Trypsin is a multifunctional factor in spermatogenesis and male reproduction in fish.

2011 Fiscal Year Final Research Report

• Project/Area Number: 20380112
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General fisheries
• Research Institution: Ehime University
• Principal Investigator: MIURA Takeshi 愛媛大学, 南予水産研究センター, 教授 (00261339)
• Co-Investigator(Kenkyū-buntansha): KOBAYASHI Touru 静岡県立大学, 環境科学研究所, 教授 (30221972)
• Project Period (FY): 2008 – 2011
• Keywords: 生殖

Research Abstract

Trypsin is well known as a pancreatic enzyme that is typically secreted into the intestine to digest proteins. In this study, I provide strong evidence that the testicular trypsins are important factors in the control of meiosis, spermiogenesis and fertilization using fish models. In other words, trypsin is a multifunctional factor in male reproduction.

Research Products

• [Journal Article] The role of taurine in the eel spermatogenesis (2011)
  • Author(s): Higuchi, M., Celino.F. T., Miura, C. and Miura, T.
  • Journal Title: Proceedings of YSEPE2011 Volume: (in press)
• [Journal Article] Tolerance of spermatogonia to oxidative stress is due to high levels of Zn and Cu/Zn superoxide dismutase (2011)
  • Author(s): Celino F. T., Yamaguchi, S., Miura, C., Ohta, T., Tozawa, Y., Iwai, T. and Miura, T.
  • Journal Title: Plos One Volume: 6(2) Pages: 16938
• [Journal Article] Differences of two polychaete species reflected in enzyme activities (2011)
  • Author(s): Ito, K., Nozaki, M., Ohta, T., Miura, C., Tozawa, Y. and Miura, T.
  • Journal Title: Marine Biology Volume: 150 Pages: 1211-1221
• [Journal Article] Anti-viral effects of interferon administration on seven-band grouper, Epinephelus septemfasciatus (2011)
  • Author(s): Ohta, T., Ueda, Y. Ito, K., Miura, C., Yamashita, H., Miura, T. and Tozawa, Y.
  • Journal Title: Fish & Shellfish Immunology Volume: 30 Pages: 1064-1071
• [Journal Article] Analysis of spermatogenesis using an eel model (2011)
  • Author(s): Miura, C., Miura, T.
  • Journal Title: Aqua-Bio Science Monographs Volume: 4 Pages: 105-129
• [Journal Article] Growth hormone is produced by the testis of Japanese eel and stimulates proliferation of spermatogonia (2011)
  • Author(s): Miura, C., Shimizu, Y., Uehara, M., Ozaki, Y., Young, G. and Miura, T.
  • Journal Title: Reproduction Volume: 142 Pages: 869-877
• [Journal Article] マダイの成長と免疫系に及ぼすオニヒトデの効果 (2011)
  • Author(s): 三浦智恵美・三浦猛
  • Journal Title: 養殖 Volume: 48(2) Pages: 44-47
• [Journal Article] 技術.養殖ブリの産卵リスクとその制御のための給餌管理-制限給餌による成熟抑制と成長遅滞の解消- (2011)
  • Author(s): 三浦猛・三浦智恵美・三浦仁嗣
  • Journal Title: アクアネット Volume: 14(1) Pages: 52-59
• [Journal Article] "嫌われ者"オニヒトデの意外な利用法 (2010)
  • Author(s): 三浦猛
  • Journal Title: アクアネット Volume: 13(3) Pages: 25
• [Journal Article] 魚粉代替飼料減量としてのイエバエ幼虫とサナギの有効性 (2010)
  • Author(s): 三浦猛
  • Journal Title: 養殖 Volume: 47(3) Pages: 44-46
• [Journal Article] Spermatogenesis in fish (2010)
  • Author(s): Schulz, R., Luiz, F., Jean-Jacques L., LeGac F., Chiarini-Garcia F., Nobrega, H. and Miura, T.
