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2010 Fiscal Year Final Research Report

Analysis of cell nuclear dynamics in thermotolerance development

Research Project

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Project/Area Number 20390330
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Radiation science
Research InstitutionNara Medical University

Principal Investigator

TAKAHASHI Akihisa  Nara Medical University, 先端科学研究指導者育成ユニット, 准教授 (60275336)

Project Period (FY) 2008 – 2010
Keywords放射線治療学 / 温熱治療 / 温熱耐性 / DNA二本鎖切断 / 細胞核ダイナミクス
Research Abstract

After heat treatment at 45.5℃ for 20 min, DNA DSB recognizing proteins were partially localized with γH2AX foci. Here we show that there were slight differences in the localization pattern among these proteins, such as a disappearance from the nucleus (phospho-ATM) and translocation to the cytoplasm (phospho-NBS1) at 30 min after heat treatment, and some foci (phospho- DNA-PKcs and phospho-Chk2) appeared at 8 h after heat treatment. In addition, we demonstrated that not NHEJ deficient cells but HR deficient cells is heat-sensitivity compared with these proficient cells. Polβ functions as a critical elements involved in thermotolerance. Moreover, we indicated that heat-induced foci of γH2AX and these DNA DSB recognizing proteins were suppressed in thermotolerance development.

  • Research Products

    (71 results)

All 2011 2010 2009 2008 Other

All Journal Article (46 results) (of which Peer Reviewed: 38 results) Presentation (19 results) Book (5 results) Remarks (1 results)

  • [Journal Article] Frozen human cells can record radiation damage accumulated during space flight : mutation induction and radio-adaptation.2011

    • Author(s)
      Yatagai F, Takahashi A, et al.
    • Journal Title

      Radiat Environ Blophys. 50

      Pages: 125-134

    • Peer Reviewed
  • [Journal Article] Repair pathways independent of the Fanconi anemia nuclear core complex play a predominant role in mitigating form- aldehyde-induced DNA damage.2011

    • Author(s)
      Noda T, Takahashi A, et al.
    • Journal Title

      Blochem Biophys Res Commun. 404

      Pages: 206-210

    • Peer Reviewed
  • [Journal Article] Expression of p53-regulated genes in human cultured lymphoblastoid TSCE5 and WTK1 cell lines after spaceflight in a frozen state.2011

    • Author(s)
      高橋昭久, et al.
    • Journal Title

      Adv Space Res. 47

      Pages: 1062-1070

    • Peer Reviewed
  • [Journal Article] p53-Dependent adaptive responses in human cells exposed to space radiations.2010

    • Author(s)
      高橋昭久, et al.
    • Journal Title

      Int J Radlat Oncol Biol Phys. 78

      Pages: 1171-1176

    • Peer Reviewed
  • [Journal Article] Effect of rapamycin, an mTOR inhibitor, on radiation sensitivity of lung cancer cells having different p53 gene status.2010

    • Author(s)
      Nagata Y, Takahashi A, et al.
    • Journal Title

      Int J Oncol. 37

      Pages: 1001-1010

    • Peer Reviewed
  • [Journal Article] DNA ligase IV is a potential molecular target in ACNU sensitivity.2010

    • Author(s)
      Kondo N, Takahashi A, et al.
    • Journal Title

      Cancer Sci. 101

      Pages: 1881-1885

    • Peer Reviewed
  • [Journal Article] The expression of p53-regulated genes in human cultured lymphoblastoid TSCE5 and WTK1 cell lines during spaceflight.2010

    • Author(s)
      高橋昭久, et al.
    • Journal Title

      Int J Radiat Biol. 86

      Pages: 669-681

    • Peer Reviewed
  • [Journal Article] ATM is the predominant kinase involved in the phosphorylation of histone H2AX after heating.2010

    • Author(s)
      高橋昭久, et al.
    • Journal Title

      J Radiat Res (Tokyo). 51

      Pages: 417-422

    • Peer Reviewed
  • [Journal Article] Biphasic effects of nitric oxide radicals on radiation-induced lethality and chromosome aberrations in human lung cancer cells carrying different p53 gene status.2010

    • Author(s)
      Su X, Takahashi A, et al.
    • Journal Title

      Int J Radiat Oncol Biol Phys. 77

      Pages: 559-565

    • Peer Reviewed
  • [Journal Article] The foci of DNA double strand break-recognition proteins localize with y H2AX after heat treatment.2010

