Regulation of intracellular localization of ENaC by protease

2011 Fiscal Year Final Research Report

• Project/Area Number: 20390060
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General physiology
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: MARUNAKA Yoshinori 京都府立医科大学, 大学院・医学研究科, 教授 (00127036)
• Co-Investigator(Kenkyū-buntansha): NIISATO Naomi 京都府立医科大学, 医学研究科, 准教授 (00237645) ; NAKAJIMA Ken-ichi 京都府立医科大学, 医学研究科, 助教 (40398392) ; KUSUZAKI Katsuyuki 平安女学院, 文化創造センター, 客員教授 (30177993) ; ASHIHARA Eishi 京都府立医科大学, 医学研究科, 講師 (70275197) ; HOSOGI Shigekuni 京都府立医科大学, 医学研究科, 助教 (30405261)
• Project Period (FY): 2008 – 2011
• Keywords: ENaC / アルデステン / 皮質集合管 / プロテアーゼ阻害剤 / 浸透圧 / トラッフィッキング / 高血圧 / ナトリウム輸送

Research Abstract

We tried to clarify the regulation of intracellular localization of ENaC by protease. We have found that : 1) aldosterone increases expression of protease, and 2) aldosterone elevates the recycling efficiency of ENaC in the intracellular space by cleavage of ENaC mediated through protease.

