Visualizing the dynamics of senescence response in vivo

2010 Fiscal Year Final Research Report

• Project/Area Number: 20390094
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pathological medical chemistry
• Research Institution: Japanese Foundation For Cancer Research
• Principal Investigator: HARA Eiji Japanese Foundation For Cancer Research, 癌研究所がん生物部, 部長 (80263268)
• Project Period (FY): 2008 – 2010
• Keywords: 癌 / 老化 / イメージング / シグナル伝達 / ストレス

Research Abstract

Cellular senescence has long been considered as an important mechanism controlling tumor suppression and aging. However because cellular senescence had been studied mainly in vitro, it was unclear whether cellular senescence indeed occurs in vivo. To circumvent this problem, here we developed a system to visualize the dynamics of senescence response in vivo, especially in the context of living animal. This approach, in comjunction with the knockout mice studies, unveils biological roles of cellular senescence in vivo.

Research Products

• [Journal Article] Reversion-inducing cysteine-rich protein with Kazal motifs (RECK) interferes with epidermal growth factor receptor (EGFR) signaling. (2011)
  • Author(s): Kitajima S., Miki T., Takegami Y., Kido Y., Noda M., Hara E., Shamma A., Takahashi C.
  • Journal Title: Oncogene 30 Pages: 737-750
• [Journal Article] Intrinsic cooperation between p16^<INK4a> and p21^<Waf1/Cip1> in the onset of cellular senescence and tumor suppression in vivo. (2010)
  • Author(s): Takeuchi S., Takahashi A., Motoi N., Yoshimoto S., Tajima T., Yamakoshi K., Hirao A., Yanagi S., Fukami K., Ishikawa Y., Sone S., Hara E., Ohtani N.
  • Journal Title: Cancer Res. 70 Pages: 9381-9390
• [Journal Article] Normal proliferation and tumorigenesis but impaired pancreatic function in mice lacking the cell cycle regulator seil. (2010)
  • Author(s): Fernandez-Marcos P.J., Pantoja C., Gonzalez-Rodriguez A., Martin N., Flores J.M., Valverde A.M., Hara E., Serrano M.
  • Journal Title: PLoS One. 18 Pages: e8744
• [Journal Article] Real-time in vivo imaging of p16^<Ink4a> reveals cross-talk with p53. (2009)
  • Author(s): Yamakoshi K., Takahashi A., Hirota F., Nakayama R., Ishimaru N., Kubo Y., Mann D.J., Ohmura M., Hirao A., Saya H., Arase S., Hayashi Y., Nakao K., Matsumoto M., Ohtani N., Hara E.
  • Journal Title: J. Cell Biol. 186 Pages: 393-407
• [Journal Article] Genotoxic stress abrogates renewal of melanocyte stem cells by triggering their differentiation. (2009)
  • Author(s): Inomata K., Aoto T., Binh N.T., Okamoto N., Tanimura S., Wakayama T., Iseki S., Hara E., Masunaga T., Shimizu H., Nishimura E.K.
  • Journal Title: Cell 137 Pages: 1088-1099
• [Presentation] 蛍光・発光融合プローブを用いたOct4遺伝子イメージングシステムの開発 (2010)
  • Author(s): 田島丈子、杉田千鶴、近江谷克祐、中尾和貴、柳茂、深見希代子、原英二、大谷直子
  • Organizer: 日本分子生物学会・日本生化学会合同年会
  • Place of Presentation: 神戸
  • Year and Date: 20101207-20101210
• [Presentation] Backup tumor suppressor role for p16^<INK4a> in the event of p53 inactivation (2010)
  • Author(s): Takahashi A., Yamakoshi K., Ohtani N., Hara E
  • Organizer: 日本分子生物学会・日本生化学会合同年会
  • Place of Presentation: 神戸
  • Year and Date: 20101207-20101210
• [Presentation] DNA damage dependent degradation of histone methyltransferase in cellular senescence. (2010)
  • Author(s): Takahashi A., Yamakoshi K., Imai Y., Ohtani N., Hara E
  • Organizer: Cold Spring Harbor Laboratory Meeting : Molecular Genetics of Aging
  • Place of Presentation: New York, U.S.A.
  • Year and Date: 20100928-20101002
• [Presentation] Intrinsic cooperation between p16^<INK4a> and p21^<Waf1/Cip1> in the onset of cellular senescence and tumor suppression in vivo. (2010)
  • Author(s): Takeuchi S., Takahashi A., Motoi N., Yoshimoto S., Tajima T., Yamakoshi K., Ishikawa Y., Sone S., Hara E., Ohtani N.
  • Organizer: Cold Spring Harbor Laboratory Meeting : Molecular Genetics of Aging
  • Place of Presentation: New York, U.S.A.
  • Year and Date: 20100928-20101002
• [Presentation] 細胞老化において発癌を促進する因子の探索 (2010)
  • Author(s): 今井良紀、高橋暁子、八尾良司、大谷直子、広田亨、田原栄俊、井出利憲、野田哲生、原英二
  • Organizer: 日本癌学会
  • Place of Presentation: 大阪
  • Year and Date: 20100922-20100924
• [Presentation] Real-time in vivo imaging of p16^<Ink4a> reveals cross-talk with p53. (2010)
  • Author(s): 原英二
  • Organizer: IMCB symposium on cell cycle regulation and tumorigenesis.
  • Place of Presentation: Singapore, Singapore
  • Year and Date: 20100907-20100908
• [Presentation] Cellular senescence: its role in tumor suppression and aging (2010)
  • Author(s): 原英二
  • Organizer: Asian aging core for longevity research
  • Place of Presentation: Jeju, Korea
  • Year and Date: 20100822-20100824
• [Presentation] 細胞老化の分子メカニズムとその役割 (2010)
  • Author(s): 原英二
  • Organizer: 日本炎症・再生医学会
  • Place of Presentation: 東京
  • Year and Date: 20100805-20100806
• [Presentation] Real-time in vivo imaging of p16^<Ink4a> expression unveils a cross-talk between p53 and p16^<Ink4a> (2009)
  • Author(s): Yamakoshi K., Takahashi A., Ohtani N., Hara E
  • Organizer: The Salk Institute Meeting /Mechanisms & Models of Cancer
  • Place of Presentation: La Jolla, U.S.A.
  • Year and Date: 20090812-20090816
• [Presentation] Visualizing the dynamics of oncogenic stress response in living mice. (2009)
  • Author(s): Ohtani N., Yamakoshi K., Takahashi A., Hara E
  • Organizer: AACR Conference on Mouse model of Cancer
  • Place of Presentation: San Francisco, U.S.A.
  • Year and Date: 20090112-20090115
• [Presentation] Real-time imaging of p16^<INK4a> expression visualizes the dynamics of senescence signalling in living animals. (2008)
  • Author(s): Yamakoshi K., Takahashi A., Ohtani N., Hara E
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神戸
  • Year and Date: 20081209-20081212
• [Book] がん分子標的治療研究マニュアル(鶴尾隆/曽根三郎編)(細胞周期アッセイ法) (2009)
  • Author(s): 高橋暁子、原英二
  • Publisher: 金芳堂
• [Remarks] ホームページ等
  • URL: http://www.jfcr.or.jp/tci/canbio/