2010 Fiscal Year Final Research Report
Molecular mechanism of the function of fish egg lectins and its application
| Project/Area Number |
20380118
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Fisheries chemistry
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| Research Institution | Tohoku University |
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Principal Investigator |
MURAMOTO Koji Tohoku University, 大学院・生命科学研究科, 教授 (90157800)
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| Co-Investigator(Kenkyū-buntansha) |
OGAWA Tomohisa 東北大学, 大学院・生命科学研究科, 准教授 (80240901)
NAGANUMA Takako 東北大学, 大学院・生命科学研究科, 助教 (50250733)
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| Project Period (FY) |
2008 – 2010
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| Keywords | レクチン / 魚類卵 / 糖鎖 / 生体防御 / ラムノース |
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| Research Abstract |
The molecular mechanism of the functions of rhamnose-binding lectins (RBLs) from fish eggs was revealed. RBL played a role in biodefense by binding to a microsporidian as well as bacteria via glycolipids and lipopolysaccharides. The precise structures of three RBLs, CSL1, CSL2 and CSL3, from chum salmon were analyzed by using a novel chromatographic technique and X-ray crystallography. CSLs had the opsonic effect on the macrophage cell line from rainbow trout and induced the production of proinflammatory cytokines and reactive oxygen species. They were applied to reduce the infiltration of an opportunistic bacterium and induce the apoptotic death of human colon adenocarcinoma cell lines. Furthermore, the expression system for recombinant RBLs was constructed.
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[Journal Article] The function of rhamnose-binding lectin in innate immunity by restricted binding to Gb3.2009
Author(s)
Y.Watanabe, H.Tateno, S.Nakamura-Tsuruta, J.Kominami, J.Hirabayashi, O.Nakamura, T.Watanabe, H.Kamiya, T.Naganuma, T.Ogawa, R.J.Naude, K.Muramoto
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Journal Title
Dev.Comp.Immunol. 33
Pages: 187-197
Peer Reviewed
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[Journal Article] Isolation and characterization of L-rhamnose-binding lectin, which binds to microsporidian, Glugea plecoglossi, from ayu (Plecoglossus altivelis) eggs.2008
Author(s)
Y.Watanabe, N.Shiina, F.Shinozaki, H.Yokoyama, J.Kominami, S.Nakamura-Tsuruta, J.Hirabayashi, K.Sugahara, H.Kamiya, H.Matsubara, T.Ogawa, K.Muramoto
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Journal Title
Dev.Comp.Immunol. 32
Pages: 487-499
Peer Reviewed
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