Regulation of acquired immunity via negative feedback mechanism between dendritic cells and NK-T cells

2010 Fiscal Year Final Research Report

• Project/Area Number: 20390106
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Experimental pathology
• Research Institution: Hokkaido University
• Principal Investigator: IWABUCHI Kazuya Hokkaido University, 医学部, 教授 (20184898)
• Co-Investigator(Kenkyū-buntansha): ONO Kazunori 北海道大学, 遺伝子病制御研究所, 教授 (40002117) ; YANAGAWA Yoshiki 北海道医療大学, 薬学部, 講師 (20322852)
• Project Period (FY): 2008 – 2010
• Keywords: 樹状細胞 / NK-T細胞 / ネガティブフィードバック / 免疫制御 / 病態モデル / 生活習慣病 / 動脈硬化症 / 自然免疫

Research Abstract

We demonstrated that balances of cytokines are negatively or positively regulated in dendritic cells with a unique combination such as IL-12/23 production by prostaglandin E2, IL-10 by TNF-α, and IL-33 by noradrenalin. We also found that NK-T cells (especially type II subset) play an accelerating role in visceral fat syndrome. In atherosclerotic model, CD1d-restricted NKT cell subset seemed to be negatively regulated by MR1-restricted NKT cell subset.

Research Products

• [Journal Article] CSF-1-dependent red pulp macro phages regulate CD4 T cell responses. (2011)
  • Author(s): Kurotaki D, Kon S, Bae K, Ito K, Matsui Y, Nakayama Y, Kanayama M, Kimura C, Narita Y, Nishimura T, Iwabuchi K, Mack M, van Rooijen N, Sakaguchi S, Uede T, Morimoto J.
  • Journal Title: J Immunol 186(4) Pages: 2229-2237
  • Peer Reviewed
• [Journal Article] The role of tumor necrosis factor-α for interleukin-10 production by murine dendritic cells. (2011)
  • Author(s): Hirata N, Yanagawa Y, Ogura H, Satoh M, Noguchi M, Matsumoto M, Togashi H, Onoe K, Iwabuchi K.
  • Journal Title: Cell Immunol 266 Pages: 165-171
  • Peer Reviewed
• [Journal Article] Identification of INAM, a polyI:C-inducible membrane protein, that participates in dendritic cellmediated natural killer cell activation. (2010)
  • Author(s): Ebihara T, Azuma M, Oshiumi H, Kasamatsu J, Iwabuchi K, Matsumoto K, Saito H, Taniguchi T, Matsumoto M, Seya T.
  • Journal Title: J Exp Med 207(12) Pages: 2675-2687
  • Peer Reviewed
• [Journal Article] Enhanced dendritic cell antigen uptake via α2 adrenoreceptor-mediated PI3K activation following brief exposure to noradrenaline. (2010)
  • Author(s): Yanagawa Y, Matsumoto M, Togashi H.
  • Journal Title: J Immunol 185(10) Pages: 5762-5768
  • Peer Reviewed
• [Journal Article] Identification of a third variable lymphocyte receptor in the lamprey. (2010)
  • Author(s): Kasamatsu J, Sutoh Y, Fugo K, Otsuka N, Iwabuchi K, Kasahara M.
  • Journal Title: Proc Natl Acad Sci USA 107(32) Pages: 14304-14308
  • Peer Reviewed
• [Journal Article] Differential enhancement of T helper type 1 (Th1)/Th2 cytokine production by natural killer T cells through negative feedback regulation with pre-treated dendritic cells. (2010)
  • Author(s): Iwabuchi K, Yanagawa Y, Minami K, Mizuuchi K, Maeda K, Ogura H, Satou S, Iwata D, Hirata H, Iwabuchi C, Onoe K.
  • Journal Title: Curr Immunol Rev 6(2) Pages: 78-87
  • Peer Reviewed
• [Journal Article] Dendritic cell differentiation with prostaglandin E2 results in selective attenuation of the ERK pathway and decreased interleukin-23 production. (2010)
  • Author(s): Hayashi F, Yanagawa Y, Onoe K, Iwabuchi K.
  • Journal Title: Immunology 131(1) Pages: 67-76
  • Peer Reviewed
• [Journal Article] Nuclear factor-κB (NF-κB) inhibitor, Dehydroxymethylepoxyquinomicin( DHMEQ) ameliorates experimental autoimmune uveoreitnitis (EAU) in mice. (2010)
  • Author(s): Iwata D, Kitaichi N, Ebihara A, Iwabuchi K, Yoshida K, Namba K, Ozaki M, Ohno S, Umezawa K, Yamashita K-i, Todo S, Ishida S, Onoe K.
