2010 Fiscal Year Final Research Report
Analysis of unidentified autoantigens for IEN type IgA pemphigus and anti-p200 pemphigoid
| Project/Area Number |
20390308
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Dermatology
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| Research Institution | Kurume University |
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Principal Investigator |
HASHIMOTO Takashi Kurume University, 医学部, 教授 (20129597)
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| Project Period (FY) |
2008 – 2010
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| Keywords | プロテオーム / 免疫学 / 蛋白質 / 遺伝子 / バイオテクノロジー |
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| Research Abstract |
We identified the autoantigen for anti-p200 pemphigoid as laminin gamma-1 by proteomics technique, produced recombinant proteins of laminin gamma-1, and established novel ELISA. We established new immunoprecipitation technique for IgA antibodies using protein M sepharose and cultured KU8 cells which were shown to produce plenty of autoantigen for IEN type IgA pemphigus. We identified the autoantigen as IgA secretary component by two-dimensional gel electrophoresis, immunoblotting and mass spectrometric analysis.
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Research Products
(9 results)
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[Journal Article] Anti-laminin gamma-1 pemphigoid.2009
Author(s)
Dainichi T, Kurono S, Ohyama B, Ishii N, Sanzen N, Hayashi M, Shimono C, Taniguchi Y, Koga H, Karashima T, Yasumoto S, Zillikens Z, Sekiguchi K, Hashimoto T
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Journal Title
Proc Natl Acad Sci USA. 106(8)
Pages: 2800-2805
Peer Reviewed
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[Presentation] Anti-desmocollin autoantibodies in pemphigus herpetiformis and pemphigus vegetans : IgG antibodies to desmocollin 1-3 are the key factor for their characteristic phenotypes.2010
Author(s)
Ishii N, Teye K, Hamada T, Ishikawa T, Sakaguchi S, Fukuda S, Saruta H, Karashima T, Nakama T, Yasumoto S, Hashimoto T
Organizer
The 40th annual European Society for Dermatological Research meeting
Place of Presentation
Helsinki, Finland
Year and Date
20100908-20100911
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