Functional non-coding RNA expression in activated hepatic stellate cells

2010 Fiscal Year Final Research Report

• Project/Area Number: 20390053
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: Nagoya City University
• Principal Investigator: IKEDA Kazuo Nagoya City University, 大学院・医学研究科, 教授 (80275247)
• Project Period (FY): 2008 – 2010
• Keywords: 細胞分化・組織形成 / 肝星細胞 / マイクロRNA / 肝線維化 / コラーゲン

Research Abstract

MicroRNA, which are endogenous small non-coding RNA, have recently become a focus of interest as post-transcriptional regulators of gene expression. Using a microRNA array and Applied Biosystem's Taq Man micro-RNA assay, we profiled microRNA expression in activated stellate cells and fibrotic liver. More than 20 microRNAs changed their expression levels in activated stellate cells and in fibrotic liver. Among them, miR-17-92 were closely related to cell's proliferation and the liver injury. miR-200b had an association with epithelial-mesenchymal transition.

Research Products

• [Journal Article] Down-regulation of cyclin E1 expression by micro RNA-195 accounts for interferon-beta-induced inhibition of hepatic stellate cell proliferation. (2010)
  • Author(s): Sekiya Y, Ogawa T, Iizuka M, Yoshizato K, Ikeda K, Kawada N.
  • Journal Title: J Cell Physiol (Epub ahead of print)
  • Peer Reviewed
• [Journal Article] Suppression of type I collagen production by micro RNA-29b in cultured human stellate cells. (2010)
  • Author(s): Ogawa T, Yoshizato K, Ikeda K, 他
  • Journal Title: Biochem Biophys Res Commun 1;391(1) Pages: 316-21
  • Peer Reviewed
• [Journal Article] Laminin-511, inducer of hairgrowth, is down-regulated and its suppressor in hair growth, laminin-332 up-regulated in chemotherapy-induced alopecia. (2010)
  • Author(s): Imanishi H, Tsuruta D, Ikeda K, 他
  • Journal Title: J Dermatol Sci 58(1) Pages: 43-54
  • Peer Reviewed
• [Journal Article] Dynamic relationship of focal contacts and hemidesmosome protein complexes in live cells. (2010)
  • Author(s): Ozawa T, Tsuruta D, Ikeda K, 他
  • Journal Title: J Invest Dermatol 130(6) Pages: 1624-35
  • Peer Reviewed
• [Journal Article] Induction of tropomyosin during hepatic stellate cell activation and the progression of liver fibrosis. (2009)
  • Author(s): Otogawa K, Ikeda K, 他
  • Journal Title: Hepatol Int 3(2) Pages: 378-83
  • Peer Reviewed
• [Journal Article] Effect of natural interferon alpha on proliferation and apoptosis of hepatic stellate cells. (2009)
  • Author(s): Ogawa T, Kawada N, Ikeda K.
  • Journal Title: Hepatol Int 3(3) Pages: 497-503
  • Peer Reviewed
• [Journal Article] Impaired cutaneous wound healing with excess granulation tissue formation in TNFalpha-null mice. (2009)
  • Author(s): Shinozaki M, Ikeda K, 他
  • Journal Title: Arch Dermatol Res 301(7) Pages: 531-537
  • Peer Reviewed
• [Journal Article] Negligible contribution of bonemarrow-derived cells to collagen production during hepatic fibrogenesis in mice. (2009)
  • Author(s): Higashiyama R, Ikeda K, Inagaki Y., 他
  • Journal Title: Gastroenterology 137(4) Pages: 1459-1466.e1
  • Peer Reviewed
• [Journal Article] Suppression of injury-induced conjunctiva scarring by peroxisome proliferator-activated receptor gamma gene transfer in mice. (2009)
  • Author(s): Yamanaka O, Ikeda K., 他
  • Journal Title: Invest Ophthalmol Vis Sci 50(1) Pages: 187-93
  • Peer Reviewed
• [Presentation] マウス肝臓発生過程におけsyntaxin 2の発現と肝芽細胞の増殖・分化に対する影響 (2010)
  • Author(s): 杉山良典、富谷智明、池田一雄、小池亨、塩尻信義
  • Organizer: 第17回肝細胞研究会
  • Place of Presentation: 秋田アトリオン
  • Year and Date: 2010-06-19
• [Presentation] I 型インターフェロンのマイクロRNA発現調節を介した肝星細胞増殖抑制作用 (2010)
  • Author(s): 関谷由美子, 小川智弘, 飯塚昌司, 吉里勝利, 池田一雄, 河田則文
  • Organizer: 第46回日本肝臓学会総会
  • Place of Presentation: 山形
  • Year and Date: 2010-05-27
• [Presentation] 肝星細胞活性化におけるマイクロPNAの関与についての検討 (2009)
  • Author(s): 陳輝, 小川智之, 飯塚昌司, 関谷由美子, 諏訪友紀子, 上田優希子, 庄秋栄, 葛岩, 後藤公寿, 河田則文, 池田一雄
  • Organizer: 第23回肝類洞壁細胞研究会
  • Place of Presentation: 大阪
  • Year and Date: 2009-12-12
• [Presentation] 星細胞のコラーゲン発現に関与するマイクロRNA とその機能 (2009)
  • Author(s): 飯塚昌司, 小川智之, 関谷由美子, 吉里勝利, 池田一雄, 河田則文
  • Organizer: 第23回肝類洞壁細胞研究会
  • Place of Presentation: 大阪
  • Year and Date: 2009-12-12
• [Presentation] 肝疾患の治療を目的とするドラッグデリバリーシステムの開発と形態学。解剖学雑誌2009;84 巻S18-2, Page107 (2009)
  • Author(s): 金井美晴、村田義夫、池田一雄、曽爾彊
  • Organizer: 第114回日本解剖学会総会
  • Place of Presentation: 岡山
  • Year and Date: 2009-03-30