In silico analysis of full-length rich cDNA library of Echinococcus multilocularis, molecular bases of the parasite growth and development, and vaccine

2010 Fiscal Year Final Research Report

• Project/Area Number: 20380164
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied veterinary science
• Research Institution: Hokkaido University
• Principal Investigator: OKU Yuzaburo Hokkaido University, 農学部, 教授 (60133716)
• Co-Investigator(Kenkyū-buntansha): NONAKA Nariaki 宮崎大学, 農学部, 准教授 (50281853) ; WATANABE Junichi 東京大学, 医科学研究所, 助教 (20201189) ; SUGIMOTO Chihiro 北海道大学, 人獣共通感染症リサーチセンター, 教授 (90231373) ; MATSUMOTO Jun 日本大学, 生物資源科学部, 講師 (70296169) ; YAGIU Kinpei 北海道立衛生研究所, 生物科学部, 衛生動物科長 (70414323)
• Project Period (FY): 2008 – 2010
• Keywords: エキノコックス / 網羅的cDNAライブラリー / RNA干渉 / 発育段階 / テトラスパニン / 組み換えワクチン / 糖蛋白 / in silico解析

Research Abstract

Echinococcus multilocularis causes a severe disease in humans. We produced full-length rich cDNA libraries of larval and adult E. multilocularis. About 9,500 clones and 12,100 clones (excluding host DNA) were curried out 5' one-pass sequencing for the adult and the larva, respectively, and in silico analysis of the data were curried out. Several candidate molecules were examined in experiments, in the consequences, potency of tetraspanins and ycoproteins were suggested. RNA inference is a important method for the biological function analysis. We stablished this method in cestode parasite.