Research Products

• [Journal Article] Hypotonic stress upregulates beta-and gamma-ENaC expression through suppression of ERK by inducing MKP-1 (2012)
  • Author(s): Niisato N, Ohta M, Eaton DC, Marunaka Y
  • Journal Title: Am J Physiol Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Sensing mechanism of stretch activated ion channels Mechanically Gated Channels and mechanisms of their regulation/. Mechanosensitivity in Cells and Tissues. (2012)
  • Author(s): Niisato N, Marunaka Y.
  • Journal Title: Springer Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Procaterol-stimulated increases in ciliary bend amplitude and ciliary beat frequency in mouse bronchioles. (2012)
  • Author(s): Komatani-Tamiya N, Daikoku E, Takemura Y, Shimamoto C, Nakano T, Iwasaki Y, Matsumura H, Marunaka Y, Nakahari T
  • Journal Title: Cell Physiol Biochem Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Regulation of epithelial sodium transport via epithelial Na^+channel(ENaC) (2011)
  • Author(s): MARUNAKA Y, Niisato N, Taruno A, Ohta M, Miyazaki H, Hosogi S, Nakajima K, Kusuzaki K, Ashihara E, Nishio K, Iwasaki Y, Nakahari T, Kubota T
  • Journal Title: J Biomed Biotechnol Volume: 2011 Pages: 978196
  • Peer Reviewed
• [Journal Article] Quercetin stimulates NGF-induced neurite outgrowth in PC12 cells via activation of Na^+/K^+/2Cl^-cotransporter (2011)
  • Author(s): Nakajima K, Niisato N, MARUNAKA Y
  • Journal Title: Cell Physiol Biochem Volume: 28 Pages: 147-156
  • Peer Reviewed
• [Journal Article] Analysis of blocker-labeled channels reveals the dependence of recycling rates of ENaC on the total amount of recycled channels (2010)
  • Author(s): Taruno A, Marunaka Y
  • Journal Title: Cell Phsyiol Biochem Volume: 26巻 Pages: 925-934
  • Peer Reviewed
• [Journal Article] PPAR-alpha modulation of Ca^<2+>-regulated exocytosis via arachidonic acid in guinea pig antral mucous cells (2010)
  • Author(s): Sawabe Y, Shimamoto C, Sakai A, Kuwabara H, Saad AH, Nakano T, Takitani K, Tamai H, Mori H, MARUNAKA Y, Nakahari T
  • Journal Title: Exp Physiol Volume: 95 Pages: 858-868
  • Peer Reviewed
• [Journal Article] NaCl flux between apical and basolateral side recruits claudin-1 to tight junction strands and regulate paracellular transport (2010)
  • Author(s): Tokuda S, Miyazaki H, Nakajima K, Yamada T, MARUNAKA Y
  • Journal Title: Biochem Biophys Res Commun Volume: 393 Pages: 390-396
  • Peer Reviewed
• [Journal Article] Hydrostatic pressure regulates tight junctions, actin cytoskeleton and transcellular on transport (2009)
  • Author(s): Tokuda S, Miyazaki H, Nakajima K, Yamada T, MARUNAKA Y
  • Journal Title: Biochem Biophys Res Commun Volume: 390 Pages: 1315-1321
  • Peer Reviewed
• [Journal Article] Regulation of paracellular Na^+and Cl^-conductances by hydrostatic pressure (2009)
  • Author(s): Tokuda S, Niisato N, Nagai T, Taruno A, Nakajima K, Miyazaki H, Yamada T, Hosogi S, Ohta M, Nishio K, Iwasaki Y, MARUNAKA Y
  • Journal Title: Cell Biol Int Volume: 33 Pages: 949-956
  • Peer Reviewed
• [Journal Article] Effects of extracellular chloride ion on epithelial sodium channel(ENaC) in arginine vasotocin(AVT)-stimulated renal epithelial cells (2009)
  • Author(s): Yamada T, Niisato N, MARUNAKA Y
  • Journal Title: Biomed Res Volume: 30 Pages: 193-198
  • Peer Reviewed
• [Journal Article] Regulation of the paracellular Na^+and Cl^-conductances by the NaCl-generated osmotic gradient in a manner dependent on the direction of osmotic gradients (2008)
  • Author(s): Tokuda S, Niisato N, Nakajima K, MARUNAKA Y
  • Journal Title: Biochem Biophys Res Commun Volume: 366 Pages: 464-470
  • Peer Reviewed
• [Journal Article] Chloride ions control the G1/S cell-cycle checkpoint by regulating the expression of p21 through a p53-independent pathway in human gastric cancer cells (2008)
  • Author(s): Miyazaki H, Shiozaki A, Niisato N, Ohsawa R, Itoi H, Ueda Y, Otsuji E, Yamagishi H, Iwasaki Y, Nakano T, Nakahari T, MARUNAKA Y
  • Journal Title: Biochem Biophys Res Commun Volume: 366 Pages: 506-512
  • Peer Reviewed
• [Journal Article] Intracellular calcium plays a role as the second messenger of hypotonic stress in gene regulation of SGK1 and ENaC in renal epithelial A6 cells (2008)
  • Author(s): Taruno A, Niisato N, MARUNAKA Y
  • Journal Title: Am J Physiol Renal Physiol Volume: 294 Pages: F177-186
  • Peer Reviewed
• [Journal Article] Osmortransduction through volume-sensitive Cl-channels. Mechanosensitive ion channels/Mechanosensitivity in Cells and Tissues (2008)
  • Author(s): Niisato N, MARUNAKA Y
  • Journal Title: Springer Volume: Vol.1 Pages: 179-202
  • Peer Reviewed
• [Presentation] Recycling rates of ENaC depend on the total amount of recycled channels (2012)
  • Author(s): Marunaka Y, Taruno A
  • Organizer: The 89^<th> Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: Matsumoto
  • Year and Date: 20120329-31
• [Presentation] Hypotonicity-induced upregulation of beta-and gamma-ENaC expression through suppression of ERK by inducing MKP-1 (2011)
  • Author(s): Marunaka Y, Niisato N, Ohta M
  • Organizer: The 7th International Symposium on Aldosterone and the ENaC/Degenerin Family of Ion Channels : Molecular Mechanisms and Pathophysiology
  • Place of Presentation: Pacific Grove, California, USA
  • Year and Date: 20110918-22
• [Remarks]
  • URL: http://kpum-molecular-cell-physiology.info/index.html