2012 Fiscal Year Final Research Report
Study on the glycinebetaine synthesis, transport and accumulation in the Graminea
| Project/Area Number |
20380177
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Boundary agriculture
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| Research Institution | Meijo University (2010-2012)
Nagoya University (2008-2009) |
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Principal Investigator |
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| Project Period (FY) |
2008 – 2012
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| Keywords | 環境 / 植物 / ストレス / 発現制御 / バイオテクノロジー |
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| Research Abstract |
Osmoprotectant glycinebetaine synthesis enzymes from choline to glycine betaine was known only in Chenopodiaceae (spinach, sugar beet and halophytes) , although glycinebetaine is synthesized and accumulated in several plant families. We tried to find out the glycinebetaine synthesis enzymes in Gramineae, one of the most important crop plants using barley as experimental plant. We succeeded to characterize barley novel colinemonooxygenase (HvCMO) and betaine aldehyde dehydrogenases (HvBADH1 and HvBADH2) and determined the intercellular localization of HvCMO (peroxisome), HvBADH1 (peroxisome) andHvBADH2 (cytosol). These findings are totally unique and different from those of the Chenopodiaceae glycinebetaine synthetic enzymes in chloroplasts. We also studied on a novel glycinebetaine/proline transporter gene expressed in the mestome sheath and lateral root cap cells in barley. This finding suggests that plants have complicated but impressive glycinebetaine long distance transport systems.
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Research Products
(16 results)
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[Journal Article] Isolation and characterization of a novel peroxisomal cholinemonooxydase in barley.2011
Author(s)
Mitsuya, S., Kuwabara, J., Ozaki,K., Saeki,E., Fujiwara, T. and Takabe,Tetsuko
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Journal Title
Planta
Volume: 234
Pages: 1215-1226
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[Journal Article] Enrichment of sugar content in melon fruits by hydrogen peroxide treatment. J.2009
Author(s)
Ozaki, K., Uchida, A., Takabe, Tomoko, Shinagawa, F., Tanaka, Y., Takabe, Teruhiro, Hayashi, T., Hattori, A., Rai, A. K. and Takabe,Tetsuko
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Journal Title
Plant Physiol.
Volume: 166
Pages: 569-578
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[Journal Article] Enzymatic characterization of peroxisomal and cytosolic betaine aldehyde dehydrogenases in barley.2008
Author(s)
Fujiwara, T., Hori, K., Ozaki, K., Yokota, Y., Mitsuya, S., Ichiyanagi, T., Mori, N., Hattori, T. and Takabe, Tetsuko
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Journal Title
Physiol. Plant
Volume: 134
Pages: 22-28
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[Journal Article] Overexpression of DnaK chaperon from a halotolerant cyanobacterium Aphanothece halophytica increases seed yield in rice and tobacco.2008
Author(s)
Uchida, A., Hibino, T., Shimada, T, Saigusa, M., Takabe, Teruhiro, Araki, E., Kajita, H. and Takabe, Tetsuko
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Journal Title
Plant Biotechnology
Volume: 25
Pages: 141-150
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