2010 Fiscal Year Final Research Report
Analysis of the roles of thioredoxin peroxidase family for adaptation to oxidative stresses in malaria parasites
| Project/Area Number |
20390118
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Parasitology (including Sanitary zoology)
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| Research Institution | Obihiro University of Agriculture and Veterinary Medicine |
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Principal Investigator |
KAWAZU Shin-Ichiro Obihiro University of Agriculture and Veterinary Medicine, 原虫病研究センター, 教授 (60312295)
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| Co-Investigator(Kenkyū-buntansha) |
KANUKA Hirotaka 帯広畜産大学, 原虫病研究センター, 教授 (50342770)
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| Co-Investigator(Renkei-kenkyūsha) |
TORII Motomi 愛媛大学, 医学部, 教授 (20164072)
ARAI Meiji 香川大学, 医学部, 准教授 (30294432)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 感染症 / マラリア / ストレス / レドックス / 遺伝子改変原虫 |
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| Research Abstract |
Phenotypes observed in the rodent malaria parasite Plasmodium berghei carrying a targeted knockout of thioredoxin peroxidase, 2-Cys peroxiredoxin gene were investigated and have the following results : (1) Defect in the gametocyte (sexual stage for transition to mosquitoes) development : 2D-PAGE analysis of the blood-stage parasite proteins suggested that the defect is attributed to the protein phosphorylation ; (2) Extension in the prepatent time in the sporozoite (infective stage to mammalian hosts from mosquitoes) infection : RT-PCR analysis of the experimental infections suggested that the knockout did not have any effect on the infectivity of the sporozoite, but it affects the development of the liver-stage (EEF) in the mice.
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