2010 Fiscal Year Final Research Report
Development of a noninvasive transdermal gene delivery system for gene therapy of diabetes
| Project/Area Number |
20390016
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Physical pharmacy
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| Research Institution | Kyoto Pharmaceutical University |
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Principal Investigator |
KOGURE Kentaro Kyoto Pharmaceutical University, 薬学部, 教授 (70262540)
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| Co-Investigator(Kenkyū-buntansha) |
OKUNO Takashi 富山大学, 薬学部, 准教授 (80411031)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 遺伝子送達 / 糖尿病 / インスリン / 皮膚 |
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| Research Abstract |
In this study, we attempted to develop gene therapeutic technology for diabetes by production of insulin in skin and muscle via noninvasive transdermal delivery of artificial gene delivery system MEND. We succeeded the noninvasive transdermal delivery of MEND by jet-injection, and induction of sustained gene expression in the skin. We constructed preproinsulin plasmid DNA, and confirmed significant expression of the mRNA in the culture cells and skin. Moreover, increment of insulin production in diabetes-model mice treated with MEND.
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[Journal Article] Nanoparticles for ex vivo siRNA delivery to dendritic cells for cancer vaccines : Programmed endosomal escape and dissociation.2010
Author(s)
Akita H, Kogure K, Moriguchi R, Nakamura Y, Higashi T, Nakamura T, Serada S, Fujimoto M, Naka T, Futaki S, Harashima H.
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Journal Title
J.Control.Release 143巻
Pages: 311-317
Peer Reviewed
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[Journal Article] Multi-layered nano particles for penetrating the endosome and nuclear membrane via a step-wise membrane fusion process.2009
Author(s)
Akita H, Kudo A, Minoura A, Yamaguchi M, Khalil IA, Moriguchi R, Masuda T, Danev R, Nagayama K, Kogure K, Harashima H.
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Journal Title
Biomaterials 30巻
Pages: 2940-2949
Peer Reviewed
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