2010 Fiscal Year Final Research Report
Cryopreservation of oocytes and embryos by inducing the expression of cryoprotectant channels.
| Project/Area Number |
20380155
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied animal science
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| Research Institution | Kochi University |
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Principal Investigator |
EDASHIGE Keisuke Kochi University, 教育研究部・総合科学系, 教授 (30175228)
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| Co-Investigator(Kenkyū-buntansha) |
KASAI Magosaburo 高知大学, 教育研究部・総合科学系, 教授 (60152617)
KOSHIMOTO Chihiro 宮崎大学, フロンティア科学実験総合センター, 教授 (70295210)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 凍結保存 / 卵子 / 胚 / 水透過性 / 耐凍剤透過性 / アクアポリン |
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| Research Abstract |
We tried to develop a vitrification method by which mouse oocytes and embryos at various stages can be cryopreserved by a single method. First, we examined whether the membrane-permeability of mouse oocytes and embryos can be equalized, because the permeability largely affects the condition for vitrification. We tried to induce the expression of water/cryoprotectant channels (AQP) in oocytes to increase the low membrane-permeability. We also tried in morulae to decrease the permeability of AQP3 which is markedly expressed in morulae and blastocysts. The cryoprotectant-permeability of oocytes increased slightly by retinoic acid but the maturation rate decreased remarkably. It was reported that the permeability of AQP3 decreased markedly at low pH. However, the permeability to ethylene glycol of mouse morulae, which expressed AQP3 abundantly, increased at low pH. Therefore, we considered that it would be difficult to make the membrane-permeability of oocytes/embryos equal. Next, we tried to develop a new vitrification solution in which a high concentration of sucrose was included to reduce the effect of membrane-permeability of oocytes/embryos on the vitrification condition. With this solution, embryos from the 2-cell to morula stages were successfully vitrified in the same condition, and vitrified embryos could be transported with dry ice (at -79℃). The method would be useful for cryopreservation of mouse embryos at various stages.
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Research Products
(38 results)
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[Journal Article] Human C-reactive protein enhances thrombus formation after neointimal balloon injury in transgenic rabbits.2011
Author(s)
S.Matsuda, A.Yamashita, Y.Sato, S.Kitajima, T.Koike, C.Sugita, S.Moriguchi-Goto, K.Hatakeyama, M.Takahashi, C.Koshimoto, Y. Matsuura, T.Iwakiri, Y.E. Chen, J.Fan, Y.Asada.
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Journal Title
The Journal of Thrombosis and Haemostasis 9
Pages: 201-208
Peer Reviewed
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[Journal Article] Equilibrium vitrification of mouse embryos.2010
Author(s)
Bo Jin, Keiji Mochida, Atsuo Ogura, Eri Hotta, Yukiko Kobayashi, Kaori Ito, Go Egawa, Shinsuke Seki, Hiroshi Honda, Keisuke Edashige, Magosaburo Kasai.
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Journal Title
Biology of Reproduction 82
Pages: 444-450
Peer Reviewed
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[Journal Article] Analysis of antibody response by temperature-sensitive measles vaccine strain in the cotton rat model.2009
Author(s)
Takeshi Haga, Niho Murayama, Yuya Shimizu, Akatsuki Saito, Takumi Sakamoto, Tetsuo Morita, Katsuhiro Komase, Tetsuo Nakayama, Kazuyuki Uchida, Tetsuro Katayama, Akio Shinohara, Chihiro Koshimoto, Hiroshi Sato, Hironori Miyata, Kiyoaki Katahira, Yoshitaka Goto.
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Journal Title
Comparative Immunology, Microbiology and Infectious Diseases 32
Pages: 395-406
Peer Reviewed
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[Presentation] Equilibrium vitrification of mouse embryos.2009
Author(s)
Bo Jin, Eri Hotta, Yukiko Kobayashi, Kaori Ito, Go Egawa, Shinsuke Seki, Hiroshi Honda, Keiji Mochida, Atsuo Ogura, Keisuke Edashige, Magosaburo Kasai
Organizer
46th Annual Meeting of the Society for Cryobiology
Place of Presentation
Sapporo, Conference Hall on Hokkaido University.
Year and Date
20090719-20090723
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[Presentation] 様々な発育ステージのガラス化凍結マウス胚の-80℃での短期保持-ドライアイスを用いた簡便な輸送を目指して-2009
Author(s)
金波, 堀田英里, 小林由希子, 伊藤香, 関信輔, 本田宙, 持田慶司, 小倉淳郎, 葛西孫三郎, 枝重圭祐
Organizer
第56回日本実験動物学会総会
Place of Presentation
大宮,大宮ソニックシティ
Year and Date
20090514-20090516
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[Presentation] Short-term storage of vitrified two-cell mouse embryos at -80oC for handy transportation with dry ice.2008
Author(s)
Bo Jin, Eri Hotta, Yukiko Kobayashi, Shinsuke Seki, Masahiro Yoshimura, Hiroshi Honda, Keiji Mochida, Atsuo Ogura, Magosaburo Kasai, Keisuke Edashige
Organizer
第101回日本繁殖生物学会大会
Place of Presentation
福岡市,九州大学医学部百年講堂
Year and Date
20080918-20080920
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[Presentation] ウシ桑実胚の水透過と耐凍剤透過におけるaquaporin 3の役割2008
Author(s)
河合泰典, 原隆夫, 松尾完, Valdez M.Delgado Jr., 金波, 好村正博, 東山龍一, 市川恭子, 恒石望太郎, 枝重圭祐, 葛西孫三郎
Organizer
第101回日本繁殖生物学会大会
Place of Presentation
福岡市,九州大学医学部百年講堂
Year and Date
20080918-20080920
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