2010 Fiscal Year Final Research Report
Proteomics of bile acid signals
| Project/Area Number |
20390009
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Physical pharmacy
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| Research Institution | Tohoku University |
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Principal Investigator |
GOTO Junichi Tohoku University, 病院, 名誉教授 (80006337)
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| Co-Investigator(Kenkyū-buntansha) |
MANO Nariyasu 東北大学, 病院, 教授 (50323035)
SHIMADA Miki 東北大学, 病院, 准教授 (10196488)
YAMAGUCHI Hiroaki 東北大学, 病院, 助教 (80400373)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 分析化学 / 機能性器材 / 脳 / トランスポーター / 胆汁酸 / プロテオーム |
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| Research Abstract |
According to proteomics approach, we tried to develop an analytical method for bile acid signal transduction and clarify the function of bile acids in the brain. The results by the affinity labeling analysis of growth hormone using chenodeoxycholyl adenylate showed that chenodeoxycholate binds to the region different from the growth hormone receptor binding site. Next, we developed an analytical method of phosphorylated proteins using the specific derivatization and the double pseudo-neutral loss extraction on nanoLC/ESI-MS/MS. We found its usefulness for identification of phosphorylated proteins in complex protein mixtures and identification of phosphorylated sites. In addition, we extracted chenodeoxycholate-binding proteins in hepatocytes by using the chenodeoxycholate-immobilized cleavable affinity gel, and identified dihydrodiol dehydrogenase, which is known as a bile acid-binding protein in the hepatocyte, and peroxiredoxin 1.
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[Presentation] Determination of multi-conjugated bile acids in the urine of patients with Niemann-Pick disease, type C by liquid chromatography/electrospray ionization tandem mass spectrometry.2010
Author(s)
Masamitsu Maekawa, Yasushi Misawa, Ayako Sotoura, Osamu Sakamoto, Toshihiro Ohura, Daiki Abukawa, Takehiko Inoue, Kousaku Ohno, Akina Muto, Hajime Takei, Hiroshi Nittono, Shoujiro Ogawa, Genta Kakiyama, Takashi Iida, Alan F. Hofmann, Junichi Goto, Miki Shimada, Nariyasu Mano
Organizer
XXI International Bile Acid Meeting ; Bile Acids as Metabolic Integrators and Therapeutics Freiburg
Place of Presentation
ドイツ
Year and Date
2010-10-07
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