Radiobiological study of proton beams for optimization of therapeutic effects on brain tumors

2010 Fiscal Year Final Research Report

• Project/Area Number: 20390323
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Radiation science
• Research Institution: University of Tsukuba
• Principal Investigator: TSUBOI Koji University of Tsukuba, 大学院・人間総合科学研究科, 教授 (90188615)
• Co-Investigator(Kenkyū-buntansha): ANZAI Kazunori 日本薬科大学, 薬品物理化学分野, 教授 (70128643) ; TAKANO Shingo 筑波大学, 大学院・人間総合科学研究科, 准教授 (50292553) ; SAKAE Takeji 筑波大学, 大学院・人間総合科学研究科, 教授 (60162278) ; MORITAKE Takashi 筑波大学, 大学院・人間総合科学研究科, 講師 (50450432) ; YASUOKA Kiyoshi 筑波大学, 大学院・人間総合科学研究科, 講師 (50200499) ; TERUMUNA Toshiyuki 筑波大学, 大学院・人間総合科学研究科, 助手 (40361349)
• Project Period (FY): 2008 – 2010
• Keywords: 陽子線 / 脳腫瘍 / ラジカル / RBE / がん幹細胞

Research Abstract

In order to optimize the effects of proton beams radiotherapy for brain tumors, some uncertainties in the biological effects of proton beams were resolved in this study. First, characteristics of proton beams in DNA base damage and strand breaks were clarified compared to those of x-rays. Second, RBE values of proton beams to x-rays were measured in different endpoints. In addition, cancer-stemness in isolated radioresistant clones of brain tumor cells was demonstrated, indicating that population of cancer-stem like cells were enriched in survivors after irradiation.

Research Products

• [Journal Article] 脳腫瘍に対する陽子線治療 (2011)
  • Author(s): 坪井康次
  • Journal Title: 脳神経外科速報 vol.21no.1 Pages: 48-55
• [Journal Article] 新時代の脳腫瘍学 -診断・治療の最前線- V.脳腫瘍の治療 脳腫瘍の放射線治療陽子線治療 (2010)
  • Author(s): 水本斉志、坪井康次
  • Journal Title: 日本臨床 68巻 増刊号10
• [Journal Article] Induction of in situ DNA double-strand breaks and apoptosis by 200 MeV protons and 10 MV X-rays in human tumour cell lines. (2010)
  • Author(s): Gerelchuluun A, Hong Z, Sun L, Suzuki K, Terunuma T, Yasuoka K, Sakae T, Moritake T, Tsuboi K.
  • Journal Title: Int J Radiat Biol. [Epub ahead of print]
  • Peer Reviewed
• [Journal Article] Proton Beam Therapy for Hepatocellular Carcinoma : A Comparison of Three Treatment Protocols. (2010)
  • Author(s): Mizumoto M, Okumura T, Hashimoto T, Fukuda K, Oshiro Y, Fukumitsu N, Abei M, Kawaguchi A, Hayashi Y, Ookawa A, Hashii H, Kanemoto A, Moritake T, Tohno E, Tsuboi K, Sakae T, Sakurai H.
  • Journal Title: Int J Radiat Oncol Biol Phys. [Epub ahead of print]
  • Peer Reviewed
• [Journal Article] Proton Beam Therapy of Stage II and III Non-Small-Cell Lung Cancer. (2010)
  • Author(s): Nakayama H, Satoh H, Sugahara S, Kurishima K, Tsuboi K, Sakurai H, Ishikawa S, Tokuuye K.
  • Journal Title: Int J Radiat Oncol Biol Phys. [Epub ahead of print]
  • Peer Reviewed
• [Journal Article] Low Peripheral Lymphocyte Count Before Focal Radiotherapy Plus Concomitant Temozolomide Predicts Severe Lymphopenia During Malignant Glioma Treatment. (2010)
  • Author(s): Ishikawa E, Yamamoto T, Sakamoto N, Nakai K, Akutsu H, Tsuboi K, Takano S, Matsumura A.
