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2010 Fiscal Year Final Research Report

Establishment of a method inducing embryonic stem cells to differentiate to hematopoietic stem cells (HSC) for therapeutic HSC transplantation

Research Project

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Project/Area Number 20390291
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Pediatrics
Research InstitutionThe University of Tokyo

Principal Investigator

TSUJI Kohichiro  The University of Tokyo, 医科学研究所, 准教授 (50179991)

Co-Investigator(Kenkyū-buntansha) EBIHARA Yasuhiro  東京大学, 医科学研究所, 助教 (40302608)
Project Period (FY) 2008 – 2010
Keywordsヒト胚性幹細胞(ES細胞) / 造血幹細胞 / ストローマ細胞
Research Abstract

We challenged to establish a method inducing human embryonic stem (ES) cells to differentiate into hematopoietic stem cells without animal cells and serum for their clinical application. In brief, we first transferred human ES cells onto gelatin-coated culture dishes and cultured with 5% human platelet lysate or serum-containing media to induce their differentiation into stromal cells. Then, human ES cells were cocultured with the estabshished stromal cells under animal serum-free condition for 2 weeks to generate multipotent hematopoietic cells.Thus, we succeeded the establishment of a novel method inducing human ES cells to differentiate into multipotent hematopoietic cells without anima cells and seru,.

  • Research Products

    (41 results)

All 2011 2010 2009 2008 Other

All Journal Article (9 results) (of which Peer Reviewed: 8 results) Presentation (21 results) Book (5 results) Remarks (2 results) Patent(Industrial Property Rights) (4 results) (of which Overseas: 2 results)

  • [Journal Article] Sero-prevalence of pandemic (H1N1) 2009 influenza A virus among schoolchildren and their parents inTokyo, Japan.2011

    • Author(s)
      Iwatsuki-Horimoto K, Horimoto T, Tamura D, Kiso M, Kawakami E, Hatakeyama S, Ebihara Y, Koibuchi T, Fujii T, Takahashi K, Shimojima M, Sakai-Tagawa Y, Ito M, Sakabe S, Iwasa A, Takahashi K, Ishii T, Gorai T, Tsuji K, Iwamoto A, Kawaoka Y.
    • Journal Title

      J Clinic Microbiol (掲載確定)

    • Peer Reviewed
  • [Journal Article] Reduced dose chemotherapy for acute promyelocytic leukemia with adult Down syndrome.2011

    • Author(s)
      Tsuda M, Ebihara Y, Mochizuki S, Uchimaru K, Tojo A, Tsuji K
    • Journal Title

      Br J Haematol (掲載確定)

    • Peer Reviewed
  • [Journal Article] ヒトES細胞から血液細胞への分化誘導Cytometry Research2011

    • Author(s)
      辻浩一郎
    • Journal Title

      印刷中

    • Peer Reviewed
  • [Journal Article] A 5'untranslated region containing the IRES element in the Runx1 gene is required for angiogenesis, hematopoiesis and leukemogenesis in a knock-in mouse model.2010

    • Author(s)
      Nagamachi A, Htun PW, Ma F, Miyazaki K, Yamasaki N, Kanno M, Inaba T, Honda Z, Okuda T, Oda H, Tsuji K, Honda H H(*equally contributed)
    • Journal Title

      Dev Biol 345

      Pages: 226-236

    • Peer Reviewed
  • [Journal Article] Application of whole mouse embryo culture system on stem cell research.2009

    • Author(s)
      Kulkeaw K, Mizuochi C, Horio Y, Osumi N, Tsuji K, Sugiyama D.
    • Journal Title

      Stem Cell Rev & Rep.

      Pages: 175-180

    • Peer Reviewed
  • [Journal Article] ヒトES細胞からの血液細胞の産生2009

    • Author(s)
      辻浩一郎
    • Journal Title

      人工血液 17

      Pages: 29-36

    • Peer Reviewed
  • [Journal Article] iPS細胞からの血液誘導2009

    • Author(s)
      辻浩一郎
    • Journal Title

      臨床検査 6

      Pages: 737-743

  • [Journal Article] Cardiovascular toxicity of cryopreserved cord blood cell infusion.2008

    • Author(s)
      Konuma T, Konuma T, Ooi J, Takahashi S, Tomonari A, Tsukada N, Kobayashi T, Sato A, Kato S, Kasahara S, Ebihara Y, Nagamura-Inoue T, Tsuji K, Tojo A, Asano S.
    • Journal Title

      Bone Marrrow Transplant 41

      Pages: 861-865

    • Peer Reviewed
  • [Journal Article] Identification and clinical application of fibroblasts/myofibroblasts derived from hematopoietic stem cells.2008

