Elucidation of bacterial pathogenesis system based on a silkworm infection model

2010 Fiscal Year Final Research Report

• Project/Area Number: 20390021
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biological pharmacy
• Research Institution: The University of Tokyo
• Principal Investigator: SEKIMIZU Kazuhisa The University of Tokyo, 大学院・薬学系研究科, 教授 (90126095)
• Co-Investigator(Kenkyū-buntansha): KAITO Chikara 東京大学, 大学院・薬学系研究科, 准教授 (60420238)
• Project Period (FY): 2008 – 2010
• Keywords: カイコ / 病原性 / 病原性因子

Research Abstract

In this study, we performed to solve molecular function of CvfB (conserved virulence factor B), which was identified as a virulence regulatory factor using silkworm infection model. CvfB has three S1 RNA binding domain and a Winged-Helix domain revealed by crystal structure at 1.4Å resolution. Moreover, we uncovered the RNA binding activity of CvfB is required for hemolysin production in Staphylococcus aureus.

Research Products

• [Journal Article] Transcription and translation products of the cytolysin gene psm-mec on the mobile genetic element SCCmec regulate Staphylococcus aureus virulence. (2011)
  • Author(s): Kaito C, Saito Y, Nagano G, Ikuo M, Omae Y, Hanada Y, Han X, Kuwahara-Arai K, Hishinuma T, Baba T, Ito T, Hiramatsu K, Sekimizu K.
  • Journal Title: PLoS Pathog. 7(2) Pages: e1001267
  • Peer Reviewed
• [Journal Article] Porphyromonas gingivalis peptidoglycans induce excessive activation of the innate immune system in silkworm larvae. (2010)
  • Author(s): Ishii K, Hamamoto H, Imamura K, Adachi T, Shoji M, Nakayama K, Sekimizu K.
  • Journal Title: J Biol Chem. 285(43) Pages: 33338-33347
  • Peer Reviewed
• [Journal Article] Insect cytokine paralytic peptide (PP) induces cellular and humoral immune responses in the silkworm Bombyx mori. (2010)
  • Author(s): Ishii K, Hamamoto H, Kamimura M, Nakamura Y, Noda H, Imamura K, Mita K, Sekimizu K.
  • Journal Title: J Biol Chem. 285(37) Pages: 28635-28642
  • Peer Reviewed
• [Journal Article] Structure of a virulence regulatory factor CvfB reveals a novel winged helix RNA binding module. (2010)
  • Author(s): Matsumoto Y, Xu Q, Miyazaki S, Kaito C, Farr CL, Axelrod HL, Chiu HJ, Klock HE, Knuth MW, Miller MD, Elsliger MA, Deacon AM, Godzik A, Lesley SA, Sekimizu K, Wilson IA.
  • Journal Title: Structure. 18(4) Pages: 537-547
  • Peer Reviewed
• [Journal Article] The cvfC operon of Staphylococcus aureus contributes to virulence via expression of the thyA gene. (2010)
  • Author(s): Ikuo M, Kaito C, Sekimizu K.
  • Journal Title: Microb Pathog. 49(1-2)(Epub 2010 Mar 27) Pages: 1-7
  • Peer Reviewed
• [Journal Article] Rapid exchange of bound ADP on the Staphylococcus aureus replication initiation protein DnaA. (2009)
  • Author(s): Kurokawa K, Mizumura H, Takaki T, Ishii Y, Ichihashi N, Lee BL, Sekimizu K.
  • Journal Title: J Biol Chem. 284(49) Pages: 34201-34210
  • Peer Reviewed
• [Journal Article] Evaluation of target specificity of antibacterial agents using Staphylococcus aureus ddlA mutants and D-cycloserine in a silkworm infection model. (2009)
  • Author(s): Kurokawa K, Hamamoto H, Matsuo M, Nishida S, Yamane N, Lee BL, Murakami K, Maki H, Sekimizu K.
  • Journal Title: Antimicrob Agents Chemother. 53(9) Pages: 4025-4027
  • Peer Reviewed
• [Journal Article] Purification of a soil bacteria exotoxin using silkworm toxicity to measure specific activity. (2009)
  • Author(s): Usui K, Miyazaki S, Kaito C, Sekimizu K.
  • Journal Title: Microb Pathog. 46(2) Pages: 59-62
  • Peer Reviewed
• [Journal Article] Pleiotropic roles of polyglycerolphosphate synthase of lipoteichoic acid in growth of Staphylococcus aureus cells. (2009)
  • Author(s): Oku Y, Kurokawa K, Matsuo M, Yamada S, Lee BL, Sekimizu K.
  • Journal Title: J Bacteriol. 191(1) Pages: 141-151
  • Peer Reviewed
• [Journal Article] A novel gene, fudoh, in the SCCmec region suppresses the colony spreading ability and virulence of Staphylococcus aureus. (2008)
  • Author(s): Kaito C, Omae Y, Matsumoto Y, Nagata M, Yamaguchi H, Aoto T, Ito T, Hiramatsu K, Sekimizu K.
  • Journal Title: PLoS One. 3(12) Pages: e3921
  • Peer Reviewed
• [Presentation] カイコ幼虫感染症モデルを用いた、バイオアッセイによる感染症治療薬シーズの開発 (2011)
  • Author(s): 関水和久
  • Organizer: 彩都産学官連携シンポジウム
  • Place of Presentation: 大阪
  • Year and Date: 2011-01-27
• [Presentation] 新規病原性遺伝子群を基盤とする病原性発現システムの把握 (2011)
  • Author(s): 関水和久
  • Organizer: 「特定領域」感染現象のマトリックス第7回全体班会議
  • Place of Presentation: 東京
  • Year and Date: 2011-01-13
• [Presentation] 招待講演「Slikworm infection models for evaluation of bacterial pathogenecity and the rapeutic effects of antibiotics」 (2010)
  • Author(s): 関水和久
  • Organizer: 拠点大学交流事業 The 9th NRCT-JSPS Joint Seminar
  • Place of Presentation: バンコク
  • Year and Date: 2010-12-09
• [Presentation] 毒素遺伝子の転写産物と翻訳産物によるメチシリン耐性黄色ブドウ球菌の病原性制御 (2010)
  • Author(s): 垣内力
  • Organizer: 第32回生体膜と薬物の相互作用シンポジウム
  • Place of Presentation: 富山県
  • Year and Date: 2010-11-29
• [Presentation] カイコ創薬のすすめ (2010)
  • Author(s): 関水和久
  • Organizer: 2010年度日本農芸化学会西日本支部若手シンポジウム 招待講演
  • Place of Presentation: 九州大学
  • Year and Date: 2010-10-23
• [Presentation] Use of silkworm for evaluation bacterial pathogenisity and the therapeutic effects of antibiosics (2010)
  • Author(s): 関水和久
  • Organizer: 第10回あわじしま感染症・免疫フォーラム 招待講演
  • Place of Presentation: 兵庫県
  • Year and Date: 2010-09-08
• [Presentation] カイコを利用した医薬品開発 (2010)
  • Author(s): 関水和久
  • Organizer: (社)日本薬剤学会第35回製剤セミナー 招待講演
  • Place of Presentation: 浜松市
  • Year and Date: 2010-07-22
• [Book] 創薬化学の魅力-東京大学大学院薬学系研究科からの発信- (2010)
  • Author(s): 関水和久、垣内力、浜本洋、松本靖彦
  • Total Pages: 263-274
  • Publisher: 廣川書店
• [Remarks] ホームページ
  • URL: http://www.f.u-tokyo.ac.jp/~bisei/