  • Journal Title: Neurol Med Chir (Tokyo) 50 Pages: 638-644
  • Peer Reviewed
• [Journal Article] Clinical results of proton-beam therapy for locoregionally advanced esophageal cancer. (2010)
  • Author(s): Mizumoto M, Sugahara S, Nakayama H, Hashii H, Nakahara A, Terashima H, Okumura T, Tsuboi K, Tokuuye K, Sakurai H.
  • Journal Title: Strahlenther Onkol. 186(9) Pages: 482-488
  • Peer Reviewed
• [Journal Article] Stereotactic body radiotherapy for lung tumors at the pulmonary hilum. (2010)
  • Author(s): Oshiro Y, Aruga T, Tsuboi K, Marino K, Hara R, Sanayama Y, Itami J.
  • Journal Title: Strahlenther Onkol. 186(5) Pages: 274-279
  • Peer Reviewed
• [Journal Article] Particle radiotherapy for malignant gliomas (2009)
  • Author(s): Yamamoto T, Tsuboi K.
  • Journal Title: Brain Nerve. 61(7) Pages: 855-866
  • Peer Reviewed
• [Journal Article] Proton Beam Therapy for Large Hepatocellular Carcinoma. (2009)
  • Author(s): Sugahara S, Oshiro Y, Nakayama H, Fukuda K, Mizumoto M, Abei M, Shoda J, Matsuzaki Y, Thono E, Tokita M, Tsuboi K, Tokuuye K.
  • Journal Title: Int J Radiat Oncol Biol Phys. [Epub ahead of print]
  • Peer Reviewed
• [Journal Article] Proton beam therapy for hepatocellular carcinoma : the University of Tsukuba experience. (2009)
  • Author(s): Nakayama H, Sugahara S, Tokita M, Fukuda K, 水本斉志, Abei M, Shoda J, Sakurai H, Tsuboi K, Tokuuye K.
  • Journal Title: Cancer. 115(23) Pages: 5499-5506
  • Peer Reviewed
• [Journal Article] Technical considerations for noncoplanar proton-beam therapy of patients with tumors proximal to the optic nerve. (2009)
  • Author(s): Mizumoto M, Nakayama H, Tokita M, Sugahara S, Hashii H, Sakae T, Tsuboi K, Sakurai H, Tokuuye K.
  • Journal Title: Strahlenther Onkol. 186(1) Pages: 36-39
  • Peer Reviewed
• [Journal Article] Proton-beam therapy for hepatocellular carcinoma associated with portal vein tumor thrombosis. (2009)
  • Author(s): Sugahara S, Nakayama H, Fukuda K, Mizumoto M, Tokita M, Abei M, Shoda J, Matsuzaki Y, Thono E, Tsuboi K, Tokuuye K.
  • Journal Title: Strahlenther Onkol. 185(12) Pages: 782-788
  • Peer Reviewed
• [Journal Article] 「脳腫瘍に対する粒子線治療」Particle radiotherapies for brain tumors. (2009)
  • Author(s): 山本哲哉、坪井康次
  • Journal Title: 脳と神経
• [Journal Article] Phase I/II Trial of Hyperfractionated Concomitant Boost Proton Radiotherapy for Supratentorial Glioblastoma Multiforme. (2009)
  • Author(s): Mizumoto M, Tsuboi K, Igaki H, Yamamoto T, Takano S, Oshiro Y, Hayashi Y, Hashii H, Kanemoto A, Nakayama H, Sugahara S, Sakurai H, Matsumura A, Tokuuye K.
  • Journal Title: Int J Radiat Oncol Biol Phys. [Epub ahead of print]
  • Peer Reviewed
• [Journal Article] A prospective study of hypofractionated proton beam therapy for patients with hepatocellular carcinoma. (2009)
  • Author(s): Fukumitsu N, Sugahara S, Nakayama H, Fukuda K, Mizumoto M, Abei M, Shoda J, Thono E, Tsuboi K, Tokuuye K.
  • Journal Title: Int J Radiat Oncol Biol Phys. 74(3) Pages: 831-836
  • Peer Reviewed
• [Journal Article] Low peripheral lymphocyte count before focal radiotherapy plus concomitant temozolomide predicts severe lymphopenia during malignant glioma treatment. (2009)
  • Author(s): Eiichi Ishikawa, Tetsuya Yamamoto, Noriaki Sakamoto, Kei Nakai, Hiroyoshi Akutsu, Koji Tsuboi, Shingo Takano, Akira Matsumura.