  • Journal Title: General and Comparative Endocrinology Volume: 165 Pages: 390-411
• [Journal Article] Study of sediment cleanup using polychaete (2010)
  • Author(s): Ito, K., Nozaki, N., Kunihiro, T., Miura, C. and Miura, T.
  • Journal Title: International Symposium on Modeling and Analysis of Marine Environmental Problems(MAMEP Sympo. 2010) Pages: 133-139
• [Journal Article] The influence of water pollution on reproduction of aquatic animals living in the Indochina Peninsula. (2009)
  • Author(s): Yamaguchi, S., Miura, C. and Miura, T.
  • Journal Title: Chemical Pollution in Indochina Pages: 113-126
• [Journal Article] Trypsin is a multifunctional factor in spermatogenesis (2009)
  • Author(s): Miura, C., Ohta, T., Ozaki, Y., Tanaka, H. and Miura, T.
  • Journal Title: PNAS Volume: 106 Pages: 20972-20977
• [Journal Article] Arsenic inhibits in vitro spermatogenesis and induces germ cell apoptosis in Japanese eel(Anguilla japonica) (2009)
  • Author(s): Celino, F. T., Yamaguchi, S., Miura, C., and Miura, T.
  • Journal Title: Reproduction Volume: 138 Pages: 279-287
• [Journal Article] Zinc is an essential trace element for spermatogenesis (2009)
  • Author(s): Yamaguchi, S., Miura C., Kikuchi, K., Celino, F. T., Agusa, T., Tanabe, S., and Miura, T.
  • Journal Title: PNAS Volume: 106 Pages: 10859-10864
• [Journal Article] The contribution of endogenous cellulase to cellulose digestion in the gut of earthworm (2009)
  • Author(s): Nozaki, M., Miura, C., Tozawa, Y. and Miura, T.
  • Journal Title: Soil Biol. Biochem Volume: 41 Pages: 762-769
• [Journal Article] 環境化学物質と魚介類の生殖 (2009)
  • Author(s): 三浦猛
  • Journal Title: 分子でよむ環境汚染 Pages: 102-121
• [Journal Article] Progestin is an essential factor for the initiation of the meiosis in spermatogenesis and early oogenesis in fish (2008)
  • Author(s): Miuta, T. and Miura, C.
  • Journal Title: Cybium Volume: 32(2) Pages: 130-132
• [Journal Article] 17α, 20β-dihydroxy-4-pregnen-3-one induced substances, trypsin and 11β-hydroxysteroid dehydrogenase, are important factors for control of spermatogenesis in Japanese eel (2008)
  • Author(s): Miura, C., Ozaki, Y., Higuchi, M., Ohta, T. and Miura, T.
  • Journal Title: Cybium Volume: 32(2) Pages: 164
• [Journal Article] The role of trypsin-like protease in sperm on fertilization in fish (2008)
  • Author(s): Ohta, T., Miura, C., Shimizu, Y., Mizuno, K., Matsubara, H., Tanaka, H. and Miura, T.
  • Journal Title: Cybium Volume: 32(2) Pages: 166
• [Journal Article] Effects of trace elements on spermatogenesis and androgen production in fish (2008)
  • Author(s): Yamaguchi, S., Miura, C., Celino, F. T., and Miura, T.
  • Journal Title: Cybium Volume: 32(2) Pages: 165
• [Journal Article] Testicular Toxicity of Arsenic on Spermatogenesis in Fish. Proceedings of the International Symposium on Biological Responses to Chemical Pollutants (2008)
  • Author(s): Celino, F. T., Yamaguchi, S., Miura, C. and Miura T.
  • Journal Title: Interdisciplinary Studies on Environmental Chemistry, Ehime, March 2007 Pages: 55-60
• [Journal Article] The role of the earthworm, Pheretima(Metaphire) hilgendorfi, in terrestrial ecosystem nutrient cycling. (2008)
  • Author(s): Nozaki, M., Miura, C., Tozawa, Y. and Miura, T.