    • Author(s)
      Ebihara Y.
    • Journal Title

      Japan J Pediatr Hematol. 22

      Pages: 1-6

    • Peer Reviewed
  • [Presentation] ヒトiPS細胞から血液細胞への動物血清・細胞非依存的分化誘導:自己iPS細胞由来 間葉系幹細胞との共培養2011

    • Author(s)
      海老原康博, 他
    • Organizer
      第10回日本再生医療学会総会
    • Place of Presentation
      東京:京王プラザホテル
    • Year and Date
      20110301-20110302
  • [Presentation] 15-20歳の思春期症例に対する臍帯血移植の検討2010

    • Author(s)
      海老原康博, 他
    • Organizer
      第52回日本小児血液学会総会
    • Place of Presentation
      大阪:大阪国際会議場
    • Year and Date
      20101217-20101219
  • [Presentation] Differentiation of mature eosinophils derived from human embryonic and induced pluripotent stem cells.2010

    • Author(s)
      Yang W, et al.
    • Organizer
      52nd ASH Annual Meeting
    • Place of Presentation
      Orange County Convention Center, Orlando, Florida, USA
    • Year and Date
      20101204-20101207
  • [Presentation] Hematopoiesis of human induced pluripotent stem cells derived from patients with Down syndrome.2010

    • Author(s)
      Nishihama N, et al.
    • Organizer
      52nd ASH Annual Meeting
    • Place of Presentation
      Orange County Convention Center, Orlando, Florida, USA
    • Year and Date
      20101204-20101207
  • [Presentation] The impact of steroid use as a GVHD treatment or prophylaxis within100 days after CBT.2010

    • Author(s)
      河北敏郎, et al.
    • Organizer
      第72回日本血液学会学術集会
    • Place of Presentation
      神奈川:パシフィコ横浜
    • Year and Date
      20100924-20100926
  • [Presentation] Acute promyelocytic leukemia with adult Down syndrome : a case report.2010

    • Author(s)
      Tsuda M, et al.
    • Organizer
      第72回日本血液学会学術集会
    • Place of Presentation
      神奈川:パシフィコ横浜
    • Year and Date
      20100924-20100926
  • [Presentation] Generation of mature eosinophils from human embryonic and induced pluripotent stem cells.2010

    • Author(s)
      Ma F, et al.
    • Organizer
      39th Annual Scientific Meeting of ISEH
    • Place of Presentation
      Melbourne Convention & Exhibition Centre, Melbourne Australia
    • Year and Date
      20100815-20100818
  • [Presentation] ヒトES細胞から血液細胞への分化誘導2010

    • Author(s)
      辻浩一郎
    • Organizer
      第20回日本サイトメトリー学会学術集会
    • Place of Presentation
      東京:東京慈恵会医科大学
    • Year and Date
      20100626-20100627
  • [Presentation] 慢性骨髄性白血病に対する骨髄破壊的前処置を用いた臍帯血移植2010

    • Author(s)
      河北敏郎, 他
    • Organizer
      第32回日本造血細胞移植学会
    • Place of Presentation
      静岡:アクトシティ浜松
    • Year and Date
      20100219-20100220
  • [Presentation] 再発・難治性白血病4例に対するIDA-FLAG療法の効果および有効性2009

    • Author(s)
      渡辺温子, 他
    • Organizer
      第51回日本小児血液学会
    • Place of Presentation
      千葉:東京ベイホテル東急
    • Year and Date
      20091127-20091129
  • [Presentation] Second or third myeloablative allo-SCT using cord blood for adult leukemia.2009

    • Author(s)
      Kato S, et al
    • Organizer
      第71回日本血液学会総会
    • Place of Presentation
      京都:国立京都国際会館
    • Year and Date
      20091023-20091025
  • [Presentation] Human ES cell-derived MSC maintining human ES and iPS cells under animal serum-free conditions.2009

    • Author(s)
      海老原康博, et al
    • Organizer
      第71回日本血液学会総会
    • Place of Presentation
      京都:国立京都国際会館
    • Year and Date
      20091023-20091025
  • [Presentation] Derivation of mature blood cells from human induced pluripotent stem cells.2009

    • Author(s)
      Ma F, et al
    • Organizer
      38th Annual Scientific Meeting of the International Society of Experimental Hematology
    • Place of Presentation
      Horel Divani Caravel, Athens, Greece
    • Year and Date
      20090909-20090912
  • [Presentation] Human embryonic stem (ES) cell-derived mesenchymal stem cells capable of efficiently maintaining human ES and induced pluripotent stem cells under animal serum-free conditions.2009

    • Author(s)
      海老原康博, et al
    • Organizer
      7th Annual Meeting of the International Society of Stem Cell Research
    • Place of Presentation
      Centre convencions Internacional, Barcelona, Spain
    • Year and Date
      20090708-20090711
  • [Presentation] Derivation of multipotential hematopoietic progenitors and mature blood cells from human induced pluripotent stem cells.2009

