Overcoming of problem in direct stock assessment by egg production method and its application in the chub mackerel as model species

2010 Fiscal Year Final Research Report

• Project/Area Number: 20380113
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General fisheries
• Research Institution: Kyushu University
• Principal Investigator: MATSUYAMA Michiya Kyushu University, 大学院・農学研究院, 教授 (00183955)
• Co-Investigator(Kenkyū-buntansha): YAMAGUCHI Akihiko 九州大学, 大学院・農学研究院, 助教 (10332842)
• Project Period (FY): 2008 – 2010
• Keywords: 卵数法 / 資源量推定 / 産卵数 / 産卵頻度 / マサバ

Research Abstract

To develop and establish the direct stock assessment by the Egg Production Method in chub mackerel, following studies have been performed. 1) Development of the new clearing solution for identification of the nucleus in the formalin-preserved oocyte, 2) Development of the simple method to identify the postovulatory follicles by using PAS staining, and estimation of the spawning frequency in the wild stock, 3) Analysis of the cell division cycles in oogonia and oocytes, and mode of the determination of total fecundity, and 4) Evaluation of spawning in individual female fish based on the mitochondrial DNA analysis.

Research Products

• [Journal Article] Molecular characteri-zation, tissue distribution, and mRNA expression profiles of two Kiss genes in the adult male and female chub mackerel (Scomber japonicus) during different gonadal stages. (2010)
  • Author(s): Selvaraj S, Kitano H, Fujinaga Y, Ohga H, Yoneda M, Yamaguchi A, Shimizu A, Matsuyama M
  • Journal Title: Gen.Comp.Endocrinol. 169 Pages: 28-38
  • Peer Reviewed
• [Journal Article] 産卵親魚個体群の繁殖能力と加入量変動-母性効果研究の現状と今後の展開- (2010)
  • Author(s): 栗田豊, 米田道夫, 松山倫也
  • Journal Title: 水産海洋研究 74特集号 Pages: 1-3
  • Peer Reviewed
• [Journal Article] 産魚類の生殖周期の内分泌制御機構 (2010)
  • Author(s): 松山倫也
  • Journal Title: 水産海洋研究 74特集号 Pages: 66-83
  • Peer Reviewed
• [Journal Article] Immunological characterization and distribution of three GnRH forms in the brain and pituitary gland of chub mackerel (Scomber japonicus). (2009)
  • Author(s): Selvaraj S, Kitano H, Fujinaga Y, Amano M, Takahashi A, Shimizu A, Yoneda M,
  • Journal Title: Zool.Sci. 26 Pages: 828-839
  • Peer Reviewed
• [Journal Article] Estimation of the daily food intake and gross growth efficiency of chub mackerel (Scomber japonicas) larvae under various temperatures. (2009)
  • Journal Title: Aquaculture Science 57 Pages: 291-299
  • Peer Reviewed
• [Journal Article] Spawning frequency of the Tsushima Current subpopulation of chub mackerel Scomber japonicus off Kyushu, Japan. (2009)
  • Author(s): Yamaguchi A, Matsuyama M
  • Journal Title: Fisheries Science 75 Pages: 649-655
  • Peer Reviewed
• [Journal Article] Time course of final oocyte maturation and ovulation in chub mackerel Scomber japonicus induced by hCG and GnRHa. (2009)
  • Author(s): Mendiola D, Yamashita Y, Matsuyama M, Masuda R, Okamoto K, Alvarez P, Tanaka M.
