2011 Fiscal Year Final Research Report
Induction of intermediate mesoderm and kidney progenitors from embryonic stem cells
| Project/Area Number |
20390237
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Kidney internal medicine
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| Research Institution | Kumamoto University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
TANAKA Satomi 熊本大学, 発生医学研究所, 助教 (10321944)
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| Project Period (FY) |
2008 – 2011
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| Keywords | ES細胞 / 腎臓発生 |
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| Research Abstract |
This project aims at induction of the intermediate mesoderm and metanephric mesenchyme, which contains nephron progenitors, from embryonic stem(ES) cells. We generated a knock-in mouse strain in which these two kidney precursor populations were marked with GFP. Sorted GFP-positive cells were subjected to the colony-forming assay upon Wnt4 stimulation. We revealed that embryos at E9. 5, but not at E8. 5, contained colony-forming cells. As GFP-positive cells induced from ES cells were unable to form colonies, we assume that they may correspond to the E8. 5 intermediate mesoderm. Further investigation is needed to derive nephron progenitors from ES cells.
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[Journal Article] Kif26b, a kinesin family gene, regulates adhesion of the embryonic kidney mesenchyme2010
Author(s)
Uchiyama Y, Sakaguchi M, Terabayashi T, Inenaga T, Inoue S, Kobayashi C, Oshima N, Kiyonari H, Nakagata N, Sato Y, Sekiguchi K, Miki H, Araki E, Fujimura S, Tanaka SS, and Nishinakamura R.
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Journal Title
Proc. Natl. Acad. Sci. USA
Volume: 107(20)
Pages: 9240-9245
Peer Reviewed
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