  • Journal Title: Invest Ophthalmol Vis Sci 51(4) Pages: 2077-2084
  • Peer Reviewed
• [Journal Article] Dendritic cellderived TNF-α is responsible for development of IL-10-producing CD4^+ T cells. (2010)
  • Author(s): Hirata N, Yanagawa Y, Satoh M, Ogura H, Ebihara T, Noguchi M, Matsumoto M, Togashi H, Seya T, Onoe K, Iwabuchi K.
  • Journal Title: Cell Immunol 261(1) Pages: 37-41
  • Peer Reviewed
• [Journal Article] Natural killer T cells are involved in adipose tissue inflammation and glucose intolerance in diet-induced obese mice. (2010)
  • Author(s): Ohmura K, Ishimori N, Ohmura Y, Tokuhara S, Nozawa A, Hori S, Andoh Y, Fujii S, Iwabuchi K, Onoe K, Tsutsui H.
  • Journal Title: Arterioscler Thromb Vasc Biol 30 Pages: 193-199
  • Peer Reviewed
• [Journal Article] Prevention of experimental autoimmune uveoretinitis by blockade of osteopontin with small interfering RNA. (2010)
  • Author(s): Iwata D, Kitamura M, Kitaichi N, Saito Y, Kon S, Morimoto J, Miyazaki A, Kitamei H, Namba K-i, Yoshida K, Ohno S, Uede T, Onoe K, Iwabuchi K.
  • Journal Title: Exp Eye Res 90(1) Pages: 41-48
  • Peer Reviewed
• [Journal Article] Selective regulation of interleukin-10 production via Janus kinase pathway in murine conventional dendritic cells. (2009)
  • Author(s): Hirata N, Yanagawa Y, Iwabuchi K, Onoe K.
  • Journal Title: Cell Immunol 258 Pages: 9-17
  • Peer Reviewed
• [Journal Article] Expansion of CD4^+CD28^- T cells producing high levels of interferon-γ in peripheral blood of patients with multiple sclerosis. (2008)
  • Author(s): Miyazaki Y, Iwabuchi K, Iwata D, Miyazaki A, Kon Y, Niino M, Kikuchi S, Yanagawa Y, Van Kaer L, Sasaki H, Onoe K.
  • Journal Title: Multiple Sclerosis 14 Pages: 1044-1055
  • Peer Reviewed
• [Journal Article] Osteopontin regulates development and function of invariant natural killer T cells. (2008)
  • Author(s): Diao H, Iwabuchi K, Li L, Onoe K, Van Kaer L, Kon S, Saito Y, Morimoto J, Denhardt D, Rittling S, Uede T.
  • Journal Title: Proc Natl Acad Sci USA 105 Pages: 15884-15889
  • Peer Reviewed
• [Journal Article] Selective synergy in anti-inflammatory cytokine production upon cooperated signaling via TLR4 and TLR2 in murine conventional dendritic cells. (2008)
  • Author(s): Hirata N, Yanagawa Y, Ebihara T, Seya T, Uematsu S, Akira S, Hayashi F, Iwabuchi K, Onoe K.
  • Journal Title: Mol Immunol 45(10) Pages: 2734-2742
  • Peer Reviewed
• [Journal Article] In vitro induction of natural killer T cells from embryonic stem cells prepared by somatic cell nuclear transfer. (2008)
  • Author(s): Wakao H, Wakao R, Sakata S, Iwabuchi K, Oda A, Fujita H.
  • Journal Title: FASEB J 22(7) Pages: 2223-22231
  • Peer Reviewed
• [Journal Article] Cooperative action of IL-10 and IFN-γ to regulate dendritic cell function. (2008)
  • Author(s): Yanagawa Y, Iwabuchi K, Onoe K.
  • Journal Title: Immunology 127 Pages: 345-353
  • Peer Reviewed
• [Journal Article] Adrenoreceptor-mediated enhancement of interlekin-33 production by dendritic cells.
  • Author(s): Yanagawa Y, Matsumoto M, Togashi H.
  • Journal Title: Brain Behav Immun. in press
  • Peer Reviewed
• [Presentation] Amelioration of experimental autoimmune uveoretinitis by blockade of osteopontin with antibody or small interfering RNA. (2010)
  • Author(s): Iwabuchi K, Iwata D, Kitamura M, Kitaichi N, Kon S, Kitamei H, Namba K, Yoshida K, Ishida S, Ohno S, Rittling SR, Denhardt DT, Uede T, Onoe K.