  • Journal Title: Proceedings of the International Symposium on Biological Responses to Chemical Pollutants : Interdisciplinary Studies on Environmental Chemistry, Ehime, March 2007 Pages: 275-279
• [Journal Article] Effects of arsenic on gonadal development in freshwater crab, Somanniathelphusa pax, in Vietnam and Geothelphusa dehaani in Japan (2008)
  • Author(s): Yamaguchi, S., Celino, F. T., Ito, A., Agusa, T., Tanabe, S., Tuyen, B. C., Miura, C. and Miura, T.
  • Journal Title: Ecotoxicology Volume: 17 Pages: 772-780
• [Journal Article] Japanese Eel Follicle-Stimulating Hormone(Fsh) and Luteinizing Hormone(Lh): Production of Biologically Active Recombinant Fsh and Lh by Drosophila S2 Cells and Their Differential Actions on the Reproductive Biology (2008)
  • Author(s): Kazeto Y., Kohara M., Miura T., Miura C., Yamaguchi S., Trant J. M., Adachi S. and Yamauchi, K.
  • Journal Title: Biol. Reprod Volume: 79 Pages: 938-946
• [Journal Article] The synthesis and role of taurine in the Japanese eel testis
  • Author(s): Higuchi, M., Celino, F. T., Tamai, A., Miura, C. and Miura, T.
  • Journal Title: Amino Acids Volume: (in press)
  • DOI: DOI:10.1007/s00726-011-1128-3
• [Journal Article] Molecular characterization and gene expression of Japanese eel(Anguilla japonica) gonadotropin receptors
  • Author(s): Kazeto, Y., Koyama, M., Tosaka, R., Gen, K., Yokoyama, M., Miura, C., Miura, T., Adachi, S. and Yamauchi, K.
  • Journal Title: Zoological. Science Volume: (in press)
• [Presentation] Recent research trend for the culture of pearls in Uwa sea, Ehime (2011)
  • Author(s): Miura, T.
  • Organizer: The international Symposium on Pearl Research
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 20111000
• [Presentation] The induction of sterility using RNA interference technique in Pinctada fucata martensi (2011)
  • Author(s): Watanabe, N., Celino.F. T., Miura, C., Ohashi, H., Fukushima, E., Iwai, T., Hosokawa, H., Uchimura, Y. and Miura, T.
  • Organizer: The international Symposium on Pearl Research
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 20111000
• [Presentation] Selective bleeding of disease-resistant Japanese pearl oysters using molecular markers (2011)
  • Author(s): Sone, K., Iwai, T., Uehara, M., Watanabe, N., Celino.F. T., Hosokawa, H., Odawara, K., Miura, C. and Miura, T.
  • Organizer: The international Symposium on Pearl Research
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 20111000
• [Presentation] Newly developed technique of producing pearl using mantle fragment of Pinctada margaritifera and Pteria penguin in hyposensitized Akoya pearl oyster, Pinctada fucata (2011)
  • Author(s): Fukushima, E., Iwai, T., Miura, C., Watanabe, N., Urasaki, S., Kuwayama, K., Odawara, K. and Miura, T.
  • Organizer: The international Symposium on Pearl Research
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 20111000
• [Presentation] Synthesis of taurine and its role in Japanese eel spermatogenesis (2011)
  • Author(s): Higuchi, M., Miura, C. and Miura, T.
  • Organizer: World Congress on Reproductive Biology
  • Place of Presentation: Cairns, Australia
  • Year and Date: 20111000
• [Presentation] Growth hormone in synthesis in the testis and stimulates spermatogonial proliferation in Japanese eel (2011)
  • Author(s): Miura, C., Shimizu, Y., Uehara, M., Ozaki, Y., Young, G. and Miura, T.
  • Organizer: Anguilla japonica World Congress on Reproductive Biology
  • Place of Presentation: Cairns, Australia
  • Year and Date: 20111000
• [Presentation] Gonad in one of the control organs for growth in fish (2011)
  • Author(s): Bhatta, S., Iwai, T., Miura, C., Higuchi, M., Yamaguchi, S. and Miura, T.
