2012 Fiscal Year Final Research Report
Manipulation of chicken primordial germ cells and its application to producing transgenic chickens
| Project/Area Number |
20380156
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied animal science
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| Research Institution | National Institute of Agrobiological Sciences |
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Principal Investigator |
NAITO Mitsuru 独立行政法人農業生物資源研究所, 動物発生分化研究ユニット, 上級研究員 (70355733)
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| Project Period (FY) |
2008 – 2012
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| Keywords | 始原生殖細胞 / 外来遺伝子 / トランスフェクション / GFP遺伝 / ニワトリ初期胚 / 生殖系列キメラ / 品種識別 / 体外培養 |
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| Research Abstract |
In order to develop a technique producing transgenic chickens, we attempted to culture primordial germ cells isolated from early-stage embryos in vitro for long-term. The cultured primordial germ cells retained the ability to migrate to gonads after transfer into recipient embryos and also confirmed the ability of differentiating to functional gametes in recipient gonads. Introduction of GFP gene into the cultured primordial germ cells was performed and cultured primordial germ cells expressing GFP gene were successfully entered gonads of recipient embryos.
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