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2010 Fiscal Year Final Research Report

Comprehensive analysis to determine chemical structures in supercoolingpromoting substances from xylem parenchyma cells of trees and considerations to obtain much volume of supercooling-promoting substances for their applications

Research Project

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Project/Area Number 20380099
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Wood science
Research InstitutionHokkaido University

Principal Investigator

FUJIKAWA Seizo  Hokkaido University, 名誉教授 (50091492)

Co-Investigator(Kenkyū-buntansha) FUKUSHI Yukiharu  北海道大学, 大学院・農学研究院, 講師 (60218906)
ARAKAWA Keita  北海道大学, 大学院・農学研究院, 准教授 (00241381)
Project Period (FY) 2008 – 2010
Keywords抽出成分 / 微量成分 / 木部柔細胞
Research Abstract

From xylem parenchyma cells in trees that can supercool to -40C during winter, we identified a several kinds of flavonol glycosides and hydrolysable tannins. Based on such results, we could also identified more than 50 kinds of flavonol and tannin-related compounds that promoted supercooling of water. We also tried to use these supercooling-promoting substances as novel materials that inhibit freezing of water.

  • Research Products

    (18 results)

All 2011 2010 2009 2008 Other

All Journal Article (9 results) (of which Peer Reviewed: 8 results) Presentation (6 results) Remarks (1 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] Successful introduction of novel supercoolant, kaempferol-7-0-beta-D- glucopyranoside (KF7G), to sub-zero non-freezing rat heart preservation2010

    • Author(s)
      Shimada S, Motomura N, Kinoshita O, saito A, Kasuga J, Matsusaka Y, Kawabata J, Kuwabara C, Fujikawa S, Ono M
    • Journal Title

      Low Temperature Medicine 36

      Pages: 20-24

    • Peer Reviewed
  • [Journal Article] Analysis of supercooling-facilitating (anti-ice nucleation) activity of flavonol glycosides2010

    • Author(s)
      Kasuga J, Fukushi Y, Kuwabara C, Wang D, Nishioka A, Fujikawa E, Arakawa K, Fujikawa S
    • Journal Title

      Cryobiology 60

      Pages: 240-243

    • Peer Reviewed
  • [Journal Article] Endoplasmic localized-small heat shock protein that accumulates in mulberry tree (Morus bombycis koids.) during seasonal cold acclimation is responsive to abscisic acid2010

    • Author(s)
      Ukaji N, Kuwabara C, Kanno Y, Seo M, Takezawa D, Arakawa K, Fujikawa S
    • Journal Title

      Tree Physiology 30

      Pages: 502-513

    • Peer Reviewed
  • [Journal Article] Cryo-scanning electron microscopic study on freezing behaviors of tissue cells in dormant buds of larch (Larix kaempferi)2009

    • Author(s)
      Endoh K, Kasuga J, Arakawa K, Fujikawa S
    • Journal Title

      Cryobiology 59

      Pages: 214-222

    • Peer Reviewed
  • [Journal Article] Factors related to change of deep supercooling capability in xylem parenchyma cells of trees2009

    • Author(s)
      Fujikawa S, Kasuga J, Takata N, Arakawa K
    • Journal Title

      Plant Cold Hardiness ; From the Laboratory to the Field(Gusta LV, Wisniewski ME, Tanino K (eds), CABI Press)

      Pages: 29-42

    • Peer Reviewed
  • [Journal Article] 樹木の凍結抵抗性-凍らない水-2009

    • Author(s)
      藤川清三、春日純、荒川圭太
    • Journal Title

      低温生物工学会誌 55

      Pages: 37-41

    • Peer Reviewed
  • [Journal Article] Improved cryopreservation by diluted vitrification solution with supercooling-facilitating flavonol glycoside2008

    • Author(s)
      Kami D, Kasuga J, Arakawa K, Fujikawa S
    • Journal Title

      Cryobiology 57

      Pages: 242-245

    • Peer Reviewed
  • [Journal Article] Deep supercooling xylem parenchyma cells of katsura tree (Cercidiphyllum japonicum) contain flavonol glycosides exhibiting high anti-ice nucleation activity2008

    • Author(s)
      Kasuga J, Hashidoko Y, Nishioka A, Yoshiba M, Arakawa K, Fujikawa S
    • Journal Title

      Plant, Cell and Environment 31

      Pages: 1335-1348

    • Peer Reviewed
  • [Journal Article] 過冷却促進物質2008

    • Author(s)
      藤川清三、春日純
    • Journal Title

      検査と技術 36

      Pages: 546-549

  • [Presentation] 水を凍らせづらくする物質-過冷却促進(氷核形成阻害)物質-2010

    • Author(s)
      藤川清三
    • Organizer
      伝熱学会北海道支部会
    • Place of Presentation
      北海道大学工学部・札幌
    • Year and Date
      2010-12-10
  • [Presentation] 樹木の寒冷環境適応機構とその産業的応用の可能性2010

    • Author(s)
      藤川清三
    • Organizer
      岩手大学CRCセミナー
    • Place of Presentation
      岩手大学・盛岡
    • Year and Date
      2010-11-17
  • [Presentation] Unfrozen water in trees2010

    • Author(s)
      藤川清三
    • Organizer
      Special Seminar at School of Life Science in Lanzhou University
    • Place of Presentation
      中国・蘭州大学
    • Year and Date
      2010-05-21
  • [Presentation] Application of anti-ice nucleation substances from deep supercooling xylem parenchyma cells in trees in order to regulate freezing condition for cryopreservation2009

    • Author(s)
      藤川清三
    • Organizer
      1^<st> International Symposium on Cryopreservation in Horticultural Speciess
    • Place of Presentation
      アレンベルグ城・ベルギー
    • Year and Date
      2009-04-07
  • [Presentation] 深過冷却する樹木細胞中の過冷却促進(氷核形成阻害)物質2009

    • Author(s)
      藤川清三
    • Organizer
      日本冷凍空調学会セミナー
    • Place of Presentation
      きゅりあん・品川区
    • Year and Date
      2009-01-16
  • [Presentation] 樹木の凍結抵抗性-凍らない水-2008

    • Author(s)
      藤川清三
    • Organizer
      低温生物工学会セミナー
    • Place of Presentation
      石川県立大学・石川県
    • Year and Date
      2008-06-13
  • [Remarks] アウトリーチ活動 (1)藤川清三:樹木の中の凍らない水、市民公開農学特別講座2009年11月27日、北海道大学農学部・札幌 (2)藤川清三:樹の中の凍らない水。何故?、九大・北大フロンティアセミナー、2009年10月20日、東京コンファレンスステーション・東京 ホームページ等

  • [Patent(Industrial Property Rights)] 過冷却促進剤2011

    • Inventor(s)
      藤川清三・福士幸治・荒川圭太
    • Industrial Property Rights Holder
      北海道大学・アミノアップ化学
    • Industrial Property Number
      特許, PCT/JP2011/054892
    • Filing Date
      2011-03-03
  • [Patent(Industrial Property Rights)] Supercooling-promoting agent2009

    • Inventor(s)
      藤川清三・春日純・橋床泰之・福士幸治・荒川圭太
    • Industrial Property Rights Holder
      北海道大学・オリンパス
    • Industrial Property Number
      特許, PCT/JP2007/063784
    • Filing Date
      2009-08-01

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Published: 2012-01-26   Modified: 2016-04-21  

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