  • Organizer: World Congress on Reproductive Biology
  • Place of Presentation: Cairns, Australia
  • Year and Date: 20111000
• [Presentation] 生殖腺は魚類の成長をコントロールする (2011)
  • Author(s): Sandip Bhatta. 岩井俊治. 三浦智恵美. 樋口理人. 清水園子. 三浦猛
  • Organizer: 平成23年度日本水産学会秋季大会
  • Place of Presentation: 長崎市
  • Year and Date: 20110900-1000
• [Presentation] ティラピアの成長の雌雄差と生殖腺の発達の関係について (2011)
  • Author(s): Sandip Bhatta. 樋口理人. 岩井俊治. 清水園子. 三浦智恵美. 三浦猛
  • Organizer: 平成23年度日本水産学会秋季大会
  • Place of Presentation: 長崎市
  • Year and Date: 20110900-1000
• [Presentation] タウリンがウナギ精子形成の及ぼす作用1 (2011)
  • Author(s): 樋口理人. 三浦智恵美. 三浦猛
  • Organizer: 平成23年度日本水産学会秋季大会
  • Place of Presentation: 長崎市
  • Year and Date: 20110900-1000
• [Presentation] タウリンがウナギ精子形成の及ぼす作用2 (2011)
  • Author(s): 樋口理人. 三浦智恵美. 三浦猛
  • Organizer: 平成23年度日本水産学会秋季大会
  • Place of Presentation: 長崎市
  • Year and Date: 20110900-1000
• [Presentation] タウリンがウナギ精子形成の及ぼす作用3 (2011)
  • Author(s): 樋口理人. 三浦智恵美. 三浦猛
  • Organizer: 平成23年度日本水産学会秋季大会
  • Place of Presentation: 長崎市
  • Year and Date: 20110900-1000
• [Presentation] Effect of taurine on eel spermatogenesis (2011)
  • Author(s): Higuchi, M., Celino.F. T., Miura, C. and Miura, T.
  • Organizer: International symposium on Advanced Studies by Young Scientists on Environmental Pollution and Ecotoxicology
  • Place of Presentation: Matsuyama, Japan
  • Year and Date: 20110800
• [Presentation] 魚類の成長を直接コントロールする器官としての生殖腺 (2011)
  • Author(s): Sandip Bhatta.岩井俊治.樋口理人.山口園子.三浦智恵美.三浦猛
  • Organizer: 平成23年度日本水産学会春季大会
  • Place of Presentation: 東京都
  • Year and Date: 20110300
• [Presentation] ニホンウナギ精巣でのタウリン合成機構 (2011)
  • Author(s): 樋口理人. Celino.F. T. 玉井綾子. 山口園子. 三浦智恵美. 三浦猛
  • Organizer: 平成23年度日本水産学会春季大会
  • Place of Presentation: 東京都
  • Year and Date: 20110300
• [Presentation] オニヒトデのマダイ成長と免疫系に及ぼす効果 (2010)
  • Author(s): 三浦智恵美・伊藤克敏・上田祐輔・太田加代子・岩井俊治・太田耕平・浦崎慎太郎・山本正文・三浦猛
  • Organizer: 平成22年度日本水産学会秋季大会
  • Place of Presentation: 京都
  • Year and Date: 20100900
• [Presentation] 制限給餌による養殖ブリの成熟・成長コントロール (2010)
  • Author(s): 吉原勇作・三浦智恵美・山口園子・樋口理人・竹田豊・浜田克人・三浦仁嗣・三浦猛
  • Organizer: 平成22年度日本水産学会秋季大会
  • Place of Presentation: 京都
  • Year and Date: 20100900
• [Presentation] イエバエ由来物の魚類成長および免疫系への作用 (2010)
  • Author(s): 岩井俊治・伊藤克敏・上田祐輔・三浦智恵美・浦崎慎太郎・太田加代子・山口園子・串間充崇・三浦猛
  • Organizer: 平成22年度日本水産学会秋季大会
  • Place of Presentation: 京都
  • Year and Date: 20100900
• [Presentation] 酸化ストレスに対する魚類精原細胞の防御機構 (2010)
  • Author(s): 山口園子・Fritze T. Celino・三浦智恵美・三浦猛
  • Organizer: 平成22年度日本水産学会秋季大会
  • Place of Presentation: 京都
  • Year and Date: 20100900
• [Presentation] マハタ組み換えインターフェロンの効果 (2010)
  • Author(s): 上田祐輔・太田史・伊藤克敏・岩井俊治・浦崎慎太郎・山下浩史・戸澤譲・三浦智恵美・三浦猛
  • Organizer: 平成22年度日本水産学会秋季大会
  • Place of Presentation: 京都
  • Year and Date: 20100900
• [Presentation] 亜鉛は精子形成にとって必須元素 (2009)
  • Author(s): 三浦猛
