2010 Fiscal Year Final Research Report
Resistance genes to collagen disease in a wild mice-derived inbred strain MSM/Ms
| Project/Area Number |
20390112
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Experimental pathology
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| Research Institution | Ehime University |
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Principal Investigator |
NOSE Masato Ehime University, プロテオ医学研究センター, 教授 (70030913)
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| Co-Investigator(Kenkyū-buntansha) |
MIYAZAKI Tatsuhiko 愛媛大学, 大学院・医学系研究科, 准教授 (80239384)
SOGA Yoshiko 愛媛大学, 医学部附属病院, 助教 (10549182)
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| Co-Investigator(Renkei-kenkyūsha) |
ONO Masao 東北大学, 大学院・医学系研究科, 教授 (20302218)
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| Project Period (FY) |
2008 – 2010
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| Keywords | MRL / lpr / MSM / Ms / 糸球体腎炎 / QTL解析 / 位置的候補遺伝子 / Cd59a / プロモーター多型 / リコンビナントコンジェニックマウス |
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| Research Abstract |
This study attempted to elucidate the polygenic genetic resistance of a wild mice-derived inbred strain MSM/Ms, having a larger gene pools than commonly used laboratory mouse strains, to glomerulonephritis in an MRL/Mp-lpr/lpr (MRL-Fas^<lpr>) lupus strain of mice and thereby to understand the steps controlling development of lupus nephritis. A genome wide scan for glomerulonephritis resistance loci in a dominant inheritance form was carried out by using MRL-Fas^<lpr> x (MRL-Fas^<lpr> x MSM/Ms) F1 backcross mice. Three dominant MSM/Ms resistance loci were mapped on chromosomes 2, 13 and 4. One of the candidate genes for the locus Agnmrl was Cd59a, encoding an glycosyl phosphatidylinositol-anchored glycoprotein inhibiting the membrane attack complex of complement. Expression of Cd59a in the glomeruli was higher in MSM/Ms than in MRL-Fas^<lpr>. Different transcriptional activity of the Cd59a promoter region was observed between the parental strains of mice. Then, the recombinant congenic strain of mice MRL-Fas^<lpr>-Cd59a^<MSM> was newly established, which was significantly decreased in severity of glomerulonephritis and also renal vasculitis.
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[Journal Article] Evaluating the role of rheumatoid factors in the development of rheumatoidarthritis in a mouse model with a newly established ELISA system.2010
Author(s)
Tanaka Y, Komori H, Mori S, Soga Y, Tsubaki T, Terada M, Miyazaki T, Fujino T, Nakamura S, Kanno H, Sawasaki T, Endo Y, Nose M.
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Journal Title
Tohoku J Exp Med. 220
Pages: 199-206
Peer Reviewed
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[Journal Article] Toll-Like receptor 3 signaling induces chronic pancreatitis through the Fas/Fas ligand-mediated cytotoxicity.2009
Author(s)
Soga Y, Komori H, Miyazaki T, Arita N, Terada M, Kamada K, Tanaka Y, Fujino T, Hiasa Y, Matsuura B, Onji M, Nose M.
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Journal Title
Tohoku J Exp Med. 217(3)
Pages: 175-184
Peer Reviewed
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