  • Journal Title: Fisheries Science 74 Pages: 764-769
  • Peer Reviewed
• [Journal Article] 水産重要魚種における配偶子形成の内分泌機構-サバ等- (2009)
  • Author(s): Shiraishi T, Ketkar SD, Katoh Y, Nyuji M, Yamaguchi A,, Matsuyama M
  • Journal Title: 日本水産学会誌 75 Pages: 864-865
• [Journal Article] Age validation, growth and annual reproductive cycle of chub mackerel, Scomber japonicus, off the waters of northern Kyushu and in the East China Sea. (2008)
  • Author(s): Shiraishi T, Ketkar SD, Kitano H, Nyuji M, Yamaguchi A,, Matsuyama M
  • Journal Title: Eisheries Science 74 Pages: 947-954
  • Peer Reviewed
• [Journal Article] Scomber japonicus, H. is a better candidate species for juvenile production activities than Scomber scombrus (2008)
  • Author(s): 松山倫也
  • Journal Title: L.Aquaculture Research 39 Pages: 1122-1127
  • Peer Reviewed
• [Journal Article] 魚類の生殖生理学的研究と水産増養殖・資源管理への応用 (2008)
  • Author(s): Shiraishi T, Okamoto K, Yoneda M, Sakai T, Ohshimo S, Onoe S, Yamaguchi A, Matsuyama M
  • Journal Title: 日本水産学会誌 74 Pages: 371-374
  • Peer Reviewed
• [Presentation] 小型浮魚3種の産卵誘導法の特徴 (2010)
  • Author(s): 松山倫也, 北野載, 米田道夫, 白石哲朗, Selvaraj S, 入路光雄, 大賀浩史, 藤澤和輝, 川村耕平, 山口明彦, 清水昭男, 田中寛繁, 大下誠二
  • Organizer: 2010年度水産海洋学会研究発表大会,38
  • Place of Presentation: 東京
  • Year and Date: 2010-11-20
• [Presentation] マサバ2歳魚の卵サイズの変化が仔魚の成長と生残に及ぼす影響 (2010)
  • Author(s): 米田道夫, 北野載, Selvaraj S, 入路光雄, 川村耕平, 松山倫也, 清水昭男
  • Organizer: 2010年度水産海洋学会研究発表大会,39
  • Place of Presentation: 東京
  • Year and Date: 2010-11-20
• [Presentation] 水温がカタクチイワシの卵黄形成速度に及ぼす影響と産卵間隔との関係 (2010)
  • Author(s): 北野載, 米田道夫, 川村耕平, 入路光雄, Sethu Selvaraj, 田中寛繁, 大下誠二. 清水昭男, 松山倫也
  • Organizer: 2010年度水産海洋学会研究発表大会,P05
  • Place of Presentation: 東京
  • Year and Date: 2010-11-20
• [Presentation] 免疫組織化学的手法に基づく産卵周期におけるマサバ脳下垂体GtH産生細胞の変化 (2010)
  • Author(s): 入路光雄, 金子賢介, 北野載, 山口明彦, 清水昭男, 松山倫也
  • Organizer: 日本水産増殖学会第9回大会,11
  • Place of Presentation: 唐津
  • Year and Date: 2010-10-30
• [Presentation] 卵成熟誘起能とステロイド産生能から推定したマサバの2種生殖腺刺激ホルモン(GtH)の機能 (2010)
  • Author(s): 大賀浩史, 入路光雄, 北野載, S Selvaraj, 金子賢介, 清水昭男, 山口明彦, 松山倫也
  • Organizer: 日本水産増殖学会第9回大会,25
  • Place of Presentation: 唐津
  • Year and Date: 2010-10-30
• [Presentation] Source and suitability of adult jack mackerel (Trachurus japonicus) for induction of maturation and spawning. (2010)
  • Author(s): Nyuji M, Fujisawa K, Kitano H, Yoda M, Yoneda M, Shimizu A, Yamaguchi A, Matsuyama M
  • Organizer: UJNR Aquaculture Panel 39th Scientific Symposium "The Present and Future of the Aquaculture Industry" 63
  • Place of Presentation: Kagoshima, Japan
  • Year and Date: 2010-10-25
• [Presentation] Captive reproduction of chub mackerel : experimental system and analysis of neuroendocrine hormones. (2010)
  • Author(s): Selvaraj S, Shiraishi T, Kitano H, Amano M, Nyuji M, Ohga H, Yamaguchi A, Yoneda M, Shimizu A, Matsuyama M
  • Organizer: UJNR Aquaculture Panel 39th Scientific Symposium "The Present and Future of the Aquaculture Industry" 64-66, 25
  • Place of Presentation: Kagoshima, Japan
  • Year and Date: 2010-10-25
• [Presentation] Induction of maturation and spawning of jack mackerel (Trachurus japonicas) temporarily reared in captivity using GnRHa. (2010)
  • Author(s): Nyuji M, Fujisawa K, Kitano H, Yamaguchi A, Yoneda M, Shimizu A, Matsuyama M
  • Organizer: Global Conference on Aquaculture 2010 "Farming the Waters for People and Food", P-025
  • Place of Presentation: Phuket, Thailand.