    • Author(s)
      高橋昭久, et al.
    • Journal Title

      J Radiat Res (Tokyo). 51

      Pages: 91-95

    • Peer Reviewed
  • [Journal Article] Usefulness of hexamethylenetetramine as an adjuvant to radiation and cisplatin in the treatment of solid tumors : its independency of p53 status.2010

    • Author(s)
      Masunaga S, Takahashi A, et al.
    • Journal Title

      J Radiat Res (Tokyo). 51

      Pages: 27-35

    • Peer Reviewed
  • [Journal Article] The first life science experiments in ISS : Reports of "Rad Gene"-space radiation effects on human cultured cells-.2010

    • Author(s)
      高橋昭久, et al.
    • Journal Title

      Biol Sci Space. 24

      Pages: 17-41

    • Peer Reviewed
  • [Journal Article] Recent advances in the biology of heavy-ion cancer therapy.2010

    • Author(s)
      Hamada N, Takahashi A, et al.
    • Journal Title

      J Radiat Res (Tokyo). 51

      Pages: 365-383

    • Peer Reviewed
  • [Journal Article] Effective new cancer therapies which are independent of p53 gene status.2010

    • Author(s)
      高橋昭久, et al.
    • Journal Title

      J Nara Bed Assoc. 61

      Pages: 19-33

    • Peer Reviewed
  • [Journal Article] DNA damage induced by alkylating agents and repair pathways.2010

    • Author(s)
      Kondo N, Takahashi A, et al.
    • Journal Title

      J Nucleic Acids.(Article ID 543531) 7

    • Peer Reviewed
  • [Journal Article] 国際宇宙ステーションKibo利用宇宙実験「RadGene」の成果2010

    • Author(s)
      高橋昭久,et al.
    • Journal Title

      生物工学会誌 88

      Pages: 271-279

  • [Journal Article] 国際宇宙ステーションKibo利用宇宙実験「Rad Gene」の成果報告2010

    • Author(s)
      大西武雄,高橋昭久,et al.
    • Journal Title

      放射線生物研究 45

      Pages: 103-126

  • [Journal Article] A low dose pre-irradiation induces radio-and heat-resistance via HDM2 and NO radicals, and is associated with p53 functioning.2009

    • Author(s)
      高橋昭久, et al.
    • Journal Title

      Adv Space Res. 43

      Pages: 1185-1192

    • Peer Reviewed
  • [Journal Article] Chemotherapy combined with hyperthermia improves the prognosis for non-small-cell lung cancer.2009

    • Author(s)
      Toki A, Takahashi A, et al.
    • Journal Title

      Thermal Med. 25

      Pages: 115-122

    • Peer Reviewed
  • [Journal Article] Detection of space radiation-induced double strand breaks as a track in cell nucleus.2009

    • Author(s)
      Ohnishi T, Takahashi A, et al.
    • Journal Title

      Biochem Biophys Res Commun. 390

      Pages: 485-488

    • Peer Reviewed
  • [Journal Article] DNA ligase IV as a new molecular target for temozolomide.2009

    • Author(s)
      Kondo N, Takahashi A, et al.
    • Journal Title

      Biochem Biophys Res Commun. 387

      Pages: 656-660

    • Peer Reviewed
  • [Journal Article] Restraint plus water-immersion stress (RWIS)-induced stomach ulcers in mice are depressed by a conditioning heat treatment.2009

    • Author(s)
      Yamagata R, Takahashi A, et al.
    • Journal Title

      Thermal Med. 25

      Pages: 81-87

    • Peer Reviewed
  • [Journal Article] LOH analyses for biological effects of space radiation : human cell culture in "Kibo" of International Space Station.2009

    • Author(s)
      Yatagai F, Takahashi A, et al.
    • Journal Title

      Biol Sci Space. 23

      Pages: 11-16

    • Peer Reviewed
  • [Journal Article] Expression of p53-regulated genes in cultured mammalian cells after exposure to a space environment.2009

    • Author(s)
      Ohnishi T, Takahashi A, et al.
    • Journal Title

      Biol Sci Space. 23

      Pages: 3-10

    • Peer Reviewed
  • [Journal Article] A priming heat treatment can induce the development of heat-and radio-resistance via HSPs, regardless of p53-gene status.2009

    • Author(s)
      高橋昭久, et al.
    • Journal Title

      Thermal Med. 25

      Pages: 13-23

    • Peer Reviewed
  • [Journal Article] Computational image processing and analysis of heat-induced γ H2AX foci.2009

