Fluorescent in vivo imaging of bone and soft tissue tumor -primary, metastasis and therapy-

2010 Fiscal Year Final Research Report

• Project/Area Number: 20390396
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Kanazawa University
• Principal Investigator: TSUCHIYA Hiroyuki Kanazawa University, 医学系, 教授 (40227434)
• Co-Investigator(Renkei-kenkyūsha): TOMITA Katsuro 金沢大学, 医学系, 教授 (00092792) ; SHIRAI Toshiharu 金沢大学, 医学系, 助教 (20397186) ; HAYASHI Katsuhiro 金沢大学, 附属病院, 助教 (80507054)
• Project Period (FY): 2008 – 2010
• Keywords: 骨・軟部腫瘍学 / 蛍光タンパク / イメージング / 転移

Research Abstract

We have investigated in vivo sarcoma fluorescent imaging. Fluorescent alkaline phosphatase antibody can label osteosarcoma. Although GFP (green) is widely used for fluorescent imaging, it has a problem of autofluorescence. Red or infrared were better observed to detect cells without autofluorescence. Real-time imaging performed in mouse lung metastasis model, the contribution of immune cells for early step of metastasis was observed. Dendritic cells for immunotherapy combined with tumor lysate of cryo-treatment were effective.

Research Products

• [Journal Article] Real-time imaging of single cancer-cell dynamics of lung metastasis. (2010)
  • Author(s): Kimura H, Hayashi K, Yamauchi K, Yamamoto N, Tsuchiya H, Tomita K, Kishimoto H, Bouvet M, Hoffman RM.
  • Journal Title: J Cell Biochem 109 Pages: 58-64
  • Peer Reviewed
• [Journal Article] Color-coded real-time cellular imaging of lung T-lymphocyte accumulation and focus formation in a mouse asthma model. (2010)
  • Author(s): Hasegawa A, Hayashi K, Kishimoto H, Yang M, Tofukuji S, Suzuki K, Nakajima H, Hoffman RM, Shirai M, Nakayama T.
  • Journal Title: J Allergy Clin Immunol 125 Pages: 461-468
  • Peer Reviewed
• [Journal Article] Targeted therapy of spinal cord glioma with a genetically modified Salmonella typhimurium. (2010)
  • Author(s): Kimura H, Zhang L., Zhao M, Hayashi K, Tsuchiya H, Tomita K, Bouvet M, Wessels J, Hoffman RM.
  • Journal Title: Cell Prolif 43 Pages: 41-48
  • Peer Reviewed
• [Journal Article] Pedicle frozen autograft reconstruction in malignant bone tumors. (2010)
  • Author(s): Tsuchiya H, et al.
  • Journal Title: J Orthop Sci 15 Pages: 340-349
  • Peer Reviewed
• [Journal Article] Cryoimmunologic antitumor effects enhanced by dendritic cells in osteosarcoma. (2010)
  • Author(s): Kawano M, Nishida H, Nakamoto Y, Tsumura H, Tsuchiya H.
  • Journal Title: Clin Orthop Relat Res 468 Pages: 1373-1383
  • Peer Reviewed
• [Journal Article] 転移リアルタイムイメージング. (2010)
  • Author(s): 林克洋.
  • Journal Title: 臨床整外 45 Pages: 1134-1138
  • Peer Reviewed
• [Journal Article] UV light killing efficacy of fluorescent protein-expressing cancer cells in vitro and in vivo. (2010)
  • Author(s): Kimura H, Lee C, Tsuchiya H, et al.
  • Journal Title: J Cell Biochem 110 Pages: 1439-1346
• [Journal Article] Long-working-distance fluorescence microscope with high-numerical-aperture objectives for variable-magnification imaging in live mice from macro-to subcellular. (2010)
  • Author(s): Kimura H, Momiyama M, Tomita K, Tsuchiya H, Hoffman RM.
  • Journal Title: J Biomed Opt 15 Pages: 066029
  • Peer Reviewed
• [Journal Article] Cancer metastasis directly eradicated by targeted therapy with a modified Salmonella typhimurium. (2009)
  • Author(s): Hayashi K, Zhao M, Yamauchi K, Yamamoto N, Tsuchiya H, Tomita K, Hoffman RM.
  • Journal Title: J Cell Biochem 106 Pages: 992-998
• [Journal Article] In vivo gene transfer between interacting human osteosarcoma cell lines is associated with acquisition of enhanced metastatic potential. (2009)
  • Author(s): Tome Y, Tsuchiya H, et al.