  • Organizer: 14^<th> International Congress of Immunology
  • Place of Presentation: Kobe
  • Year and Date: 2010-08-27
• [Presentation] Selective attenuation of the ERK pathway and decreased interleukin-23 production in dendritic cells differentiated with prostaglandin E2. (2010)
  • Author(s): Hayashi F, Yanagawa Y, Onoe K, Iwabuchi K
  • Organizer: 14^<th> International Congress of Immunology
  • Place of Presentation: Kobe
  • Year and Date: 2010-08-25
• [Presentation] The role of dendritic cell-produced TNF-α in generation of IL-10-producing CD4^+ T cells. (2010)
  • Author(s): Hirata N, Yanagawa Y, Iwabuchi K, Satoh M, Ogura H, Seya T, Onoe K, Noguchi M.
  • Organizer: 14^<th> International Congress of Immunology
  • Place of Presentation: Kobe
  • Year and Date: 2010-08-24
• [Presentation] Type II NKT cells operate diet-induced obesity. (2010)
  • Author(s): Satoh M, Ogura H, Andoh Y, Nakayama T, Tanihuchi M, Onoe K, Iwabuchi K
  • Organizer: 14^<th> International Congress of Immunology
  • Place of Presentation: Kobe
  • Year and Date: 2010-08-23
• [Presentation] MR1-restricted NKT cells exhibit beneficial role for the development of atherosclerosis. (2010)
  • Author(s): Ogura H, Satoh M, Gilfillan S, Miyake S, Onoe K, Iwabuchi K
  • Organizer: 14^<th> International Congress of Immunology
  • Place of Presentation: Kobe
  • Year and Date: 2010-08-23
• [Presentation] 生活習慣病マウスモデルにおけるCD1d拘束性とMR1拘束性NKT細胞の異なる機能発現 (2010)
  • Author(s): 岩渕和也・佐藤雅・小倉尚子・小野江和則・Susan Gilfillan, Luc Van Kaer・島村道夫・三宅幸子・山村隆
  • Organizer: 第20回KTCC
  • Place of Presentation: 京都
  • Year and Date: 2010-06-05
• [Presentation] Further characterization of NKT-cell hybridomas with invariant T-cell antigen receptor. (2009)
  • Author(s): Obata Y, Iwabuchi K.
  • Organizer: The 1st International Young Researcher Seminar in Zoonosis Control.
  • Place of Presentation: ニセコ
  • Year and Date: 20090819-20090821
• [Presentation] Cooperative effects of IL-10 and IFN-γ on dendritic cell functions. (2009)
  • Author(s): Yanagawa Y, Iwabuchi K, Matsumoto M, Onoe K, Togashi H.
  • Organizer: 第39回日本免疫学会総会・学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2009-12-04
• [Presentation] Effect of prostaglandin E2 on IL-23 production and ERK activity in conventional dendritic cells. (2009)
  • Author(s): Hayashi F, Yanagawa Y, Onoe K, Iwabuchi K.
  • Organizer: 第39回日本免疫学会総会・学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2009-12-04
• [Presentation] JAK-PI3K pathway operates IL-10 production in murine dendritic cells. (2009)
  • Author(s): Hirata N, Yanagawa Y, Iwabuchi K, Noguchi M, Onoe K.
  • Organizer: 第39回日本免疫学会総会・学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2009-12-04
• [Presentation] Enhanced development of atherosclerosis in [β2m-/--> Ldlr-/-] irradiation bone-marrow chimeras. possible involvement of innate T cell other than CD1d-restricted NKT cells. (2009)
  • Author(s): Ogura H, Satoh M, Van Kaer L, Nakayama T, Taniguchi M, Onoe K, Iwabuchi K.
  • Organizer: 第39回日本免疫学会総会・学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2009-12-02
• [Presentation] Diet-induced obesity is suppressed in CD1d-deficient but not in invariant NKT cell-deficient mice. (2009)
  • Author(s): Satoh M, Ogura H, Andoh, Ishimori N, Fujii S, Nakayama T, Taniguchi M, Van Kaer L, Onoe K, Iwabuchi K.
  • Organizer: 第39回日本免疫学会総会・学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2009-12-02
• [Presentation] Administration of osteopontin small interfering RNA ameliorates experimental autoimmune uveoretinitis model in mice. (2009)
  • Author(s): Iwabuchi K, Iwata D, Kitamura M, Saito Y, Kon S, Morimoto J, Ohno S, Ishida S, Uede T, Onoe K.
