2010 Fiscal Year Final Research Report
Mechanism of neuronal death induced by oxidative stress in Parkinson's disease
| Project/Area Number |
20390172
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Hygiene
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| Research Institution | Mie University |
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Principal Investigator |
OIKAWA Shinji 三重大学, 大学院・医学系研究科, 准教授 (10277006)
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| Co-Investigator(Kenkyū-buntansha) |
YAMASHIMA Tetsumori 金沢大学, 大学院・医学系研究科, 准教授 (60135077)
MURATA Mariko 三重大学, 大学院・医学系研究科, 教授 (10171141)
KATO Takuma 三重大学, 大学院・医学系研究科, 准教授 (60224515)
CHIBA Yoichi 愛知県心身障害者コロニー発達障害研究所, 病理学部, 主任研究員 (30372113)
HIRAKU Yusuke 三重大学, 大学院・医学系研究科, 講師 (30324510)
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| Project Period (FY) |
2008 – 2010
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| Keywords | パーキンソン病 / 黒質 / 神経細胞死 / 酸化ストレス / 活性酸素種 / 酸化損傷タンパク質 / プロテオミクス解析 |
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| Research Abstract |
Parkinson's disease(PD) is the second most common, chronic age-related neurodegenerative disease. The mechanisms of cell death of dopaminergic neurons in PD have not yet been fully elucidated, but increased oxidative stress is perhaps the most important initiators or mediators of neuronal damage. In this study, to identify target proteins of reactive oxygen species(ROS) oxidation, we investigated oxidative damage of proteins in substantia nigra(SN) isolated from Japanese monkeys after transient cerebral ischemia-reperfusion. The carbonyl levels of molecular chaperones(heat shock 70k Da protein 1, T-complex protein 1 subunit alpha, etc), energy metabolism-related enzymes(mitochondrial aconitase, glutamate dehydrogenase 1, etc) and structural proteins(dihydropyrimidinase-related protein 2, etc) were increased. Therefore, oxidative damage to these proteins in SN may lead to loss of the neuroprotective function, which contributes to neuronal death. Furthermore, carbonylation of these proteins may be used as a biomarker for assessing the neuronal cell death induced by oxidative stress.
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Research Products
(38 results)
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[Journal Article] Proteomic identification of hippocampal proteins vulnerable to oxidative stress in excitotoxin-induced acute neuronal injury2011
Author(s)
Furukawa A, Kawamoto Y, Chiba Y, Takei S, Hasegawa-Ishii S, Kawamura N, Yoshikawa K, Hosokawa M, Oikawa S, Kato M, Shimada A.
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Journal Title
Neurobiol Dis
Volume: 43
Pages: 706-714
Peer Reviewed
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[Journal Article] Nitrative DNA damage and Oct3/4 expression in urinary bladder cancer with Schistosoma haematobium infection2011
Author(s)
Ma N, Thanan R, Kobayashi H, Hammam O, Wishahi M, El Leithy T, Hiraku Y, Amro el-K, Oikawa S, Ohnishi S, Murata M, Kawanishi S.
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Journal Title
Biochem Biophys Res Commun
Volume: 414
Pages: 344-349
Peer Reviewed
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[Journal Article] Suppressed phosphorylation of collapsin response mediator protein-2 in the hippocampus of HCNP precursor transgenic mice2010
Author(s)
Kanamori, T., Matsukawa, N., Kobayashi, H., Uematsu, N., Sagisaka, T., Toyoda, T., Kato, D., Oikawa, S. and Ojika, K.
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Journal Title
Brain Res
Volume: 1355
Pages: 180-188
Peer Reviewed
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[Journal Article] Proteomic analysis of aging brain in SAMP10 mouse : a model of age-related cerebral degeneration2010
Author(s)
Furukawa, A., Oikawa, S., Hasegawa-Ishii, S., Chiba, Y., Kawamura, N., Takei, S., Yoshikawa, K., Hosokawa, M., Kawanishi, S. and Shimada, A.
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Journal Title
Mech Ageing Dev
Volume: 131
Pages: 379-388
Peer Reviewed
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[Journal Article] Oxidatively generated DNA damage induced by 3-amino-5-mercapto-1, 2, 4-triazole, a metabolite of carcinogenic amitrole2010
Author(s)
Furukawa, A., Oikawa, S., Harada, K., Sugiyama, H., Hiraku, Y., Murata, M., Shimada, A. and Kawanishi, S.
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Journal Title
Mutat Res-Fund Mol M
Volume: 694
Pages: 7-12
Peer Reviewed
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[Journal Article] Urinary 8-oxo-7, 8-dihydro-2'-deoxyguanosine in patients with parasite infection and effect of antiparasitic drug in relation to cholangiocarcinogenesis2008
Author(s)
Thanan R, Murata M, Pinlaor S, Sithithaworn P, Khuntikeo N, Tangkanakul W, Hiraku Y, Oikawa S, Yongvanit P, Kawanishi S.
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Journal Title
Cancer Epidemiol Biomar
Volume: 17(3)
Pages: 518-524
Peer Reviewed
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