  • Journal Title: J Cell Biochem 108 Pages: 362-367
  • Peer Reviewed
• [Journal Article] A color-coded orthotopic nude-mouse treatment model of brain-metastatic paralyzing spinal cord cancer that induces angiogenesis and neurogenesis. (2009)
  • Author(s): Hayashi K, Yamauchi K, Yamamoto N, Tsuchiya H, Tomita K, Bouvet M, Wessels J, Hoffman RM.
  • Journal Title: Cell Prolif 42 Pages: 75-82
  • Peer Reviewed
• [Journal Article] Systemic targeting of primary bone tumor and lung metastasis of high-grade osteosarcoma in nude mice with a tumor-selective strain of Salmonella typhimurium. (2009)
  • Author(s): Hayashi K, Zhao M, Yamauchi K, Yamamoto N, Tsuchiya H, Tomita K, Kishimoto H, Bouvet M, Hoffman RM.
  • Journal Title: Cell Cycle 8 Pages: 870-875
  • Peer Reviewed
• [Journal Article] 骨・軟部腫瘍のバイオイメージング (2008)
  • Author(s): 林克洋,土屋弘行
  • Journal Title: 関節外科 27(2008) Pages: 245-251
  • Peer Reviewed
• [Presentation] Leading technology for in vivo fluorescent sarcoma imaging. (2011)
  • Author(s): Katsuhiro Hayashi, Norio Yamamoto, Toshiharu Shirai Hideji Nishida, Akihiko Takeuchi, Hiroaki Kimura, Robert M Hoffman, Akihiko Takeuchi.
  • Organizer: AAOS American Academy of Orthopaedic Surgeons
  • Place of Presentation: USA
  • Year and Date: 20110215-20110219
• [Presentation] #1 "State of Art" Lecture : Impact of caffeine-potentiated chemotherapy on musculoskeletal sarcoma treatment #2 Surgical management of skeletal metastatic tumour. (2010)
  • Author(s): 土屋弘行.
  • Organizer: 17th Hong Kong International Cancer Congress (招待講演)
  • Place of Presentation: Cheung Kung Hai Conference Centre (Hong Kong)
  • Year and Date: 20101103-20101105
• [Presentation] Anti β lintegrin antibody inhibits osteosarcoma cell lung seeding and metastasis. (2010)
  • Author(s): Kimura H, Tsuchiya H, et al.
  • Organizer: combined meeting of orthopedic research society
  • Place of Presentation: 京都国際会議場(京都府)
  • Year and Date: 20101016-20101020
• [Presentation] Cryoimmunology in bone sarcoma. (2010)
  • Author(s): 土屋弘行.
  • Organizer: ブラジル骨軟部腫瘍学会(招待講演)
  • Place of Presentation: Porto de Galinhas(Brazil)
  • Year and Date: 20100421-20100424
• [Presentation] Real-time in vivo color-coded imaging of the interaction between immunocytes and tumor cells during cancer therapy. (2010)
  • Author(s): Kimura H, Tsuchiya H, et al.
  • Organizer: American Association for Cancer Research
  • Place of Presentation: Washington Convention Center (USA)
  • Year and Date: 20100417-20100421
• [Presentation] Real-time imaging of nuclear-cytoplasmic dynamics in UV light killing of cancer cells expressing fluorescent proteins. (2010)
  • Author(s): Kimura H, Tsuchiya H, et al.
  • Organizer: American Association for Cancer Research
  • Place of Presentation: Washington Convention Center (USA)
  • Year and Date: 20100417-20100421
• [Presentation] 骨腫瘍の治療における創意と工夫. (2010)
  • Author(s): 土屋弘行.
  • Organizer: 京都運動器疾患フォーラム(招待講演)
  • Place of Presentation: 京都ホテルオークラ(京都府)
  • Year and Date: 2010-12-05
• [Presentation] 骨腫瘍に対する最近の外科的治療. (2010)
  • Author(s): 土屋弘行.
  • Organizer: 第223回大阪臨床整形外科医会研修会(招待講演)
  • Place of Presentation: ANAクラウンプラザホテル大阪(大阪府)
  • Year and Date: 2010-11-20
• [Presentation] サルモネラ変異株を応用したリンパ節転移の治療と蛍光イメージング. (2010)
  • Author(s): 林克洋、土屋弘行.
  • Organizer: 日本整形外科学会基礎学術集会
  • Place of Presentation: 京都国際会議場(京都府)
  • Year and Date: 2010-10-14
• [Presentation] 骨腫瘍の診断と治療:悪性腫瘍に対する治療. (2010)
  • Author(s): 土屋弘行.