Research Products

• [Journal Article] Echinococcus multilocularis : purification and characterization of glycoprotein antigens with serodiagnostic potential for canine infection. (2011)
  • Author(s): Kouguchi H, Matsumoto J, Yamano K, Katoh Y, Oku Y, Suzuki T, Yagi K
  • Journal Title: Exp Parasitol. 128(1) Pages: 50-56
  • Peer Reviewed
• [Journal Article] Development of PCR/dot blot assay for specific detection and differentiation of taeniid cestode eggs in canids. (2011)
  • Author(s): Armua-Fernandez MT, Nonaka N, Sakurai T, Nakamura S, Gottstein B, Deplazes P, Phiri IG, Katakura K, Oku Y.
  • Journal Title: Parasitol Int. 60(1) Pages: 84-89
  • Peer Reviewed
• [Journal Article] 多包条虫(幼虫・成虫)のcDNAライブラリのin silico解析特にspliced leaderを有する遺伝子について (2010)
  • Author(s): 奥祐三郎、八木欣平、原雄一郎、渡辺日出海、李爽、山下理宇、若栗浩幸、鈴木穣、豊田敦、渡辺純一
  • Journal Title: 獣医寄生虫学会 8巻 Pages: 116
• [Journal Article] 種特異的オリゴプローブを用いた犬のテニア科条虫の同定と鑑別 (2010)
  • Author(s): Armua-Fernandez MT, Nonaka N, Sakurai T, Nakamura S, Gottstein B, Deplazes P, Phiri IG, Katakura K, Oku Y.
  • Journal Title: 獣医寄生虫学会 8巻 Pages: 117
• [Journal Article] Gene silencing in Echinococcus multilocularis protoscoleces using RNA interference. (2010)
  • Author(s): Mizukami C, Spiliotis M, Gottstein B, Yagi K, Katakura K, Oku Y.
  • Journal Title: Parasitol Int. 59(4) Pages: 647-652
  • Peer Reviewed
• [Journal Article] Echinococcus multilocularis primary cells : improved isolation, small-scale cultivation and RNA interference. (2010)
  • Author(s): Spiliotis M, Mizukami C, Oku Y, Kiss F, Brehm K, Gottstein B.
  • Journal Title: Mol Biochem Parasitol. 174(1) Pages: 83-87
  • Peer Reviewed
• [Journal Article] Primary alveolar echinococcosis : course of larval development and antibody responses in intermediate host rodents with different genetic backgrounds after oral infection with eggs of Echinococcus multilocularis. (2010)
  • Author(s): Matsumoto J, Kouguchi H, Oku Y, Yagi K
  • Journal Title: Parasitol Int. 59(3) Pages: 435-444
  • Peer Reviewed
• [Journal Article] Echinococcus multilocularis : two-dimensional Western blotting method for the identification and expression analysis of immunogenic proteins in infected dogs. (2010)
  • Author(s): Kouguchi H, Matsumoto J, Katoh Y, Suzuki T, Oku Y, Yagi K
  • Journal Title: Exp Parasitol. 124(2) Pages: 238-243
  • Peer Reviewed
• [Journal Article] An Echinococcus multilocularis coproantigen is a surface glycoprotein with unique O-gycosylation. (2010)
  • Author(s): Hulsmeier AJ, Deplazes P, Naem S, Nonaka N, Hennet T, Kohler P.
  • Journal Title: Glycobiology. 20(1) Pages: 127-135
  • Peer Reviewed
• [Journal Article] Evaluation of Echinococcus multilocularis tetraspanins as vaccine candidates against primary alveolar echinococcosis. (2009)
  • Author(s): Dang Z, Yagi K, Oku Y, Kouguchi H, Kajino K, Watanabe J, Matsumoto J, Nakao R, Wakaguri H, Toyoda A, Sugimoto C.
  • Journal Title: Vaccine. 27(52) Pages: 7339-7345
  • Peer Reviewed
• [Journal Article] Multiplex PCR system for identifying the carnivore origins of faeces for an epidemiological study on Echinococcus multilocularis in Hokkaido, Japan. (2009)
  • Author(s): Nonaka N, Sano T, Inoue T, Teresa Armua M, Fukui D, Katakura K, Oku Y.
  • Journal Title: Parasitol Res. 106(1) Pages: 75-83
  • Peer Reviewed
• [Journal Article] Molecular cloning and characterization of a T24-like protein in Echinococcus multilocularis. (2009)
  • Author(s): Dang Z, Watanabe J, Kajino K, Oku Y, Matsumoto J, Yagi K, Kouguchi H, Sugimoto C.
  • Journal Title: Mol Biochem Parasitol. 168(1) Pages: 117-119
  • Peer Reviewed
• [Journal Article] Monitoring of environmental contamination by Echinococcus multilocularis in an urban fringe forest park in Hokkaido, Japan. (2009)
  • Author(s): Lagapa JT, Oku Y, Kaneko M, Ganzorig S, Ono T, Nonaka N, Kobayashi F, Kamiya M.
  • Journal Title: Environ Health Prev Med. 14(5) Pages: 299-303
  • Peer Reviewed
• [Journal Article] Echinococcus multilocularis infection in pet dogs in Japan. (2009)
  • Author(s): Nonaka N, Kamiya M, Kobayashi F, Ganzorig S, Ando S, Yagi K, Iwaki T, Inoue T, Oku Y.
  • Journal Title: Vector Borne Zoonotic Dis. 9(2) Pages: 201-206
  • Peer Reviewed
• [Journal Article] A vague understanding of the biology and epidemiology of echinococcosis by dog owners in Hokkaido, an endemic island for Echinococcus multilocularis in Japan. (2009)
