Study on shrimp immune system against microbial pathogens

2010 Fiscal Year Self-evaluation Report

• Project/Area Number: 20380109
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General fisheries
• Research Institution: Tokyo University of Marine Science and Technology
• Principal Investigator: HIRONO Ikuo Tokyo University of Marine Science and Technology, 大学院・海洋科学技術研究科, 教授 (00270926)
• Project Period (FY): 2008 – 2011
• Keywords: クルマエビ / 免疫 / 生体防御 / RNA干渉

Research Abstract

(1)血球凝集因子の発現をRNA干渉(RNAi)法により抑制した際に、遺伝子発現が抑制される遺伝子の特定と、そのメカニズムの解明
(2)RNA干渉(RNAi)法により、これまでにクローン化して来た生体防御関連遺伝子をノックダウン(遺伝子産物発現抑制)し、これら生体防御関連遺伝子の生体内機能の解析を行うとともに、
(3)2本鎖RNA投与による微生物感染に対する感受性変化の検討ならびに耐病性との関係について検討
(4)クルマエビ類の生体防御関連遺伝子発現制御ネットワークの解明

Research Products

• [Journal Article] Uncovering the Mechanisms of Shrimp Innate Immune Response by RNA Interference. (2011)
  • Author(s): Hirono I, Fagutao FF, Kondo H, Aoki T.
  • Journal Title: Mar Biotechnol in press
• [Journal Article] PmPPAE2, a new class of crustacean prophenoloxidase (proPO)-activating enzyme and its role in PO activation. (2011)
  • Author(s): Charoensapsri W, Amparyup P, Hirono I, Aoki T, Tassanakajon A.
  • Journal Title: Dev Comp Immunol. 35 Pages: 115-124
• [Journal Article] A cDNA microarray approach for analyzing transcriptional changes in Penaeus monodon after infection by pathogens. (2010)
  • Author(s): Pongsomboon S, Tang S, Boonda S, Aoki T, Hirono I, Tassanakajon A.
  • Journal Title: Fish Shellfish Immunol. 30 Pages: 439-446
• [Journal Article] The Marsupenaeus japonicus voltage-dependent anion channel (MjVDAC) protein is involved in WSSV pathogenesis. (2010)
  • Author(s): Wang HC, Kondo H, Hirono I, Aoki T.
  • Journal Title: Fish Shellfish Immunol 29 Pages: 94-103
• [Journal Article] Hyper-expansion of large DNA segments in the genome of kuruma shrimp, Marsupenaeus japonicus. (2010)
  • Author(s): Koyama T, Asakawa S, Katagiri T, Shimizu A, Fagutao FF, Mavichak R, Santos MD, Fuji K, Sakamoto T, Kitakado T, Kondo H, Shimizu N, Aoki T, Hirono I.
  • Journal Title: BMC Genomics 11 Pages: 141
• [Journal Article] Molecular characterization and expression analysis of a c-type and two novel muramidase-deficient i-type lysozymes from Penaeus monodon. (2010)
  • Author(s): Supungul P, Rimphanitchayakit V, Aoki T, Hirono I, Tassanakajon A.
  • Journal Title: Fish Shellfish Immunol 28 Pages: 490-498
• [Journal Article] Involvement of WSSV-shrimp homologs in WSSV infectivity in kuruma shrimp ; Marsupenaeus japonicus. (2010)
  • Author(s): Dang LT, Koyama T, Shitara A, Kondo H, Aoki T, Hirono I.
  • Journal Title: Antiviral Res 88 Pages: 217-226
• [Journal Article] Increased bacterial load in shrimp hemolymph in the absence of prophenoloxidase. (2009)
  • Author(s): Fagutao FF, Koyama T, Kaizu A, Saito-Taki T, Kondo H, Aoki T, Hirono I.
  • Journal Title: FEBS J. 276 Pages: 5298-5306
• [Journal Article] Differential gene expression in black tiger shrimp, Penaeus monodon, following administration of oxytetracycline and oxolinic acid. (2009)
  • Author(s): Fagutao FF, Yasuike M, Santos MD, Ruangpan L, Sangrunggruang K, Tassanakajon A, Takahashi Y, Ueno R, Kondo H, Hirono I, Aoki T.
  • Journal Title: Dev Comp Immunol. 33 Pages: 1088-1092
• [Journal Article] Gene expression profile of haemocytes of kuruma shrimp, Marsupenaeus japonicus following peptidoglycan stimulation. (2008)
  • Author(s): Fagutao FF, Yasuike M, Caipang CM, Kondo H, Hirono I, Takahashi Y, Aoki T.
  • Journal Title: Mar Biotechnol 10 Pages: 731-740
• [Journal Article] Differentially expressed genes in Penaeus monodon hemocytes following infection with yellow head virus. (2008)
  • Author(s): Pongsomboon S, Tang S, Boonda S, Aoki T, Hirono I, Yasuike M, Tassanakajon A.
  • Journal Title: BMB Rep. 41 Pages: 670-677
• [Journal Article] A peroxiredoxin from Kuruma shrimp, Marsupenaeus japonicus inhibited by peptidoglycan. (2008)
  • Author(s): Maningas MBB, Koyama T, Kondo H, Hirono I, Aoki T.
  • Journal Title: Dev Comp Immunol. 32 Pages: 198-203
• [Journal Article] Essential function of transglutaminase and clotting protein in shrimp immunity. (2008)
  • Author(s): Maningas MBB, Kondo H, Hirono I, Taki T, Aoki T.
  • Journal Title: Mol.Immunol. 45 Pages: 1269-1275
• [Presentation] RNA干渉法を用いたクルマエビペナエジン様遺伝子の機能解析 (2011)
  • Author(s): 古宮謙太・海津彰弘・近藤秀裕・廣野育生
  • Organizer: 平成23年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学
  • Year and Date: 20110327-20110329
• [Presentation] Molecular immunology on kuruma shrimp, Marsupenaeus japonicus (2010)
  • Author(s): I Hirono, T Koyama, A Kaizu, A Shitara, MBB Maningas, FF Fagtao, H Yamada, K Komiya, T Aoki, H Kondo
  • Organizer: Sixth International Symposium on Aquatic Animal Health
  • Place of Presentation: タンパ、フロリダ
  • Year and Date: 20100905-20100908
• [Presentation] クルマエビリゾチームの生体内での役割について (2010)
  • Author(s): 海津彰弘, 近藤秀裕, 青木宙, 廣野育生
  • Organizer: 第13回マリンバイオテクノロジー学会
  • Place of Presentation: 広島大学
  • Year and Date: 20100529-20100530