  • Journal Title: Neurologia medico-chirurgica
  • Peer Reviewed
• [Journal Article] Dose measurement on both patients and operators during neurointerventional procedures using photoluminescence glass dosimeters. (2008)
  • Author(s): Moritake T, Matsumaru Y, Takigawa T, Nishizawa K, Matsumura A, Tsuboi K.
  • Journal Title: AJNR Am J Neuroradiol. 29(10) Pages: 1910-1917
  • Peer Reviewed
• [Journal Article] Proton beam therapy interference with implanted cardiac pacemakers. (2008)
  • Author(s): Oshiro Y, Sugahara S, Noma M, Sato M, Sakakibara Y, Sakae T, Hayashi Y, Nakayama H, Tsuboi K, Fukumitsu N, Kanemoto A, Hashimoto T, Tokuuye K.
  • Journal Title: Int J Radiat Oncol Biol Phys. 72(3) Pages: 723-727
  • Peer Reviewed
• [Presentation] Stemness of radioresistant clones solated from a medulloblastoma cell line ONS-76. (2011)
  • Author(s): 孫略
  • Organizer: The 5^<th> Taukuba Medical Science Research Meeting
  • Place of Presentation: 筑波大学臨床講義室A
  • Year and Date: 2011-01-22
• [Presentation] 高エネルギー陽子線のlineal energy値と微視的DNA損傷定量化による物理・生物学的評価システム (2010)
  • Author(s): 洪正善
  • Organizer: 日本放射線影響学会第53回大会
  • Place of Presentation: 京都テルサ
  • Year and Date: 20101020-20101022
• [Presentation] Induction of in Situ DNA Double Strand Breaks and Apoptosis by 200 MeV Protons and 10 MV X-rays in Human Tumor Cell Lines. (2010)
  • Author(s): Ariungerel Gerelchuluun
  • Organizer: 49th Annual meeting of the Particle Therapy Co-Operative Group (PTCOG49)
  • Place of Presentation: Gunma University ; National Institute of Radiological Sciences
  • Year and Date: 20100517-20100522
• [Presentation] 膠芽腫に対する高線量陽子線照射の予後 (2010)
  • Author(s): 坪井康次
  • Organizer: 第28回日本脳腫瘍学会学術集会
  • Place of Presentation: 軽井沢プリンスホテルウエスト
  • Year and Date: 2010-11-28
• [Presentation] 髄芽腫細胞株ONS76を用いた放射線耐性株クローンニングとその幹細胞様性質の解析 (2010)
  • Author(s): 孫略
  • Organizer: 日本放射線影響学会第53回大会
  • Place of Presentation: 京都テルサ
  • Year and Date: 2010-10-20
• [Presentation] 悪性脳腫瘍細胞の放射線感受性に対するCOX-2阻害剤Celecoxibの効果 (2010)
  • Author(s): 鈴木健之
  • Organizer: 日本放射線影響学会第53回大会
  • Place of Presentation: 京都テルサ
  • Year and Date: 2010-10-20
• [Presentation] 頭蓋内疾患に対する陽子線治療茨城大学研究推進プロジェクト「がん放射線治療に関する生命基礎研究」 (2010)
  • Author(s): 坪井康次
  • Organizer: 量子医療サイエンスフォーラム 公開シンポジウム(第3回Quantum Medicine 研究会)「がん放射線治療と人材養成」-基礎科学とベッドサイドを結ぶ連携を目指して-
  • Place of Presentation: 茨城大学理学部、水戸
  • Year and Date: 2010-02-06
• [Presentation] 頭蓋底腫瘍に対する陽子線過分割照射の治療成績、Hyperfraction Proton beamradiotherapy for skull base tumors in Tsukuba. (2010)
  • Author(s): 坪井康次
  • Organizer: 第23回茨城県脳腫瘍治療研究会
  • Place of Presentation: つくば国際会議場
  • Year and Date: 2010-02-05
• [Presentation] 脳腫瘍に対する放射線免疫融合療法 (2010)
  • Author(s): 坪井康次
  • Organizer: 第3回新都心神経研究会
  • Place of Presentation: 東京医科大学、新宿
  • Year and Date: 2010-01-22
