Comprehensive study of fungal biosurfactant proteins that recruit polymer-degrading enzymes onto solid polymer-surfaces

2010 Fiscal Year Final Research Report

• Project/Area Number: 20380175
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Boundary agriculture
• Research Institution: Tohoku University
• Principal Investigator: ABE Keietsu 東北大学, 大学院・農学研究科, 教授 (50312624)
• Co-Investigator(Kenkyū-buntansha): SANO Motoaki 金沢工業大学, ゲノム生物工学研究所, 講師 (80410299)
• Project Period (FY): 2008 – 2010
• Keywords: バイオマス / 固体醗酵 / 界面活性蛋白質 / hydrophobin

Research Abstract

hen the industrial fungus Aspergillus oryzae was grown on cellulose and keratin as solid polymer substrates, two small bio-surfactant proteins (Mw <20 kDa)that were attached to the solid surfaces were isolated. Two genes encoding the two proteins were cloned and highly expressed in A. oryzae. The two purified bio-surfactant proteins attached to the surface of cellulose recruited xylanase F3 (XynF3)which lacks a carbohydrate biding domain. In vitro, molecular interaction analysis revealed the two proteins immobilized on the solid surfaces possessed dissociation constants KD. 10μM order to XynF3. We also analyzed interaction of esterase with another surfactant protein RolA attached to the two solid materials

Research Products

• [Journal Article] Characterization of the conserved phosphorylation site in the Aspergillus nidulans response regulator Sera (2011)
  • Author(s): Hagiwara D., T. Mizuno, and K. Abe
  • Journal Title: Curr. Genet Volume: 57 Pages: 103-114
  • Peer Reviewed
• [Journal Article] 麹菌固体発酵システムによる新規バイオプロセスへの挑戦-生分解性プラスチックのバイオケミカルリサイクル- (2009)
  • Author(s): 阿部敬悦, 上原健二, 高橋徹, 大滝真作, 前田浩, 山形洋平, 五味勝也, 長谷川史彦
  • Journal Title: 日本醸造協会誌 Volume: 104 Pages: 10-18
• [Journal Article] Genes regulated by AoXlnR, the xylanolytic and cellulolytic transcriptional regulator (2009)
  • Author(s): Noguchi Y, Sano M, Kanamaru K, Ko T, Takeuchi M, Kato M, Kobayashi T
  • Journal Title: Appl Microbiol Biotechnol Volume: 85 Pages: 141-154
  • Peer Reviewed
• [Presentation] Glu31, Asp142 and Asp171 of Aspergillus oryzae cutinase CutL1 are required for both interaction with hydrophobin Rolla and consequent stimulation of polyester-degradation (2011)
  • Author(s): Muragaki K., et al
  • Organizer: 26th Fungal Genetics Conference
  • Place of Presentation: Asilomar米国
  • Year and Date: 20110315-20
• [Presentation] Novel functions of fungal biosurfactant proteins in degradation of biopolymers : Aspergillus oryzae hydrophobin Rolla laterally moves on hydrophobic surfaces and recruits polyesterases. (2009)
  • Author(s): Abe K., et al
  • Organizer: Korean Fungal Genetics and Biology Conference
  • Place of Presentation: Seoul National University, Seoul Korea
  • Year and Date: 20090114-15
• [Presentation] セルロースに吸着しているタンパク質の決定 (2009)
  • Author(s): 佐野元昭、北川治恵、阿部敬悦, 大箸信一
  • Organizer: セルロース学会
  • Place of Presentation: 北海道大学札幌
  • Year and Date: 2009-07-03
• [Book] Novel Industrial Applications of Aspergillus oryzae Genomics. Aspergillus : Molecular Biology and Genomics, Chapter 10 (2010)
  • Author(s): Abe K., K. Furukawa, T. Fujioka, D. Hagiwara, H. Maeda, J. Marui, O. Mizutani, T. Takahashi, A. Yoshimi, Y. Yamagata, K. Gomi, F. Hasegawa
  • Total Pages: 199-227
  • Publisher: Caister Academic Press
• [Remarks]
  • URL: http://www.niche.tohoku.ac.jp/index.php?page=project_abe