    • Author(s)
      Somakos GI, Takahashi A, et al.
    • Journal Title

      Thermal Med. 25

      Pages: 25-34

    • Peer Reviewed
  • [Journal Article] DNA double-strand breaks : their production, recognition, and repair in eukaryotes.2009

    • Author(s)
      Ohnishi T, Takahashi A, et al.
    • Journal Title

      Mutat Res. 669

      Pages: 8-12

    • Peer Reviewed
  • [Journal Article] Molecular mechanisms involved in adaptive responses to radiation, UV light, and heat.2009

    • Author(s)
      高橋昭久, et al.
    • Journal Title

      J Radiat Res (Tokyo). 50

      Pages: 385-393

    • Peer Reviewed
  • [Journal Article] Heat-induced signal transduction pathways leading to cell death and cell survival in cancer cells.2009

    • Author(s)
      Kajihara A, Takahashi A, et al.
    • Journal Title

      Thermal Med. 25

      Pages: 1-11

    • Peer Reviewed
  • [Journal Article] High LET heavy ion radiation induces p53-independent apoptosis.2009

    • Author(s)
      Mori E, Takahashi A, et al.
    • Journal Title

      J Radlat Res (Tokyo). 50

      Pages: 37-42

    • Peer Reviewed
  • [Journal Article] 重粒子線がん治療における基礎研究2009

    • Author(s)
      高橋昭久,et al.
    • Journal Title

      放射線生物研究 44

      Pages: 208-215

  • [Journal Article] アルキル化剤によるDNA損傷修復メカニズムとそのがん治療への応用2009

    • Author(s)
      近藤夏子,高橋昭久,et al.
    • Journal Title

      放射線堂物研究 44

      Pages: 140-153

  • [Journal Article] Protein microarray analysis of apoptosis-related protein expression following heat shock in human tongue squamous cell carcinomas containing different p53 phenotypes.2008

    • Author(s)
      Kajihara A, Takahashi A, et al.
    • Journal Title

      Int J Hyperthermia. 24

      Pages: 605-612

    • Peer Reviewed
  • [Journal Article] DNA damage recognition proteins localize along heavy ion induced tracks in the cell nucleus.2008

    • Author(s)
      高橋昭久, et al.
    • Journal Title

      J Radiat Res (Tokyo). 49

      Pages: 645-652

    • Peer Reviewed
  • [Journal Article] Heat induces y H2AX foci.2008

    • Author(s)
      高橋昭久, et al.
    • Journal Title

      Mutat Res. 656

      Pages: 88-92

    • Peer Reviewed
  • [Journal Article] High LET radiation enhances apoptosis in mutated p53 cancer cells through Caspase-9 activation.2008

    • Author(s)
      Yamakawa N, Takahashi A, et al.
    • Journal Title

      Cancer Sci. 99

      Pages: 1455-1460

    • Peer Reviewed
  • [Journal Article] Effective enhancement of X-ray-induced apoptosis in human cancer cells with mutated p53 by siRNA targeting XIAP.2008

    • Author(s)
      Ohnishi K, Takahashi A, et al.
    • Journal Title

      Oncol Rep. 20

      Pages: 57-61

    • Peer Reviewed
  • [Journal Article] Hdm2 and nitric oxide radicals contribute to the p53-dependent radioadaptive response.2008

    • Author(s)
      高橋昭久, et al.
    • Journal Title

      Int JRadiat Oncol Biol Phys. 71

      Pages: 550-558

    • Peer Reviewed
  • [Journal Article] Development of thermotolerance requires interaction between polymerase a and heat shock proteins.2008

    • Author(s)
      高橋昭久, et al.
    • Journal Title

      Cancer Sci. 99

      Pages: 973-978

    • Peer Reviewed
  • [Journal Article] Time course and spacial distribution of UV effects on human skin in organ culture.2008

