2010 Fiscal Year Final Research Report
Development of renin inhibitor screening system and structure-function relationship of renin inhibitor(s) from food stuffs
| Project/Area Number |
20380081
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Food science
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| Research Institution | Akita Prefectural Agriculture, Forestry and Fisheries Research Center (2008, 2010)
秋田県総合食品研究所 (2009) |
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Principal Investigator |
TAKAHASHI Saori Akita Prefectural Agriculture, Forestry and Fisheries Research Center, 食品加工研究所, 所長 (10142184)
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| Co-Investigator(Kenkyū-buntansha) |
HORI Kazuyuki 秋田県総合食品研究センター, 企画マーケティング班, 上席研究員 (50181516)
GOTOH Takeshi 秋田大学, 工学資源学部, 教授 (10215494)
YOSHIZAWA Yuko 秋田県立大学, 生物資源科学部, 教授 (20269202)
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| Project Period (FY) |
2008 – 2010
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| Keywords | レニン / アンギオテンシン / 血圧調節 / 大豆 / ソヤサポニン / 米 / オレイン酸 / リノール酸 |
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| Research Abstract |
We succeeded effective production of recombinant human renin by baculovirus-insect cell expression system. Using recombinant human renin as a target enzyme, we found renin inhibitory activity in soybean and isolated soybean renin inhibitor. The physico-chemical data on the isolated inhibitor were identical with those of soyasaponin I. In addition, we studied the effects of various saponins on recombinant human renin activity and found that so-called glucuronide saponin which glucuronic acid connected to the third position of sapogenol was renin inhibitory saponins. Furthermore, we found the existence of the renin inhibitor to rice and identified the structure with oleic acid and linoleic acid.
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[Journal Article] Reduction of blood pressure by soybean saponins, renin inhibitor from soybean, in spontaneously hypertensive rats.2010
Author(s)
Kazuyuki Hiwatashi, Hitoshi Shirakawa, Kazuyuki Hori, Yumiko Yoshiki, Nao Suzuki, Michio Kimao, Saori Takahashi
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Journal Title
Bioscience, Biotechnology, and Biochemistry 74
Pages: 2310-2312
Peer Reviewed
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[Journal Article] The occurrence of renin inhibitor in rice : Isolation, identification, and, structure and function.2010
Author(s)
Saori Takahashi, Tetsuo Tokiwano, Keishi Hata, Mika Hokari, Nao Suzuki, Ikuko Kodama, Yuko Yoshizawa, Takeshi Gotoh
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Journal Title
Bioscience, Biotechnology, and Biochemistry 74
Pages: 1713-1715
Peer Reviewed
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