2010 Fiscal Year Final Research Report
Development of a synthetic method for hydrophobic glycoprotein and its application to saposin synthesis
| Project/Area Number |
20380069
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bioproduction chemistry/Bioorganic chemistry
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| Research Institution | Tokai University |
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Principal Investigator |
HOJO Hironobu Tokai University, 工学部, 教授 (00209214)
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| Co-Investigator(Renkei-kenkyūsha) |
NAKAHARA Yoshiaki 東海大学, 工学部, 教授 (50087574)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 疎水性糖タンパク質 / セグメント縮合 / O-アシルイソペプチド |
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| Research Abstract |
The synthesis of highly hydrophobic glycoproteins, such as membrane proteins and lipid-binding proteins, is extremely difficult even with the use of efficient segment coupling methods. The main difficulty is that the segments usually retain low solubility during peptide synthesis, purification and ligation steps. To overcome this problem, a method to enhance the solubility of segments during peptide synthesis and purification was developed and applied to the synthesis of lipid-binding protein, saposin C.
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Research Products
(52 results)
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[Journal Article] Direct binding of gangliosides to Helicobacter pylori vacuolating cytotoxin (VacA) neutralizes its toxin activity2010
Author(s)
A.Wada, M.Hasegawa, P.-F.Wong, E.Shirai, L.-J.Tan, R.Llanes, H.Hojo, E.Yamasaki, A.Ichinose, Y.Ichinose, M.Senba
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Journal Title
Glycobiology 20
Pages: 668-678
Peer Reviewed
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