  • Organizer: 第9回オステオポンチン研究会
  • Place of Presentation: 札幌
  • Year and Date: 2009-09-13
• [Presentation] NKT細胞の機能と生活習慣病の進展における役割 (2009)
  • Author(s): 岩渕和也
  • Organizer: 第15回福岡大学グローバルFUプログラムセミナー
  • Place of Presentation: 福岡
  • Year and Date: 2009-07-16
• [Presentation] 食餌誘導性肥満進展におけるNKT細胞の役割 (2009)
  • Author(s): 岩渕和也, 小野江和則
  • Organizer: 第99回日本病理学会総会
  • Place of Presentation: 東京
  • Year and Date: 2009-04-29
• [Presentation] NKT cells control the volume of visceral fat in mice fed high fat diet (HFD). (2009)
  • Author(s): Satoh M, Iwabuchi K, Ogura H, Onoe K.
  • Organizer: The 5^<th> Intl Symposium on CD1/NKT cells.
  • Place of Presentation: Kamakura, Japan
  • Year and Date: 2009-03-24
• [Presentation] A collaborative role of NK cells with NKT cells in the lipopolysaccharide-induced aggravation of atherosclerosis in apolipoprotein E knockout mice. (2009)
  • Author(s): Ogura H, Andoh Y, Iwabuchi K, Satoh M, Fujii S, Onoe K.
  • Organizer: The 5^<th> Intl. Symposium on CD1/NKT cells.
  • Place of Presentation: Kamakura, Japan
  • Year and Date: 2009-03-24
• [Presentation] Involvement of allograft inflammatory factor-1 for the development of atherosclerosis in murine model. (2008)
  • Author(s): Satoh M, Iwabuchi K, Ogura H, Fujii S, Onoe K.
  • Organizer: 第38回日本免疫学会総会・学術集会
  • Place of Presentation: 京都
  • Year and Date: 2008-12-03
• [Presentation] TLR4 acts in synergy with TLR2 in IL-10 production by murine dendritic cells. (2008)
  • Author(s): Hirata N, Yanagawa Y, Ebihara T, Seya T, Uematsu S, Akira S, Hayashi F, Iwabuchi K, Onoe K.
  • Organizer: 第38回日本免疫学会総会・学術集会
  • Place of Presentation: 京都
  • Year and Date: 2008-12-02
• [Presentation] A collaborative role of NK cells with NKT cells in the aggravation of artherosclerosis induced by lipopolysaccharide administration. (2008)
  • Author(s): Ogura H, Andoh Y, Iwabuchi K, Satoh M, Fujii S, Onoe K.
  • Organizer: 第38回日本免疫学会総会・学術集会
  • Place of Presentation: 京都
  • Year and Date: 2008-12-01
• [Presentation] A演説ナチュラルキラーT(NKT)細胞機能の分化と生体内における新しい役割 (2008)
  • Author(s): 岩渕和也
  • Organizer: 第54回日本病理学会秋期特別総会
  • Place of Presentation: 松山
  • Year and Date: 2008-11-21
• [Presentation] 新規NF-κB阻害薬DHMEQによる免疫抑制樹状細胞の誘導 (2008)
  • Author(s): 青柳武史, 山下健一郎, 柳川芳毅, 後藤了一, 五十嵐瑠美, 芳賀さなえ, 尾崎倫孝, 小野江和則, 梅澤一夫, 藤堂省
  • Organizer: 第48回日本リンパ網内系学会総会
  • Place of Presentation: 札幌
  • Year and Date: 2008-06-14
• [Presentation] TLR4とTLR2を介した選択的かつ相乗的な抗炎症性サイトカインの産生 (2008)
  • Author(s): 平田徳幸, 柳川芳毅, 海老原敬, 瀬谷司, 植松智, 審良静男, 林史恵, 岩渕和也, 小野江和則
  • Organizer: 第48回日本リンパ網内系学会総会
  • Place of Presentation: 札幌
  • Year and Date: 2008-06-14
• [Presentation] シンポジウム樹状細胞研究の新展開と樹状細胞免疫療法の現状と将来「樹状細胞におけるサイトカイン産生バランスの制御機構」 (2008)
  • Author(s): 柳川芳毅, 平田徳幸, 小野江和則
  • Organizer: 第48回日本リンパ網内系学会総会
  • Place of Presentation: 札幌
  • Year and Date: 2008-06-13
• [Remarks] ホームページ等
  • URL: http://web.med.kitasato-u.ac.jp/edures/immunol.html