Establishment of immune cell therapy for transplant medical application

2010 Fiscal Year Self-evaluation Report

• Project/Area Number: 20390349
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General surgery
• Research Institution: National Research Institute for Child Health and Development
• Principal Investigator: LI Xiaokang National Research Institute for Child Health and Development, 研究所, RI管理室長 (60321890)
• Project Period (FY): 2008 – 2010
• Keywords: 移植・再生医療 / 遺伝子 / 細胞・組織 / 免疫寛容 / 細胞療法 / バイオマーカー

Research Abstract

現在用いられている免疫抑制剤による移植医療は、移植を医療としての段階へ導きはしたが、半永久的な投与による副作用、精神的および経済的な負担が大きい為、新たな拒絶反応抑制方法の確立が切望されている。我々は移植医療への応用を目指して、免疫制御リンパ球(Treg細胞)、間葉系幹細胞を移植片拒絶反応抑制に利用する免疫(抑制)細胞療法の確立を本研究の目標とする。これらの細胞を用いる事により、免疫抑制剤の使用を極力減らし、安全かつ患者の生活の質(QOL)を向上させる。

Research Products

• [Journal Article] Induction of Donor-Specific Tolerance Using Superagonistic CD28 Antibody in Rat Renal Allografts : Regulatory T-Cell Expansion Before Engraftment May Be Important. (2010)
  • Author(s): Azuma H, Isaka Y, Nomi H, Inamoto T, Li X-K, Hounig T, Takabatake Y, Ichimaru N, Ibuki N, Matsumoto K, Ubai T, Katsuoka Y, Takahara S.
  • Journal Title: Transplantation 90(12) Pages: 1328-1335
  • Peer Reviewed
• [Journal Article] Prevention of graft-versus-host diseases by in vivo supCD28mAb-expanded antigen-specific nTreg cells. (2010)
  • Author(s): Kitazawa Y, Li X-K, Liu Z, Kimura H, Isaka Y, Hunig T, Takahara S.
  • Journal Title: Cell Transplant 19(6) Pages: 765-774
  • Peer Reviewed
• [Journal Article] PD1/B7-H1 interaction contribute to the spontaneous acceptance of mouse liver allograft. (2010)
  • Author(s): Morita M, Fujino M, Jiang GP, Kitazawa Y, Xie L, Azuma M, Yagita H, Nagao S, Sugioka A, Kurosawa Y, Takahara S, Fung J, Qian S, Lu L, Li X-K.
  • Journal Title: Am J Transplant 10(1) Pages: 40-46
  • Peer Reviewed
• [Journal Article] Superagonist CD28 antibody preferentially expanded Foxp3-expressing nTreg cells and prevented graft-versus-host diseases. (2009)
  • Author(s): Kitazawa Y, Fujino M, Li X-K, Xie L, Ichimaru N, Okumi M, Nonomura N, Tsujimura A, Isaka Y, Kimura H, Hunig T, Takahara S.
  • Journal Title: Cell Transplant 18(5) Pages: 627-637
  • Peer Reviewed
• [Journal Article] Prolongation of rat major histocompatibility complex-compatible cardiac allograft survival during pregnancy. (2009)
  • Author(s): Funeshima-Fuji N, Fujino M, Xie L, Kimura H, Takahara S, Ezaki T, Zhu BT, Li X-K.
  • Journal Title: J Heart Lung Transplant 28(2) Pages: 176-182
  • Peer Reviewed
• [Journal Article] Superagonistic CD28 antibody induces donor-specific tolerance in rat renal allografts. (2008)
  • Author(s): Azuma H, Isaka Y, Li X-K, Hunig T, Skamoto T, Takabatake Y, Mizui M, Kitazawa Y, Ichimaru N, Ibuki N, Inamoto T, Katsuoka Y, TakaharaS.
  • Journal Title: Am J Transplant 8(10) Pages: 2004-2014
  • Peer Reviewed
• [Journal Article] Foxp3-expressing regulatory T-cells expanded with CD28 superagonist antibody prevent rat cardiac allograft rejection. (2008)
  • Author(s): Kitazawa Y, Fujino M, Sakai T, Azumu H, Kimura H, Isaka Y, Takahara S, Hunig T, Abe R, Li X-K.
  • Journal Title: J Heart Lung Transplant 27(4) Pages: 362-371
  • Peer Reviewed
• [Presentation] A novel biomarker gene set predicts allograft rejection by multiplex reverse transcription-polymerase chain reaction. (2010)
  • Author(s): Xie L
  • Organizer: 第14回国際免疫学会
  • Place of Presentation: 神戸
  • Year and Date: 20100822-20100827
• [Presentation] The importance of the PD-1/PD-L1 co stimulatory signal for spontaneous acceptance after mouse liver allografting. (2009)
  • Author(s): Morita M
  • Organizer: 第39回日本免疫学会総会
  • Place of Presentation: 大阪
  • Year and Date: 20091202-20091204
• [Presentation] 移植免疫寛容の誘導・維持機序の解明および免疫制御細胞療法の確立 (2009)
  • Author(s): 李小康
  • Organizer: 第36回日本臨床免疫学会総会
  • Place of Presentation: 東京
  • Year and Date: 20091017-18
• [Presentation] Bone marrow mesenchymal stem cells suppress lymphocyte proliferation and prevent rat graft-versus-host disease but cloned MSCs are failed. (2009)
  • Author(s): Kitazawa Y
  • Organizer: The Okayama 2009 CTS-JSOPMB Joint Conference
  • Place of Presentation: Okayama
  • Year and Date: 20090420-20090421
• [Presentation] Superagonist CD28 antibody wcpand antigen-specific Foxp3-expressing nTreg cell to prevent graft-versus-host disease. (2008)
  • Author(s): Kitazawa Y
  • Organizer: 第38回日本移植学会総会
  • Place of Presentation: 京都
  • Year and Date: 20081201-20081203
• [Presentation] ラット骨髄由来間葉系幹細胞によるGvHD抑制効果についての検討 (2008)
  • Author(s): 北沢祐介
  • Organizer: 第44回日本移植学会総会
  • Place of Presentation: 大阪
  • Year and Date: 20080919-20080931
• [Presentation] CD28superagonistを用いた免疫寛容誘導 (2008)
  • Author(s): 李小康
  • Organizer: 第44回日本移植学会総会
  • Place of Presentation: 大阪
  • Year and Date: 20080919-20080921
• [Presentation] The requirement of the B7-H1 for spontaneous acceptance after mouse liver allografting. (2008)
  • Author(s): Morita M
  • Organizer: 22^<th> International Congress of the Transplantation Society
  • Place of Presentation: Sydney
  • Year and Date: 20080810-20080814
• [Presentation] Superagonist CD28 antibody preferentially expand Foxp3-expressing nTreg cells to prevent graft-versus-host disease. (2008)
  • Author(s): Kitazawa Y
  • Organizer: 22^<th> International Congress of the Transplantation Society
  • Place of Presentation: Sydney
  • Year and Date: 20080810-20080814