Treatment of hepatocellular carcinoma by the modulation of alpha-1-6 fucosyltransferase (FUT8) gene expression

2010 Fiscal Year Self-evaluation Report

• Project/Area Number: 20390205
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Niigata University
• Principal Investigator: AOYGI Yutaka Niigata University, 医歯学系, 教授 (00142266)
• Project Period (FY): 2008 – 2011
• Keywords: フコース転移酵素 / 肝細胞癌 / フコシル化AFP / FUT8 / 糖鎖

Research Abstract

α1-6フコース転移酵素(FUT8)は、肝癌の生物学的悪性度の指標とされるフコシル化AFP産生に関与しているが、その機序の分子機構は解明されていない。肝癌細胞を用いてRNAiの手法によりFUT8遺伝子発現を抑制し基礎的検討を行なうことを目的とする。

Research Products

• [Journal Article] History and recent progress in evaluation of the fucosylated alpha-fetoprotein fraction (2011)
  • Author(s): Aoyagi Y, Tamura Y, Suda T, EDITORIAL
  • Journal Title: J Gastroenterol Hepatol in press
• [Journal Article] The PDZ domain binding motif (PBM) of human T-cell leukemia virus type 1 Tax can be substituted by heterologous PBMs from viral oncoproteins during T-cell transformation. (2010)
  • Author(s): Aoyagi T, Takahashi M, Higuchi M, Oie M, Tanaka Y, Kiyono T, Aoyagi Y, Fujii M.
  • Journal Title: Virus Genes. 40(2) Pages: 193-199
• [Journal Article] Clinical Advantage of Highly Sensitive On-Chip Immunoassay for Fucosylated Fraction of Alpha-Fetoprotein in Patients with Hepatocellular Carcinoma. (2010)
  • Author(s): Tamura Y, Igarashi M, Kawai H, Suda T, Satomura S, Aoyagi Y.
  • Journal Title: Dig Dis Sci. 55(12) Pages: 3576-3583
• [Journal Article] The Japan Etiology of Liver Cirrhosis Study Group. Etiology of liver cirrhosis in Japan : a nationwide survey. (2010)
  • Author(s): Michitaka K, Nishiguchi S, Aoyagi Y, Hiasa Y, Tokumoto Y, Onji M
  • Journal Title: J Gastroenterol. 45(1) Pages: 86-94
• [Journal Article] Fucosylated fraction of alpha-fetoprotein as a predictor of prognosis in patients with hepatocellular carcinoma after curative treatment. (2010)
  • Author(s): Tamura Y, Igarashi M, Suda T, Wakai T, Shirai Y, Umemura T, Tanaka T, Kakizaki S, Takagi H, Hiasa Y, Onji M, Aoyagi Y.
  • Journal Title: Dig Dis Sci. 55(7) Pages: 2095-2101
• [Journal Article] Oxidative stress induces anti-hepatitis C virus status via the activation of extracellular signal-regulated kinase. (2009)
  • Author(s): Yano M, Ikeda M, Abe KI, Kawai Y, Kuroki M, Mori K, Dansako H, Ariumi Y, Ohkoshi S, Aoyagi Y, Kato N.
  • Journal Title: Hepatology. 50(3) Pages: 678-688
• [Journal Article] Identification of a novel motif responsible for the distinctive transforming activity of human T-cell leukemia virus (HTLV) type 1 Tax1 protein from HTLV-2 Tax2. (2009)
  • Author(s): Shoji T, Higuchi M, Kondo R, Takahashi M, Oie, M Tanaka Y, Aoyagi Y, Fujii M.
  • Journal Title: Retrovirology 6:83 Pages: doi : 10.1186/1742-4690-6-83
• [Journal Article] Serum alpha-fetoprotein levels during and after interferon therapy and the development of hepatocellular carcinoma in patients with chronic hepatitis C. (2009)
  • Author(s): Tamura Y, Yamagiwa S, Aoki Y, Kurita S, Suda T, Ohkoshi S, Nomoto M, Aoyagi Y.
  • Journal Title: Dig Dis Sci. 54(11) Pages: 2530-2537
• [Journal Article] Characterization of CD133+ parenchymal cells in the liver : histology and culture. (2009)
  • Author(s): Yoshikawa S, Zen Y, Fujii T, Sato Y, Ohta T, Aoyagi Y, Nakanuma Y
  • Journal Title: World J Gastroenterol. 15(39) Pages: 4896-4906
• [Journal Article] Rearranged NF-kappa B2 gene in an adult T-cell leukemia cell line. (2008)
  • Author(s): Isogawa M, Higuchi M, Takahashi M, Oie M, Mori N, Tanaka Y, Aoyagi Y, Fujii M.
  • Journal Title: Cancer Sci. 99(4) Pages: 792-798
• [Journal Article] Sca-1+ endothelial cells (SPECs) reside in the portal area of the liver and contribute to rapid recovery from acute liver disease. (2008)
  • Author(s): Tsuchiya A, Heike T, Baba S, Fujino H, Umeda K, Matsuda Y, Nomoto M, Ichida T, Aoyagi Y, Nakahata T.
  • Journal Title: Biochem Biophys Res Commun. 365 Pages: 595-601
• [Journal Article] Temporal treatment with interferon-b prevents hepatocellular carcinoma in hepatitis B virus-X gene transgenic mice. (2007)
  • Author(s): Yamazaki K, Suzuki K, Ohkoshi S, Yano M, Kurita S, Aoki Y, Toba K, Takamura M, Yamagiwa S, Matsuda Y, Aoyagi Y.
  • Journal Title: J Hepatol. 48 Pages: 255-265