    • Author(s)
      Mori E, Takahashi A, et al.
    • Journal Title

      J Radiat Res (Tokyo). 49

      Pages: 269-277

    • Peer Reviewed
  • [Journal Article] 放射線・紫外線・温熱に対する適応応答-その分子機構-2008

    • Author(s)
      高橋昭久,et al.
    • Journal Title

      放射線生物研究 43

      Pages: 409-423

  • [Journal Article] きぼうでの生物実験に向けてLOH解析による宇宙放射線の生物影響の検討2008

    • Author(s)
      谷田貝文夫,高橋昭久,et al.
    • Journal Title

      JASMA 25

      Pages: 695-700

  • [Journal Article] ほ乳動物培養細胞における宇宙環境暴露後のp53調節遺伝子群の遺伝子発現2008

    • Author(s)
      大西武雄,高橋昭久,et al.
    • Journal Title

      JASMA 25

      Pages: 686-690

  • [Journal Article] 放射線適応応答のしくみの再考2008

    • Author(s)
      大西武雄,高橋昭久
    • Journal Title

      放射線生物研究 43

      Pages: 91-99

  • [Journal Article] siRNA targeted for NBS1 enhances heat sensitivity in human anaplastic thyroid carcinoma cells.

    • Author(s)
      Okamoto N, Takahashi A, et al.
    • Journal Title

      Int J Hyperthermia. (in press)

    • Peer Reviewed
  • [Journal Article] FANCD1/BRCA2 plays predominant role in the repair of DNA damage induced by ACNU or TMZ.

    • Author(s)
      Kondo N, Takahashi A, et al.
    • Journal Title

      PLoS ONE. (in press)

    • Peer Reviewed
  • [Presentation] 温熱処理後のγH2AXとDNA二本鎖切断認識タンパク質のフォーカスが局在する2010

    • Author(s)
      高橋昭久,et al.
    • Organizer
      第69回日本癌学会学術総会
    • Place of Presentation
      大阪国際会議場(大阪)
    • Year and Date
      2010-09-24
  • [Presentation] A priming heat treatment can induce the development of heat- and radio-resistance via HSPs, regardless of p53-genestatus.2010

    • Author(s)
      高橋昭久.
    • Organizer
      日本ハイパーサーミア学会第27回大会
    • Place of Presentation
      九州大学百年講堂(福岡)(日本ハイパーサーミア学会優秀論文賞受賞講演)
    • Year and Date
      2010-09-10
  • [Presentation] 超音波、温熱および放射線で誘発されるγH2AX2010

    • Author(s)
      近藤隆,高橋昭久,et al.
    • Organizer
      第49回日本医学放射線学会生物部会学術大会
    • Place of Presentation
      ホテルさっぽろ芸文館(北海道)
    • Year and Date
      2010-07-09
  • [Presentation] 温熱による細胞死の分子機構2010

    • Author(s)
      高橋昭久
    • Organizer
      第12回関西ハイパーサーミア研究会
    • Place of Presentation
      ブリーゼプラザ(大阪)(特別講演)
    • Year and Date
      2010-07-03
  • [Presentation] がん細胞の生と死の制御2009

    • Author(s)
      高橋昭久,et al.
    • Organizer
      日本放射線影響学会第52回大会
    • Place of Presentation
      放射線影響研究所(広島)
    • Year and Date
      2009-11-11
  • [Presentation] Heat-induced DNA damage and signal transduction.2009

    • Author(s)
      高橋昭久, et al.
    • Organizer
      4th Int Congr Stress Resp Biol Med
    • Place of Presentation
      ガトーキングダム札幌ホテル(札幌)シンポジウム
    • Year and Date
      2009-10-06
  • [Presentation] ATM dominantly phosphory late histone H2AX after heat treatment2009

    • Author(s)
      高橋昭久, et al.
    • Organizer
      第68回日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜(横浜)
    • Year and Date
      2009-10-01
  • [Presentation] 温熱誘導細胞死の基礎的研究2009

    • Author(s)
      高橋昭久
    • Organizer
      日本ハイパーサーミア学会第26回大会
    • Place of Presentation
      千葉大学(千葉)特別講演(日本ハイパーサーミア学会阿部賞受賞講演)
    • Year and Date
      2009-09-11
  • [Presentation] DNA-DSB recognition proteins localize along heavy-ion induced tracks in the cell nucleus.2009

    • Author(s)
      高橋昭久, et al.
    • Organizer
      Heavy Ions Ther Space Symp
    • Place of Presentation
      Cologne (Germany)
    • Year and Date
      2009-07-06
  • [Presentation] がん細胞の温熱耐性獲得を抑制するしくみの解明2009

    • Author(s)
      高橋昭久,et al.
    • Organizer
      第11回癌治療増感研究シンポジゥム
    • Place of Presentation
      猿沢荘(奈良)
    • Year and Date
      2009-02-14
  • [Presentation] 哺乳細胞における温熱誘導γH2AXフォーカス形成2008

