Study on shrimp immune system against microbial pathogens

2011 Fiscal Year Final Research Report

• Project/Area Number: 20380109
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General fisheries
• Research Institution: Tokyo University of Marine Science and Technology
• Principal Investigator: HIRONO Ikuo 東京海洋大学, 海洋科学技術研究科, 教授 (00270926)
• Co-Investigator(Kenkyū-buntansha): KONDO Hidehiro 東京海洋大学, 海洋科学技術研究科, 准教授 (20314635)
• Project Period (FY): 2008 – 2011

Research Abstract

We identified that TGase is an important component of the shrimp immune response and is involved in the regulation of some immune-related genes particularly antimicrobial peptides. We also found the potential of non-specific dsRNA injection to shrimp to elicit antimicrobial response in shrimp. We developed the microarray of kuruma shrimp based on the EST analysis for further study of kuruma shrimp immune gene network.

Research Products

• [Journal Article] Uncovering the Mechanisms of Shrimp Innate Immune Response by RNA Interference (2011)
  • Author(s): Hirono I, Fagutao FF, Kondo H, Aoki T
  • Journal Title: Mar Biotechnol Volume: 13 Pages: 622-628
  • DOI: DOI:10.1007/s10126-010-9292-0
• [Journal Article] Molecular characterization and expression analysis of heat shock proteins 40, 70 and 90 from kuruma shrimp Marsupenaeus japonicus (2011)
  • Author(s): Danwattananusorn T, Fagutao FF, Shitara A, Kondo H, A oki T, Nozaki R, Hirono I
  • Journal Title: Fish Sci Volume: 77 Pages: 929-937
  • DOI: DOI:10.1007/s12562-011-0394-z
• [Journal Article] Involvement of WSSV-shrimp homologs in WSSV infectivity in kuruma shrimp ; Marsupenaeus japonicus (2010)
  • Author(s): Dang LT, Koyama T, Shitara A, Kondo H, Aoki T, Hirono I
  • Journal Title: Antiviral Res Volume: 88 Pages: 217-226
  • DOI: DOI:10.1016/j.antiviral.2010.08.017
• [Journal Article] Molecular characterization and expression analysis of a c-type and two novel muramidase-deficient i-type lysozymes from Penaeus monodon (2010)
  • Author(s): Supungul P, Rimphanitchayakit V, Aoki T, Hirono I, Tassanakajon A
  • Journal Title: Fish Shellfish Immunol Volume: 28 Pages: 490-498
  • DOI: DOI:10.1016/j.fsi.2010.01.003
• [Journal Article] Hyper-expansion of large DNA segments in the genome of kuruma shrimp, Marsupenaeus japonicus (2010)
  • Author(s): Koyama T, Asakawa S, Katagiri T, Shimizu A, Fagutao FF, Mavichak R, Santos MD, Fuji K, Sakamoto T, Kitakado T, Kondo H, Shimizu N, Aoki T, Hirono I
  • Journal Title: BMC Genomics Volume: 11 Pages: 141
  • DOI: DOI:10.1186/1471-2164-11-141
• [Journal Article] The Marsupenaeus japonicus voltage-dependent anion channel(MjVDAC) protein is involved in WSSV pathogenesis (2010)
  • Author(s): Wang HC, Kondo H, Hirono I, Aoki T
  • Journal Title: Fish Shellfish Immunol Volume: 29 Pages: 94-103
  • DOI: DOI:10.1016/j.fsi.2010.02.020
• [Journal Article] A cDNA microarray approach for analyzing transcriptional changes in Penaeus monodon after infection by pathogens (2010)
  • Author(s): Pongsomboon S, Tang S, Boonda S, Aoki T, Hirono I, Tassanakajon A
  • Journal Title: Fish Shellfish Immunol Volume: 30 Pages: 439-446
  • DOI: DOI:10.1016/j.fsi.2010.10.015
• [Journal Article] Increased bacterial load in shrimp hemolymph in the absence of prophenoloxidase (2009)
  • Author(s): Fagutao FF, Koyama T, Kaizu A, Saito-Taki T, Kondo H, Aoki T, Hirono I
  • Journal Title: FEBS J Volume: 276 Pages: 5298-5306
  • DOI: DOI:10.1111/j.1742-4658.2009.07225.x
• [Journal Article] Differential gene expression in black tiger shrimp, Penaeus monodon, following administration of oxytetracycline and oxolinic acid (2009)
  • Author(s): Fagutao FF, Yasuike M, Santos MD, Ruangpan L, Sangrunggruang K, Tassanakajon A, Takahashi Y, Ueno R, Kondo H, Hirono I, Aoki T
  • Journal Title: Dev Comp Immunol Volume: 33 Pages: 1088-1092
  • DOI: DOI:10.1016/j.dci.2009.05.010
• [Journal Article] Gene expression profile of haemocytes of kuruma shrimp, Marsupenaeus japonicus following peptidoglycan stimulation (2008)
  • Author(s): Fagutao FF, Yasuike M, Caipang CM, Kondo H, Hirono I, Takahashi Y, Aoki T
  • Journal Title: Mar Biotechnol Volume: 10 Pages: 731-740
  • DOI: DOI:10.1007/s10126-008-9110-0
• [Presentation] Molecular immunology on kuruma shrimp (2010)
  • Author(s): I Hirono, T Koyama, A Kaizu, A Shitara, MBB Maningas, FF Fagtao, H Yamada, K Komiya, T Aoki, H Kondo
  • Organizer: Marsupenaeus japonicus, Sixth International Symposium on Aquatic Animal Health
  • Place of Presentation: タンパマリオットウオーターサイドホテル(タンパ、フロリダ)
  • Year and Date: 20100906-08
• [Presentation] Shrimp lysozyme is important to regulate gram-negative bacteria in haemolymph (2010)
  • Author(s): A Kaizu, H Kondo, T Aoki and I Hirono
  • Organizer: Sixth International Symposium on Aquatic Animal Health
  • Place of Presentation: タンパマリオットウオーターサイドホテル(タンパ、フロリダ)
  • Year and Date: 20100906-08
• [Presentation] クルマエビのペナエジン様cDNA構造およびRNA干渉による機能解析 (2010)
  • Author(s): 古宮謙太・海津彰弘・近藤秀裕・廣野育生
  • Organizer: 平成22年度日本水産学会秋期大会
  • Place of Presentation: 京都大学(京都府)
  • Year and Date: 2010-09-22
• [Presentation] Molecular biology of shrimp (2010)
  • Author(s): Ikuo Hirono
  • Organizer: 2010 Symposium on Biotechnology Development in Taiwan
  • Place of Presentation: National Cheng Kung University(台南市、台湾)
  • Year and Date: 2010-04-20
• [Presentation] Molecular Immunology and Genomics of Shrimp, ABIC 2009 (2009)
  • Author(s): Ikuo Hirono
  • Organizer: Agricultural Biotechnology for Better Living and a Clean Environment
  • Place of Presentation: Queen Sirikit National Convention Center, Bangkok, Thailand
  • Year and Date: 20090922-25