• [Presentation] 陽子線と治療用X線により生成されるDNA二本鎖切断アポトーシスの定量的解析 (2009)
  • Author(s): ゲレルチュルン アリウンゲレル
  • Organizer: 第52回日本放射線影響学会
  • Place of Presentation: 広島南区民文化センター(広島市)
  • Year and Date: 20091111-20091113
• [Presentation] 高エネルギー陽子線によるDNA塩基損傷のメカニズム (2009)
  • Author(s): 洪正善
  • Organizer: 第52回日本放射線影響学会
  • Place of Presentation: 広島南区民文化センター
  • Year and Date: 20091111-20091113
• [Presentation] Prototype of real-time tumor-tracking system without implanted fiducial marker for proton therapy. (2009)
  • Author(s): T.Terunuma
  • Organizer: PTCOG48
  • Place of Presentation: Heidelberg Convention Center(ドイツ)
  • Year and Date: 20090928-20091003
• [Presentation] 陽子線と治療用X線により生成されるDNA二本鎖切断アポトーシスの定量的解析 (2009)
  • Author(s): Ariungerel Gerelchuluun
  • Organizer: 高LET放射線研究会
  • Place of Presentation: 東京大学
  • Year and Date: 20090730-20090731
• [Presentation] A phase I/II clinical trial of hyperfractionated concomitant boost proton radiotherapy for supratentorial glioblastoma multiforme. (2009)
  • Author(s): Koji Tsuboi
  • Organizer: ASTRO 2009
  • Place of Presentation: Chicago, USA Selected for "ARRO Poster Walk with a Professor"
  • Year and Date: 2009-11-03
• [Presentation] 膠芽腫における陽子線照射後のMRI変化とその病理組織学的検討 (2009)
  • Author(s): 鶴淵隆夫
  • Organizer: 第68回日本脳神経外科学会総会
  • Place of Presentation: 京王プラザホテル(東京)
  • Year and Date: 2009-10-15
• [Presentation] 脳腫瘍に対する陽子線治療 (2009)
  • Author(s): 坪井康次
  • Organizer: 第68回日本脳神経外科学会、ランチョンセミナー01:脳腫瘍と頭頚部癌の粒子線治療
  • Place of Presentation: 京王プラザホテル、東京
  • Year and Date: 2009-10-14
• [Presentation] 頭蓋底腫瘍に対する陽子線治療の長期成績 (2009)
  • Author(s): 盛武敬
  • Organizer: 第68回日本脳神経外科学会総会
  • Place of Presentation: 京王プラザホテル(東京)
  • Year and Date: 2009-10-14
• [Presentation] Hyperfractionated concomitant boost proton radiotherapy for supratentorial glioblastoma multiforme (2009)
  • Author(s): K.Tsuboi
  • Organizer: PTCOG48
  • Place of Presentation: Heidelberg Convention Center(ドイツ)
  • Year and Date: 2009-10-03
• [Presentation] 悪性脳腫瘍に対する陽子線治療 (2009)
  • Author(s): 坪井康次
  • Organizer: 第26回日本放射線腫瘍学会、シンポジウム3:脳腫瘍の放射線治療:現状と将来展望
  • Place of Presentation: 京都国際会議場
  • Year and Date: 2009-09-17
• [Presentation] Hyperfractionated concomitant boost proton radiotherapy for supratentorial glioblastoma multiforme. (2008)
  • Author(s): 坪井康次
  • Organizer: PTCOG 47.
  • Place of Presentation: Jacksonville, Florida, USA
  • Year and Date: 2008-05-23
• [Book] Glioma : Immunotherapeutic Approaches(Adoptive cell transfer therapy for malignant gliomas.)(Edited by Yamanaka R.Landes Bioscience) (2009)
  • Author(s): Eiichi Ishikawa, Shingo Takano, Tadao Ohno, Koji Tsuboi
  • Publisher: Austine, TX, USA.
• [Remarks] ホームページ等
  • URL: http://www.md.tsukuba.ac.jp/basic-med/radiation/index.html
• [Remarks]
  • URL: http://www.pmrc.tsukuba.ac.jp/