2+-binding proteins, CCaP1 and CCaP2, in Arabidopsis thaliana. (2009)
  • Author(s): Ouchi, Y., Nagatani, A., Maeshima M.
  • Organizer: Plant Biology 2009
  • Place of Presentation: Hawaii, USA
  • Year and Date: 20090719-20090722
• [Presentation] Structure-function relationship of AtMTP1, a vacuolar Zn^2+/H+ antiporter and defense mode against zinc stress in atmtp1 mutant plant. (2008)
  • Author(s): Kawachi, M., Mori, H., Maeshima M.
  • Organizer: The GRL Symposium on Phytoremediation. Pohang University of Science and Technology
  • Place of Presentation: Pohang
  • Year and Date: 20080712-20080714
• [Presentation] Molecular characterization of a novel Ca^2+-binding protein of Arabidopsis thaliana PCaP2 associated with plasma membrane via N-myristoylation. (2008)
  • Author(s): Kato M., Nagasaki N., Ide, Y., Maeshima M.
  • Organizer: Plant Biology 2008.
  • Place of Presentation: Merida, Mexico
  • Year and Date: 20080626-20080701
• [Presentation] Deletion of a histidine-rich loop of AtMTP1, a vacuolar Zn^2+/H^+ antiporter of Arabidopsis thaliana, stimulates the transport activity. (2008)
  • Author(s): Kawachi, M., Kobae, Y., Mimura, T., Maeshima M.
  • Organizer: Plant Biology 2008.
  • Place of Presentation: Merida, Mexico
  • Year and Date: 20080626-20080701
• [Book] In A Soil Biology Series : Detoxification of Heavy Metals(The role of membrane transport in the detoxification and accumulation of zinc in plants) (2011)
  • Author(s): 河内美樹, Kobae, Y., Tomioka, R., Maeshima M.
  • Publisher: Springer-Verlag(In Press)
• [Book] 生物の事典(石原勝敏、末光隆志編)(4.2.7.植物に含まれる物質) (2010)
  • Author(s): 河内美樹、前島正義
  • Total Pages: 109-114
  • Publisher: 朝倉書店