2010 Fiscal Year Final Research Report
Protein engineering and reaction control technology targeting thermolysin for the expansion of its use in food industry
| Project/Area Number |
20380061
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied biochemistry
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| Research Institution | Kyoto University |
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Principal Investigator |
INOUYE Kuniyo Kyoto University, 大学院・農学研究科, 教授 (10223249)
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| Co-Investigator(Kenkyū-buntansha) |
YASUKAWA Kiyoshi 京都大学, 大学院・農学研究科, 准教授 (30397559)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 酵素化学酵素利用学 |
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| Research Abstract |
Thermolysin (TLN) is a thermostable neutral metalloproteinase produced in the culture broth of Bacillus thermoproteolyticus. It is widely used for the peptide bond formation through reverse reaction of hydrolysis. In this study, for the expansion of the use of TLN in food industry, we increased the activity and stability of TLN and altered its substrate specificity using protein engineering and reaction control technology.
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