  • Organizer: 日本動物学会北海道支部第532回支部講演会
  • Place of Presentation: 札幌
  • Year and Date: 20091100
• [Presentation] ニホンウナギ精巣における抗酸化機構 (2009)
  • Author(s): 山口園子・Celino, F. T.・三浦智恵美・太田史・戸澤譲・三浦猛
  • Organizer: 平成21年度日本水産学会春季大会
  • Place of Presentation: 東京
  • Year and Date: 20090400
• [Presentation] 成長ホルモンのウナギ精巣への作用に関する研究 (2009)
  • Author(s): 上原真保・清水陽介・三浦智恵美・三浦猛
  • Organizer: 平成21年度日本水産学会春季大会
  • Place of Presentation: 東京
  • Year and Date: 20090400
• [Presentation] ニホンウナギ精子形成でのトリプシン関連因子の発現解析 (2009)
  • Author(s): 三浦智恵美・太田史・三浦猛
  • Organizer: 平成21年度日本水産学会春季大会
  • Place of Presentation: 東京
  • Year and Date: 20090300
• [Presentation] Impact of oxidative stress on in vitro Japanese eel spermatogenesis (2009)
  • Author(s): Celino, F. T., Yamaguchi, S., Miura, C. and Miura, T.
  • Organizer: 平成21年度日本水産学会春季大会
  • Place of Presentation: 東京
  • Year and Date: 20090300
• [Presentation] Male reproductive toxicity of arsenic in fish : an in vitro study using eel culture system (2008)
  • Author(s): Celino, F. T., Yamaguchi, S., Miura, C. and Miura, T.
  • Place of Presentation: Calgary, Canada
  • Year and Date: 20080600
• [Presentation] Testicular trypsins are important substances for the control of initiation of meiosis, spermiogenesis and fertilization (2008)
  • Author(s): Miura, C., Ohta, T. and Miura, T.
  • Organizer: An international symposium in honor of Professor Nagahama, Y.: Sex determination and gametogenesis in fish : current status and future directions
  • Place of Presentation: Hawaii, USA
  • Year and Date: 20080500
• [Presentation] Study on the role of GH and GH-related proteins in spermatogenesis in Japanese eel (2008)
  • Author(s): Maugars, G., Shimizu, Y., Uehara, M., Miura, C. and Miura, T.
  • Organizer: An international symposium in honor of Professor Nagahama, Y.: Sex determination and gametogenesis in fish : current status and future directions
  • Place of Presentation: Hawaii, USA
  • Year and Date: 20080500
• [Presentation] Effects of zinc on spermatogenesis in Japanese eel(Anguilla Japonica) (2008)
  • Author(s): Yamaguchi, S., Miura, C., Celino, F. T., Agusa, T. and Miura, T.
  • Organizer: An international symposium in honor of Professor Nagahama, Y.: Sex determination and gametogenesis in fish : current status and future directions
  • Place of Presentation: Hawaii, USA
  • Year and Date: 20080500
• [Presentation] Analysis of the direct influence of arsenic on fish spermatogenesis using eel testicular culture system (2008)
  • Author(s): Celino, F. T., Yamaguchi, S., Miura, C. and Miura, T.
  • Organizer: An international symposium in honor of Professor Nagahama, Y.: Sex determination and gametogenesis in fish : current status and future directions
  • Place of Presentation: Hawaii, USA
  • Year and Date: 20080500