Functional analysis of equine herpesvirus-1 receptor and its application for infection prevention

2011 Fiscal Year Final Research Report

• Project/Area Number: 20380165
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied veterinary science
• Research Institution: Hokkaido University
• Principal Investigator: KIMURA Takashi 北海道大学, 人獣共通感染症リサーチセンター, 准教授 (90261338)
• Co-Investigator(Kenkyū-buntansha): SAWA Hirofumi 北海道大学, 人獣共通感染症リサーチセンター, 教授 (30292006)
• Project Period (FY): 2008 – 2011
• Keywords: ヘルペスウイルス / ウマ / レセプター

Research Abstract

We analyzed the function of equine MHC class I molecule A68, which was cloned from an equine brain microvascular endothelial cell cDNA expression library as an EHV-1 receptor. Studies suggest that equine MHC class I is a functional gD receptor that plays a pivotal role in EHV-1 entry into equine cells, and also suggest that the amino acid residue at position 173 of the MHC class I molecule is involved in the efficiency of EHV-1 entry. In addition, we generated transgenic mice expressing A68, and also evaluated the usefulness of soluble forms of A68 molecules for preventing EHV-1 infection.

Research Products

• [Journal Article] A single amino acid residue in the α2 domain of major histocompatibility complex class I is involved in the efficiency of equine herpesvirus-1 entry (2011)
  • Author(s): Sasaki, M., Kim, E., Igarashi, M., Ito, K., Hasebe, R., Fukushi, H., Sawa, H., Kimura, T.
  • Journal Title: J. Biol. Chem. Volume: 286 Pages: 39370-39378
  • Peer Reviewed
• [Journal Article] Equine major histocompatibility complex class I molecules act as entry receptors that bind to equine herpesvirus-1 glycoprotein D. (2011)
  • Author(s): Sasaki, M., Hasebe, R., Makino, Y., Suzuki, T., Fukushi, H., Okamoto, M., Matsuda, K., Taniyama, H., Sawa, H. and Kimura, T.
  • Journal Title: Genes Cells Volume: 16 Pages: 343-357
  • Peer Reviewed
• [Journal Article] The human polyoma JC virus agnoprotein acts as a viroporin (2010)
  • Author(s): Suzuki, T., Orba, Y., Okada, Y., Sunden, Y., Kimura, T., Tanaka, S., Nagashima, K., Hall, W. W. and Sawa, H.
  • Journal Title: PLos Pathogens Volume: 6(3) Pages: e1000801
  • Peer Reviewed
• [Journal Article] Large T antigen promotes JC virus replication in G2-arrested cells by inducing ATM-and ATR-mediated G2 checkpoint signaling (2010)
  • Author(s): Orba, Y., Suzuki, T., Makino, Y., Kubota, K., Tanaka, S., Kimura, T., Sawa, H.
  • Journal Title: J. Biol. Chem. Volume: 285 Pages: 1544-54
  • Peer Reviewed
• [Journal Article] Infectious entry of equine herpesvirus-1 into host cells through different endocytic pathways (2009)
  • Author(s): Hasebe, R., Sasaki, M., Sawa, H., Wada, R., Umemura, T., Kimura, T.
  • Journal Title: Virology Volume: 393 Pages: 198-209
  • Peer Reviewed
• [Journal Article] Pharmacological cdk inhibitor R-Roscovitine suppresses JC virus proliferation (2008)
  • Author(s): Orba, Y., Sunden, Y., Suzuki, T., Nagashima, K., Kimura, T., Tanaka, S., Sawa, H.
  • Journal Title: Virology Volume: 370 Pages: 173-83
  • Peer Reviewed
• [Presentation] ウマMHCクラスIが示すウマヘルペスウイルス1型レセプター機能の解析 (2011)
  • Author(s): 佐々木道仁、五十嵐学、澤洋文、伊藤公人、福士秀人、木村享史
  • Organizer: 第152回日本獣医学会学術集会
  • Place of Presentation: 大阪府立大学(堺)
  • Year and Date: 2011-09-20
• [Presentation] ウマMHCクラスI遺伝子導入マウスのウマヘルペスウイルス1型に対する感受性 (2011)
  • Author(s): 木村享史、佐々木道仁、金亨振、福士秀人、澤洋文
  • Organizer: 第152回日本獣医学会学術集会
  • Place of Presentation: 大阪府立大学(堺)
  • Year and Date: 2011-09-19
• [Presentation] Single amino acid residue in equine major histocompatibility complex class I is critical for its function as an equine herpesvirus-1 receptor (2011)
  • Author(s): Sasaki, M., Igarashi, M., Sawa, H., Hasebe, R., Fukushi, H., Kimura, T.
  • Organizer: XV International Congress of Virology (IUMS 2011 Congress)
  • Place of Presentation: Sapporo Convention Center (Sapporo)
  • Year and Date: 2011-09-13
• [Presentation] Novel entry pathway of equine herpesvirus-1 (2010)
  • Author(s): 佐々木道仁,長谷部理絵,澤洋文,福士秀人,谷山弘行,木村享史
  • Organizer: 第33回日本分子生物学会年会.
  • Place of Presentation: 神戸ポートアイランド(神戸)
  • Year and Date: 2010-12-07
• [Presentation] 新規エントリーレセプターを介したウマヘルペスウイルス1型の細胞内侵入機構の解析 (2010)
  • Author(s): 佐々木道仁,長谷部理絵,福士秀人,谷山弘行,澤洋文,木村享史
  • Organizer: 第150回日本獣医学会学術集会
  • Place of Presentation: 帯広畜産大学(帯広)
  • Year and Date: 2010-09-16
• [Presentation] ウマヘルペスウイルス1型レセプターのクローニングと機能解析 (2009)
  • Author(s): 佐々木道仁,長谷部理絵,谷山弘行,澤洋文,木村享史
  • Organizer: 第57回日本ウイルス学会学術集会
  • Place of Presentation: 都市センターホテル(東京)
  • Year and Date: 2009-10-25
• [Book] In Maeda A. ed. Animal Viruses (2010)
  • Author(s): Kimura, T.
  • Total Pages: 133-141
  • Publisher: Pathogenesis of equine herpesvirus-1 infection