2010 Fiscal Year Final Research Report
Regulation of gene expression involved in conveying information between synapses and nuclei in neurons
| Project/Area Number |
20390023
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | University of Toyama |
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Principal Investigator |
MASAAKI Tsuda University of Toyama, 大学院・医学薬学研究部(薬学), 教授 (80132736)
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| Co-Investigator(Kenkyū-buntansha) |
AKIKO Tabuchi 富山大学, 大学院・医学薬学研究部(薬学), 准教授 (40303234)
MAMORU Fukuchi 富山大学, 大学院・医学薬学研究部(薬学), 助教 (40432108)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 遺伝子発現 / シナプス / BDNF / PACAP / CREB / MKL |
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| Research Abstract |
Since G protein-coupled receptors (GPCRs) potentiate the activity of the N-methyl-D-aspartate receptor (NMDA-R), we comprehensively investigated gene expression induced by the stimulation of GPCRs in culture. Potentiation of NMDA-R mediated by the activation of PAC1, a GPCR, with PACAP immediately activated a limited number of genes upon Ca^<2+> signals. The mRNA expression of the brain-derived neurotrophic factor exon IV-IX (Bdnf-eIV) is totally controlled by Ca^<2+>/calcineurin, leading to the CRE/CREB-dependent transcription. Stimulation of NMDA-R under the activation of PAC1 synergistically increased Bdnf-eIV mRNA expression through the selective activation of calcineurin, leading to translocation of TORC1 to nucleus and activation of CREB-KID domain. The Ca^<2+>/calcineurin-dependent mRNA expression was also induced by direct stimulation of PKA or PKC pathway and, furthermore, by that of the dopamine D1 or β-adrenergic receptor. Thus, the potentiation of NMDA-R activity mediated by GPCR induces activity-dependent gene expression through calcineurin pathway, possibly regardless of the type of neurotransmission.
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Research Products
(44 results)
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[Journal Article] Antibody to varicella-zoster virus immediate-early protein 62 augments allodynia in zoster via brain-derived neurotrophic factor.2010
Author(s)
Hama Y., Shiraki K., Yoshida Y., Maruyama A., Yasuda M., Tsuda M., Honda M., Takahashi M., Higuchi H., Takasaki I., Daikoku T., Tsumoto T.
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Journal Title
J.Virol. 84
Pages: 1616-1624
Peer Reviewed
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