  • Year and Date: 2010-09-25
• [Presentation] Expression profiles of three GnRH forms in the brain and pituitary of chub mackerel (Scomber japonicus) during ovarian cycle in captivity. (2010)
  • Author(s): Selvaraj S, Kitano H, Amano M, Yamaguchi A, Matsuyama M
  • Organizer: Global Conference on Aquaculture 2010 "Farming the Waters for People and Food", P-028
  • Place of Presentation: Phuket, Thailand.
  • Year and Date: 2010-09-23
• [Presentation] 岩礁域への移行前後のマアジにおける異なる刺激に対する学習能力 (2010)
  • Author(s): 高橋宏司, 益田玲爾, 松山倫也, 山下洋
  • Organizer: 日本動物心理学会第70回大会,27
  • Place of Presentation: 八王子
  • Year and Date: 2010-08-27
• [Presentation] カタクチイワシの初期生残に及ぼす母性効果 (2010)
  • Author(s): 米田道夫, 清水昭男, 北野載, 松山倫也, 田中寛繁, 大下誠二
  • Organizer: 水産海洋学会シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2010-03-30
• [Presentation] 浮標物を条件刺激としたマアジ仔稚魚の学習能力の個体発生 (2010)
  • Author(s): 高橋宏司, 益田玲爾, 山下洋, 松山倫也
  • Organizer: 平成22年度日本水産学会春季大会
  • Place of Presentation: 藤澤
  • Year and Date: 2010-03-29
• [Presentation] マサバのKiSS遺伝子のクローニングおよび生殖周期におけるmRNAの発現量変化 (2010)
  • Author(s): Sethu Selvaraj, 北野載, 山口明彦, 米田道夫, 清水昭男, 松山倫也
  • Organizer: 平成22年度日本水産学会春季大会,311
  • Place of Presentation: 藤澤
  • Year and Date: 2010-03-28
• [Presentation] Identification of two kisspeptin genes in the chub mackerel, Scomber japonicus : molecular cloning and tissue distribution. (2010)
  • Author(s): Selvaraj S, Kitano H, Yamaguchi A, Matsuyama M
  • Organizer: 平成21年度日本水産学会九州支部大会,P.7
  • Place of Presentation: 長崎
  • Year and Date: 2010-01-23
• [Presentation] 飼育下におけるマアジの産卵生態 (2009)
  • Author(s): 藤澤和輝, 北野載, 米田道夫, 清水昭男, 山口明彦, 松山倫也
  • Organizer: 2009年度水産海洋学会研究発表大会,27
  • Place of Presentation: 長崎
  • Year and Date: 2009-11-18
• [Presentation] カタクチイワシの成熟・産卵に及ぼす水温と給餌量の影響 (2009)
  • Author(s): 米田道夫, 北野載, S.Selvaraj, 松山倫也, 清水昭男
  • Organizer: 2009年度水産海洋学会研究発表大会,P11
  • Place of Presentation: 長崎
  • Year and Date: 2009-11-18
• [Presentation] カタクチイワシEngraulis japonicusは毎日産卵が可能か (2009)
  • Author(s): 北野載, 米田道夫, Sethu Selvaraj, 田中寛繁, 大下誠二, 清水昭男, 山口明彦, 松山倫也
  • Organizer: 2009年度水産海洋学会研究発表大会,24
  • Place of Presentation: 長崎
  • Year and Date: 2009-11-18
• [Presentation] BrdU投与によるマサバの生殖細胞における増殖および卵黄蓄積速度の解析 (2009)
  • Author(s): 北野載, 金子賢介, Sethu Selvaraj, 藤永陽一郎, 松藤由佳, 米田道夫, 清水昭男, 山口明彦, 松山倫也