  • Organizer: 日本小児整形外科学会第17回研修会(招待講演)
  • Place of Presentation: 大正製薬株式会社(東京都)
  • Year and Date: 2010-08-22
• [Presentation] Targeting of primary tumor and lung metastasis of high-grade osteosarcoma in nude mice with a tumor-selective strain of Salmonella typhymurium. (2010)
  • Author(s): 林克洋, 土屋弘行.
  • Organizer: 日本整形外科学会学術総会
  • Place of Presentation: 東京国際フォーラム(東京都)
  • Year and Date: 2010-05-27
• [Presentation] カフェイン併用化学療法の過去・現在・未来. (2010)
  • Author(s): 土屋弘行.
  • Organizer: ひむかセミナー(招待講演)
  • Place of Presentation: ホテルJALシティ宮崎(宮崎県)
  • Year and Date: 2010-05-15
• [Presentation] 蛍光蛋白を使用した悪性腫瘍の転移リアルタイムイメージング. (2009)
  • Author(s): 林克洋、土屋弘行.
  • Organizer: 第24回日本整形外科学会基礎学術集会
  • Place of Presentation: パシフィコ横浜(神奈川)
  • Year and Date: 20091105-20091106
• [Presentation] 蛍光蛋白を使用した癌細胞のリンパ節転移のリアルタイムイメージング. (2009)
  • Author(s): 林克洋 土屋弘行 山本憲男 白井寿治 山内健輔 ロバートホフマン 富田勝郎.
  • Organizer: 第18回日本がん転移学会学術集会・総会
  • Place of Presentation: 札幌
  • Year and Date: 20090723-20090724
• [Presentation] 蛍光蛋白を使用した癌細胞のリンパ節転移のリアルタイムイメージング. (2009)
  • Author(s): 林克洋、土屋弘行.
  • Organizer: 第42回日本整形外科学会骨軟部腫瘍学術集会
  • Place of Presentation: パシフィコ横浜(神奈川)
  • Year and Date: 20090717-20090918
• [Presentation] Dual color imaging of angiogenesis and its inhibition in bone and soft tissue sarcoma. (2009)
  • Author(s): 林克洋、土屋弘行
  • Organizer: 第52回日本整形外科学会学術総会
  • Place of Presentation: 福岡国際会議場(福岡)
  • Year and Date: 20090514-20090517
• [Presentation] Real Time Imaging of Cancer Cell Seeding to the Lung in Live Mouse. (2009)
  • Author(s): K Hayashi, H Tsuchiya, N Yamamoto, T Shirai, H Nishida, A Takeuchi, A Takeuchi, H Kimura, K Tomita, M Bouvet, RM.Hoffman.
  • Organizer: 100th American Association for Cancer Research
  • Place of Presentation: Colorado Convention Center (USA)
  • Year and Date: 20090412-20090416
• [Presentation] Biological reconstruction after tumor resection. (2009)
  • Author(s): 土屋弘行
  • Organizer: 5th International Scientific Symposium of Total Knee Arthroplasty
  • Place of Presentation: City Hall of Krakow (Poland)
  • Year and Date: 2009-10-08
• [Presentation] 骨腫瘍再建術のUp-to-Date. (2009)
  • Author(s): 土屋弘行
  • Organizer: 第83回東京医科歯科大学整形外科集談会
  • Place of Presentation: 東京医科歯科大学(東京)
  • Year and Date: 2009-07-04
• [Presentation] Biological reconstruction for tumor surgery. (2009)
  • Author(s): 土屋弘行
  • Organizer: The 50th Memorial International Orthopaedic Symposium in Kyungpook National University
  • Place of Presentation: Kyungpook National University (韓国)
  • Year and Date: 2009-05-30
• [Presentation] Biological reconstruction in lim-savingsUrgery. (2009)
  • Author(s): 土屋弘行
  • Organizer: 台北栄民総医院50周年院慶骨科医学最新進展国際検討会
  • Place of Presentation: 台北栄民総医院(台湾)
  • Year and Date: 2009-05-23
• [Presentation] Biological Reconstruction. (2009)
  • Author(s): 土屋弘行
  • Organizer: Orthopedic Seminar at South Valley University
  • Place of Presentation: South Valley University (Egypt)
  • Year and Date: 2009-04-27
• [Presentation] マイケルブベイ,ロバートホフマンTargeted therapy of bone tumor and lung metastasis in mice with atumor-selective strain of Salmonella typhymurium. (2008)