    • Author(s)
      高橋昭久,et al.
    • Organizer
      第67回日本癌学会学術総会
    • Place of Presentation
      名古屋国際会議場(名古屋)ワークショップ
    • Year and Date
      2008-10-28
  • [Presentation] 生物学・基礎医学の立場から~温熱によるDNA損傷について~2008

    • Author(s)
      高橋昭久
    • Organizer
      日本ハイパーサーミア学会第25回大会(特別企画ICHO2008から見た世界のハイパーサーミアの現状と将来)
    • Place of Presentation
      中部大学(名古屋)特別講演
    • Year and Date
      2008-09-12
  • [Presentation] 温熱によるヒストンH2AXリン酸化機構の解明2008

    • Author(s)
      高橋昭久,et al.
    • Organizer
      日本ハイパーサーミア学会第25回大会
    • Place of Presentation
      中部大学(名古屋)
    • Year and Date
      2008-09-12
  • [Presentation] 温熱誘導DNA二本鎖切断の生物学2008

    • Author(s)
      大西武雄,高橋昭久,et al.
    • Organizer
      日本ハイパーサーミア学会第25回大会
    • Place of Presentation
      中部大学(名古屋)
    • Year and Date
      2008-09-12
  • [Presentation] 温熱と放射線のクロストーク2008

    • Author(s)
      高橋昭久,et al.
    • Organizer
      第10回関西ハイパーサーミア研究会・第19回高温度療法臨床研究会合同研究会
    • Place of Presentation
      中部大学(名古屋)
    • Year and Date
      2008-07-26
  • [Presentation] 低線量放射線前照射による温熱抵抗性の獲得2008

    • Author(s)
      高橋昭久,et al.
    • Organizer
      第47回日本医学放射線学会生物部会学術大会
    • Place of Presentation
      ホテル日航高知旭ロイヤル(高知)
    • Year and Date
      2008-06-21
  • [Presentation] Phospho-Nbsl and Mrell proteins which recognize DSBs co-localize with y H2AX in the nucleus after heat treatment.2008

    • Author(s)
      Ohnishi T, Takahashi A, et al.
    • Organizer
      Ataxia-Telangiectasia Workshop 2008
    • Place of Presentation
      Ohtsu (Japan)
    • Year and Date
      2008-04-22
  • [Presentation] The study of target genes deciding heat-sensitivity.2008

    • Author(s)
      高橋昭久, et al.
    • Organizer
      10th Int Congr Hyperthermic Oncol
    • Place of Presentation
      Munich (Germany)
    • Year and Date
      2008-04-09
  • [Presentation] Development of thermotolerance requires interaction between polymerase a and heat shock protein.2008

    • Author(s)
      Mori E, Takahashi A, et al.
    • Organizer
      10th Int Congr Hyperthermic Oncol
    • Place of Presentation
      Munich (Germany)
    • Year and Date
      2008-04-09
  • [Book] からだと光の事典(編集.太陽紫外線防御研究委員会)(宇宙ステーションと太陽粒子線)2010

    • Author(s)
      高橋昭久,et al.
    • Total Pages
      49-52
    • Publisher
      朝倉書店
  • [Book] からだと光の事典(編集.太陽紫外線防御研究委員会)(紫外線誘導シグナルトランスダクション.その発現機構とはたらき)2010

    • Author(s)
      大西武雄,高橋昭久
    • Total Pages
      74-77
    • Publisher
      朝倉書店
  • [Book] Encyclopedia of Cancer, 2nd Edition.(γH2AX.)2009

    • Author(s)
      Ohnishi T, Takahashi A, et al.
    • Total Pages
      1378-1381
    • Publisher
      Springer
  • [Book] ハイパーサーミア-がん温熱療法ガイドブック-(編集.日本ハイパーサーミア学会)(温熱による分子損傷)2008

    • Author(s)
      高橋昭久
    • Total Pages
      126-127
    • Publisher
      毎日健康サロン
  • [Book] 温熱免疫療法-ハイパーサーミソク・イムノロジー(監修.吉川敏一)(分子レベルでの温熱の作用)2008

    • Author(s)
      高橋昭久
    • Total Pages
      2-5
    • Publisher
      診断と治療社
  • [Remarks] ホームページ等

    • URL

      http://asrldu.dept.med.gunma-u.ac.jp

URL: 

Published: 2012-01-26   Modified: 2016-04-21  

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