  • Organizer: 水産増殖学会第8回大会,22
  • Place of Presentation: 下関
  • Year and Date: 2009-10-31
• [Presentation] ミトコンドリアDNAを用いた母子判定に基づくマサバの産卵頻度 (2009)
  • Author(s): 松藤由佳, 入路光雄, 金子賢介, 北野載, 山口明彦, 望岡典隆, 松山倫也
  • Organizer: 水産増殖学会第8回大会,23
  • Place of Presentation: 下関
  • Year and Date: 2009-10-31
• [Presentation] カタクチイワシの産卵時刻と配偶子の形成速度に及ぼす水温の影響 (2009)
  • Author(s): 北野載, 米田道夫, Sethu Selvaraj, 田中寛繁, 大下誠二, 清水昭男, 山口明彦, 松山倫也
  • Organizer: 平成21年度日本水産学会秋季大会,229
  • Place of Presentation: 盛岡
  • Year and Date: 2009-10-29
• [Presentation] 水産重要魚種における配偶子形成の内分泌機構.海産魚類(サバ等) (2009)
  • Author(s): 松山倫也
  • Organizer: 平成21年度日本水産学会春季大会シンポジウム「魚類の生殖機構-基礎と応用」
  • Place of Presentation: 東京
  • Year and Date: 2009-03-31
• [Presentation] カタクチイワシの卵巣成熟、産卵に伴う卵巣重量の日周変動 (2009)
  • Author(s): 米田道夫, 北野載, S.Selvaraj, 松山倫也, 清水昭男
  • Organizer: 平成21年度日本水産学会春季大会,457
  • Place of Presentation: 東京
  • Year and Date: 2009-03-30
• [Presentation] マサバの精製GtHによる各種卵濾胞のin vitroステロイド産生能 (2009)
  • Author(s): 金子賢介, 北野載, 清水昭男, 米田道夫, 松藤由佳, S.Selvaraj, 藤永陽一郎, 山口明彦, 松山倫也
  • Organizer: 平成21年度日本水産学会春季大会,305
  • Place of Presentation: 東京
  • Year and Date: 2009-03-28
• [Presentation] hCGによる天然マアジの成熟・排卵誘導とそのタイムコース (2008)
  • Author(s): 松藤由佳, 金子賢介, 北野載, S.Selvaraj, 藤永陽一郎, 米田道夫, 清水昭男, 山口明彦, 松山倫也
  • Organizer: 平成20年度水産海洋学会,58
  • Place of Presentation: 東京
  • Year and Date: 2008-11-13
• [Presentation] マサバの産卵数は産卵期前に決まっているか?-BrdUを用いた生殖細胞の増殖解析- (2008)
  • Author(s): 北野載, 金子賢介, S.Selvaraj, 藤永陽一郎, 松藤由佳, 米田道夫, 清水昭男, 山口明彦, 松山倫也
  • Organizer: 平成20年度水産海洋学会,59
  • Place of Presentation: 東京
  • Year and Date: 2008-11-13
• [Presentation] 給餌条件の向上に伴うカタクチイワシの成長、成熟・産卵の応答 (2008)
  • Author(s): 米田道夫, 北野載, S.Selvaraj, 松山倫也, 清水昭男
  • Organizer: 平成20年度水産海洋学会,60
  • Place of Presentation: 東京
  • Year and Date: 2008-11-13
• [Presentation] Changes in the activities of two types of gonadotropin (GtH) cells during annual and spawning cycle in chub mackerel (Scomber japonicus). (2008)
  • Author(s): Kaneko K, Nyuji M, Shiraishi T, Kitano H, Shimizu A, Yamaguchi A, Matsuyama M
  • Organizer: 5^<th> World Fisheries Congress, 3a12
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 2008-10-22
• [Remarks] ホームページ等
  • URL: http://bbs1.agr.kyushu-u.ac.jp/asweb/suil/lmb.html