  • Author(s): 林克洋, ミンショ, 山内健輔, 山本憲男, 土屋弘行, 富田勝郎, 岸本浩行
  • Organizer: 第67回日本癌学会
  • Place of Presentation: 名古屋国際会議場(名古屋)
  • Year and Date: 2008-12-20
• [Presentation] マウス内での蛍光蛋白を使った腫瘍と血管新生のイメージング. (2008)
  • Author(s): 林克洋, 土屋弘行, 山本憲男, 白井寿治, 山内健輔, 大成一誓, 武内章彦, 河野正典, 富田勝郎
  • Organizer: 第111回中部日本整形外科災害外科学会・学術集会
  • Place of Presentation: 石川音楽堂(石川)
  • Year and Date: 2008-10-17
• [Presentation] Imaging angiogenesis in bone and soft tissue tumors in nestin-driven green fluorescent protein transgenic nude mouse. (2008)
  • Author(s): Katsuhiro Hayashi, Hiroyuki Tsuchiya, Norio Yamamoto, Toshiharu Shirai, Kensuke Yamauchi, Akihiko Takeuchi, Hiroaki Kimura, Yasunori Tome, Masanori Kawano, Robert M.Hoffman, Katsuro Tomita
  • Organizer: 7th Asia Pacific Musculoskeletal Tumor Society
  • Place of Presentation: China National Convention Center (China)
  • Year and Date: 2008-09-26
• [Presentation] 蛍光イメージングとがん治療. (2008)
  • Author(s): 林克洋, 土屋弘行, 山本憲男, 白井寿治, 山内健輔, 大成一誓, 武内章彦, 河野正典, 富田勝郎
  • Organizer: 第11回骨軟部腫瘍セミナー
  • Place of Presentation: 金沢都ホテル(石川)
  • Year and Date: 2008-09-06
• [Presentation] ロバートホフマンサルモネラ変異株を応用したリンパ節転移の治療と蛍光イメージング. (2008)
  • Author(s): 林克洋, ミンジョ, 山内健輔, 山本憲男, 土屋弘行, 富田勝郎
  • Organizer: 第17回日本がん転移学会
  • Place of Presentation: サンロイヤルホテル鹿児島(鹿児島)
  • Year and Date: 2008-07-24
• [Presentation] 蛍光蛋白を使った腫瘍と血管新生のイメージング. (2008)
  • Author(s): 林克洋, 土屋弘行, 山本憲男, 白井寿治, 山内健輔, 武内章彦, 木村浩明, 當銘保則, 河野正典, ロバートホフマン, 富田勝郎
  • Organizer: 第41回日本整形外科学会骨軟部腫瘍学術集会
  • Place of Presentation: アクトシティ浜松(静岡)
  • Year and Date: 2008-07-18
• [Presentation] Hoffman An imageable nude-mouse treatment model of metastatic paralyzing spinal-cord cancer. (2008)
  • Author(s): Katsuhiro Hayashi, Ming Zhao, Kensuke Yamauchi, Norio Yamamoto, Hiroyuki Tsuchiya, Katsuro Tomita, Hiroyuki Kishimoto, Michael Bouvet, Robert M.
  • Organizer: 99th American Association for Cancer Research
  • Place of Presentation: San Diego Convention Center (USA)
  • Year and Date: 2008-04-12
• [Presentation] Hoffman Cancer metastasis cured by specific targeting of a modified Salmonella Typhymurium. (2008)
  • Author(s): Katsuhiro Hayashi, Ming Zhao, Kensuke Yamauchi, Norio Yamamoto, Hiroyuki Tsuchiya, Katsuro Tomita, Hiroyuki Kishimoto, Michael Bouvet, Robert M.
  • Organizer: 99th American Associati for Cancer Research
  • Place of Presentation: San Diego Convention Center (USA)
  • Year and Date: 2008-04-12
• [Presentation] Hoffman Targeting of primary bone tumor and lung metastasis of high-grade osteosarcoma in nude mice with a tumor-selective strain of Salmonella typhymurium. (2008)
  • Author(s): Katsuhiro Hayashi, Ming Zhao, Kensuke Yamauchi, Norio Yamamoto, Hiroyuki Tsuchiya, Katsuro Tomita, Hiroyuki Kishimoto, Michael Bouvet, Robert M.
  • Organizer: 99th American Association for Cancer Research
  • Place of Presentation: San Diego Convention Center (USA)
  • Year and Date: 2008-04-12
• [Remarks] ホームページ
  • URL: http://web.kanazawa-u.ac.jp/~med27/kenkyu/pages/bone_group.html