IDENTIFICATION OF MOLECULAR MARKERS FOR ADVANCED PROSTATE CANCER AND CLARIFICATION OF MOLECULAR MECHANISM DURING PROGRESSION OF PROSTATE CANCER

2011 Fiscal Year Final Research Report

• Project/Area Number: 20390420
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Urology
• Research Institution: Chiba University
• Principal Investigator: ICHIKAWA Tomohiko 千葉大学, 大学院・医学研究院, 教授 (20241953)
• Project Period (FY): 2008 – 2011
• Keywords: 前立腺癌 / 遺伝子解析 / Apolipoprotein C-1 / マイクロアレイ解析 / センダイウイルスベクター / 樹状細胞療法 / マイクロRNA

Research Abstract

Using SELDI-TOF MS, we performed protein mass profiling of the sera from the patients with castration-resistant prostate cancer and identified apolipoprotein C-I (ApoC-I), which level increased with disease progression. MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression. We examined the functional significance of such miRNAs (miR-145, miR-1 and miR-133a) in prostate cancer cells and identified the novel molecular targets, purine nucleoside phosphorylase (PNP) and Fascin homolog 1 (FSCN1). We showed that lung metastases were suppressed by the use of dendritic cells (DCs) activated by replication-competent, as well as nontransmissible-type, recombinant Sendai viruses (rSeV), in both rat and mouse prostate cancer models.

Research Products

• [Journal Article] Tumour suppressors miR-1 and miR-133a target the oncogenic function of purine nucleoside phosphorylase (PNP) in prostate cancer (2012)
  • Author(s): Kojima S, Chiyomaru T, Kawakami K, Yoshino H, Enokida H, Nohata N, Fuse M, Ichikawa T, Naya Y, Nakagawa M, Seki N.
  • Journal Title: Br J Cancer Volume: 106(2) Pages: 405-413
  • DOI: DOI:10.1038/bjc.2011.462.
  • Peer Reviewed
• [Journal Article] Restoration of miR-145 expression suppresses cell proliferation, migration and invasion in prostate cancer by targeting FSCN1 (2011)
  • Author(s): Fuse M, Nohata N, Kojima S, Sakamoto S, Chiyomaru T, Kawakami K, Enokida H, Nakagawa M, Naya Y, Ichikawa T, Seki N.
  • Journal Title: Int J Oncol Volume: 38(4) Pages: 1093-1101
  • DOI: DOI:10.3892/ijo.2011.919.
  • Peer Reviewed
• [Journal Article] RIG-I helicase-independent pathway in sendai virus-activated dendritic cells is critical for preventing lung metastasis of AT6.3 prostate cancer (2010)
  • Author(s): Kato T, Ueda Y, Kinoh H, Yoneyama Y, Matsunaga A, Komaru A, Harada Y, Suzuki H, Komiya A, Shibata S, Hasegawa M, Hayashi H, Ichikawa T, Yonemitsu Y.
  • Journal Title: Neoplasia Volume: 12(11) Pages: 906-14
  • DOI: DOI:10.1593/neo.10732
  • Peer Reviewed
• [Journal Article] The isolation and identification of apolipoprotein C-I in hormone-refractory prostate cancer using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (2009)
  • Author(s): Yamamoto-Ishikawa K, Suzuki H, Nezu M, Kamiya N, Imamoto T, Komiya A, Sogawa K, Tomonaga T, Nomura F, Ichikawa T.
  • Journal Title: Asian J Androl Volume: 11(3) Pages: 299-307
  • DOI: DOI:10.1038/aja.2008.38
  • Peer Reviewed
• [Journal Article] Sustained and NK/CD4+ T cell-dependent efficient prevention of lung metastasis induced by dendritic cells harboring recombinant sendai virus (2009)
  • Author(s): Komaru A, Ueda Y, Furuya A, Tanaka S, Yoshida K, Kato T, Kinoh H, Harada Y, Suzuki H, Inoue M, Hasegawa M, Ichikawa T, Yonemitsu Y.
  • Journal Title: J Immunol Volume: 183(7) Pages: 4211-4219
  • DOI: DOI:10.4049/jimmunol.0803845
  • Peer Reviewed
• [Journal Article] Characteristic gene expression profiles of benign prostatic hypertrophy and prostate cancer (2009)
  • Author(s): Endo T, Uzawa K, Suzuki H, Tanzawa H, Ichikawa T.
  • Journal Title: Int J Oncol Volume: 35(3) Pages: 499-509
  • DOI: DOI:10.3892/ijo_00000361
  • Peer Reviewed
• [Journal Article] Molecular analysis of multifocal prostate cancer by comparative genomic hybridization (2008)
  • Author(s): Kobayashi M, Ishida H, Shindo T, Niwa S, Kino M, Kawamura K, Kamiya N, Imamoto T, Suzuki H, Hirokawa Y, Shiraishi T, Tanizawa T, Nakatani Y, Ichikawa T.
  • Journal Title: Prostate Volume: 68(16) Pages: 1715-1724
  • DOI: DOI:10.1002/pros.20832
  • Peer Reviewed
• [Presentation] Testosterone, Androgen Receptor, and Prostate Cancer (2009)
  • Author(s): Ichikawa T, et al
  • Organizer: 5th Congress of Asia Pacific Society for the Study of Aging Male
  • Place of Presentation: 大阪国際会議場(大阪府)
  • Year and Date: 2009-10-17
• [Presentation] Basic Research for Prostate Cancer (2008)
  • Author(s): Ichikawa T.
  • Organizer: Third Joint Meeting of the American Urological Association and the Japanese Urological Association International Program at the 103rd Annual Meeting of the American Urological Association
  • Place of Presentation: Orlando(米国フロリダ州)
  • Year and Date: 2008-05-18
• [Book] 前立腺癌のすべて 基礎から最新治療まで (2011)
  • Author(s): 市川智彦, 鈴木啓悦
  • Total Pages: 368
  • Publisher: メジカルビュー社
• [Remarks]
  • URL: http://www.m.chiba-u.ac.jp/class/urology/