    • Author(s)
      Ma F, et al
    • Organizer
      7th Keio Stem Cell Research Symposium
    • Place of Presentation
      東京:慶応義塾大学
    • Year and Date
      20090515-20090516
  • [Presentation] 15-20歳の思春期症例に対する臍帯血移植の検討2009

    • Author(s)
      海老原康博, 他
    • Organizer
      第112回日本小児科学会学術集会
    • Place of Presentation
      奈良:奈良県文化会館
    • Year and Date
      20090417-20090419
  • [Presentation] Regenerative medicine and hematopoietic stem cells.2009

    • Author(s)
      Tsuji K.
    • Organizer
      Educational Symposium for Current Pediatric Hematology
    • Place of Presentation
      中国医科学研究院/天津血液研究所病院Tianjin, China
    • Year and Date
      2009-09-18
  • [Presentation] ヒトES細胞維持機能を有するヒトES細胞由来ストローマ細胞2009

    • Author(s)
      海老原康博, 他
    • Organizer
      第8回日本再生医療学会
    • Place of Presentation
      東京:東京国際フォーラム
    • Year and Date
      2009-03-05
  • [Presentation] 15-20歳の思春期奨励に対する臍帯血移植の検討2009

    • Author(s)
      海老原康博, 他
    • Place of Presentation
      北海道:ロイトン札幌
    • Year and Date
      2009-02-05
  • [Presentation] Myelodysplastic Syndrome (MDS) and Myeloproliferative Disease (MPD) in Children : A prospective Resistration of 222 Cases.2008

    • Author(s)
      Hirabayashi S, et al.
    • Organizer
      15th annual meeting of American Society of Hematology
    • Place of Presentation
      Moscone Convention Center, San Francisco, USA
    • Year and Date
      2008-12-07
  • [Presentation] 異種動物血清を用いないヒトembryonic stem cell(hESC)から間葉系幹細胞(MSC)への分化誘導2008

    • Author(s)
      海老原康博, 他
    • Organizer
      第70回日本血液学会
    • Place of Presentation
      京都:国立京都国際会館
    • Year and Date
      2008-10-10
  • [Book] Transworld Research Network, Kerala, Stem Cell Therapy2011

    • Author(s)
      Ma F, Yang W, Tsuji K
    • Total Pages
      51-70
  • [Book] Intech, Vienna, Embryonic stem cells2011

    • Author(s)
      Ma F, Ebihara Y, Tsuji K
    • Publisher
      (掲載確定)
  • [Book] Humana Press, New York, Lineage-specific differentiation of human embryonic and induced pluripotent stem cells methods and protocols2011

    • Author(s)
      Ma F, Gu Y, Nishihama N, Ebihara Y, Tsuji K
    • Publisher
      (掲載確定)
  • [Book] 臍帯血移植の基礎と臨床 東京2011

    • Author(s)
      辻浩一郎
    • Publisher
      医学書院(掲載確定)
  • [Book] がん患者の心に寄り添うためのサイコオンコロジーの基礎と実践2009

    • Author(s)
      海老原康博
    • Total Pages
      43-47
    • Publisher
      真興交易医書出版部小児がん
  • [Remarks] ホームページ等

  • [Remarks] 東京大学医科学研究所 幹細胞治療研究センター幹細胞プロセシング分野

    • URL

      http://stemcell-u.tokyo.org/scp/

  • [Patent(Industrial Property Rights)] Method for producing eosinophils from pluripotent stem cells2010

    • Inventor(s)
      辻浩一郎、馬峰、斎藤博久、松本健治、中畑龍俊
    • Industrial Property Rights Holder
      京都大学
    • Industrial Property Number
      特許,61/419,496
    • Filing Date
      2010-12-03
    • Overseas
  • [Patent(Industrial Property Rights)] ヒト由来の多能性幹細胞を分化させる方法2010

    • Inventor(s)
      辻浩一郎、海老原康博
    • Industrial Property Rights Holder
      東京大学
    • Industrial Property Number
      特許,特願2010-222583
    • Filing Date
      2010-09-30
  • [Patent(Industrial Property Rights)] Method for producing mast cells from pluripotent stem cells2010

    • Inventor(s)
      辻浩一郎、馬峰、中畑龍俊
    • Industrial Property Rights Holder
      京都大学
    • Industrial Property Number
      特許,PCT/JP2010/065893
    • Filing Date
      2010-09-08
  • [Patent(Industrial Property Rights)] 多能性幹細胞からの肥満細胞の製造方法2009

    • Inventor(s)
      辻浩一郎、馬峰、中畑龍俊
    • Industrial Property Rights Holder
      京都大学
    • Industrial Property Number
      特許,61/240,376
    • Filing Date
      2009-09-08
    • Overseas

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Published: 2012-01-26   Modified: 2016-04-21  

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