  • Author(s): Nonaka N, Kamiya M, Oku Y.
  • Journal Title: J Vet Med Sci. 71(1) Pages: 105-107
  • Peer Reviewed
• [Journal Article] The first instance of a cat excreting Echinococcus multilocularis eggs in Japan. (2008)
  • Author(s): Nonaka N, Hirokawa H, Inoue T, Nakao R, Ganzorig S, Kobayashi F, Inagaki M, Egoshi K, Kamiya M, Oku Y.
  • Journal Title: Parasitol Int. 57(4) Pages: 519-520
  • Peer Reviewed
• [Presentation] マウスにおける多包条虫シスト定着と原頭節発育に関与する宿主側遺伝的因子のQTL解析 (2011)
  • Author(s): 中尾亮、亀田弥生、孝口裕一、Yila Simon、松本淳、鳥越大輔、佐々木宣哉、奥祐三郎、杉本千尋、安居院高志、八木欣平
  • Organizer: 第151回日本獣医学会学術集会
  • Place of Presentation: 東京農工大学
  • Year and Date: 2011-03-31
• [Presentation] 多包虫感染コットンラットの腹腔内シスト塊由来cDNA ライブラリーに含まれていた宿主由来cDNAの解析 (2010)
  • Author(s): 奥祐三郎、八木欣平、原雄一郎、渡辺日出海、李爽、山下理宇、若栗浩幸、鈴木穣、豊田敦、渡辺純一
  • Organizer: 第150回日本獣医学会学術集会
  • Place of Presentation: 帯広畜産大学
  • Year and Date: 2010-09-17
• [Presentation] In silico analyses of full-length rich cDNA libraries of larval and adult Echinococcus multilocularis (Preliminary results). (2009)
  • Author(s): Oku Y., Armua-Fernandez M.T., Yagi Y, et al
  • Organizer: Workshop on "Recent advances in the biology and biochemistry of Echinococcus infections", in the context of the XXIII World Congresss of Hydatidosis.
  • Place of Presentation: Hotel Raisson, Colonia del Sacramento, Uruguay
  • Year and Date: 2009-12-10
• [Presentation] RNA interference (RNAi) methods for protoscoleces and primary cells of Echinococcus multilocularis. (2009)
  • Author(s): Mizukami, C., Spiliotis M., Gottstein, B., Katakura, K., Oku, Y
  • Organizer: The XXIII World Congresss of Hydatidosis.
  • Place of Presentation: Hotel Raisson, Colonia del Sacramento, Uruguay
  • Year and Date: 2009-12-10
• [Presentation] DNA dot blot assay for the differentiation of Taeniid cestodes in canids. (2009)
  • Author(s): Armua-Fernandez, M.T., Nonaka, N., Sakurai, T., Gosttstein, B., Deplazes, P., Katakura, K., Oku, Y
  • Organizer: The XXIII World Congresss of Hydatidosis.
  • Place of Presentation: Hotel Raisson, Colonia del Sacramento, Uruguay
  • Year and Date: 2009-12-10
• [Presentation] 幼虫および成虫の多包条虫cDNAライブラリーに多く見られたcDNAのin silico解析、特にAntigenBとLSU rRNAについて (2009)
  • Author(s): 奥祐三郎、八木欣平、原雄一郎、渡辺日出海、李爽、山下理宇、若栗浩幸、鈴木穣、豊田敦、渡辺純一
  • Organizer: 第55回日本寄生虫学会・日本衛生動物学会
  • Place of Presentation: 帯広市 帯広畜産大学
  • Year and Date: 2009-10-03
• [Presentation] Discrimination of taeniid cestodes in canids by using species -specific oligonucleotide probes (2009)
  • Author(s): Armua-Fernandez, M.T., Nonaka, N., Sakurai, T., Katakura,K., Oku,Y.
  • Organizer: 第55回日本寄生虫学会・日本衛生動物学会
  • Place of Presentation: 帯広市 帯広畜産大学
  • Year and Date: 2009-10-03
• [Presentation] 多包条虫(幼虫・成虫)のcDNAライブラリーのin silico解析 特にSpliced Leaderを有する遺伝子について (2009)
  • Author(s): 奥祐三郎、八木欣平、原雄一郎、渡辺日出海、李爽、山下理宇、若栗浩幸、鈴木穣、豊田敦、渡辺純一
  • Organizer: 第148回日本獣医学会学術集会
  • Place of Presentation: 鳥取市 鳥取市民会館
  • Year and Date: 2009-09-26
• [Presentation] 人と動物の共通感染症:エキノコックス症の問題解決に向けて (2009)
  • Author(s): 奥祐三郎
  • Organizer: 第32回日本骨・関節感染症学会(特別講演)招待講演
  • Place of Presentation: 札幌市京王プラザホテル(シーガイヤ)
  • Year and Date: 2009-06-19
• [Presentation] 多包虫のcDNAライブラリに見られた抗酸化活性関連遺伝子 (2009)
  • Author(s): 平松澪、水上智秋、松本淳、野中成晃、片倉賢、李爽、山下理宇、渡辺純一、神谷正男、奥祐三郎
  • Organizer: 第78回日本寄生虫学会大会
  • Place of Presentation: 東京 法政大学市ヶ谷キャンパス
  • Year and Date: 2009-03-28
• [Presentation] 遺伝子工学的手法を用いた多包虫に対する組換えモノクローナル抗体の作成 (2009)
  • Author(s): 後藤明子、八木欣平、山野公明
  • Organizer: 第78回日本寄生虫学会大会
  • Place of Presentation: 東京 法政大学市ヶ谷キャンパス
  • Year and Date: 2009-03-28
• [Presentation] Control of echinococcus and molecular biology (2008)
  • Author(s): 奥祐三郎
  • Organizer: Parasite Vector Genomics II in Sapporo
  • Place of Presentation: Sapporo, Hokkaido Univ.
  • Year and Date: 2008-10-22
• [Presentation] 多包条虫の網羅的cDNAライブラリのin silico解析、特にプロテアーゼ類について (2008)
  • Author(s): 奥祐三郎、李爽、山下理宇、渡辺純一、野中成晃、神谷正男
  • Organizer: 第146回日本獣医学会学術集会
  • Place of Presentation: 宮崎市 ワールドコンベンションセンターサミット(シーガイヤ)
  • Year and Date: 2008-09-25
• [Presentation] ザンビアの牛から検出された単包条虫のgenotypeについて (2008)
  • Author(s): 中村星太、野中成晃、I.G.KPhiri、C.Mwelwa、井上貴史、松本淳、片倉賢、奥祐三郎
  • Organizer: 第146回日本獣医学会学術集会
  • Place of Presentation: 宮崎市 ワールドコンベンションセンターサミット
  • Year and Date: 2008-09-25
• [Remarks] ホームページ等
• [Remarks] フルエキノコック
  • URL: http://fullmal.hgc.jp/em/