Genome diversity associated with in montalization and establishment of endometrial cancer stem cell isolation

2010 Fiscal Year Final Research Report

• Project/Area Number: 20390435
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: Kyushu University
• Principal Investigator: WAKE Norio Kyushu University, 医学研究院・生殖病態生理学, 教授 (60091584)
• Co-Investigator(Kenkyū-buntansha): KOBAYASHI Hiroaki 九州大学, 医学研究院, 准教授 (70260700) ; FUKUSHIMA Kotaro 九州大学, 病院・講師 (40304779)
• Project Period (FY): 2008 – 2010
• Keywords: SNP / AED / ゲノム多様性 / 遺伝子発現制御 / 癌化 / 子宮体癌 / 細胞老化シグナル

Research Abstract

There is genome diversity in Ras/ERα/MDM2/p53/p21 signalosome. Genome diversity in these signalosome influences each gene function due to allelic expression difference. AED of MDM2 p1 promoter activity is defected frequently and influences MDM2 gene function in several genes are upregulated in endometrial cancer stem cells. Among these genes, antibodies are prepared against gene products expressed in all surface, establishing the efficient method to identify the cancer stem cells.

Research Products

• [Journal Article] Sentinel node detection with (99m)Tc phytate alone is satisfactory for cervical cancer patients undergoing radical hysterectomy and pelvic lymphadenectomy (2010)
  • Author(s): Ogawa S, Kobayashi H, Amada S, Yahata H, Sonoda K, Abe K, Baba S, Sasaki M, Kaku T, Wake N
  • Journal Title: Int J Clin Oncol. 15(1) Pages: 52-58
• [Journal Article] A Definitive Haplotype Map as Determined by Genotyping Duplicated Haploid Genomes Finds a Predominant Haplotype Preference at Copy-Number Variation Events (2010)
  • Author(s): Kukita Y, Yahara K, Tahira T, Higasa K, Sonoda M, Yamamoto K, Kato K, Wake N, Hayashi K
  • Journal Title: The American journal of human genetics. 86 Pages: 918-928
• [Journal Article] Endometrial cancer side-population cells show prominent migration and have a potential to differentiate into the mesenchymal cell lineage (2010)
  • Author(s): Kato K, Takao T, Kuboyama A, Tanaka Y, Ohgami T, Yamaguchi S, Adachi S, Yoneda T, Ueoka, Y, Kato Keiji, Hayashi S, Asanoma K, Wake N
  • Journal Title: The American Journal of Pathology. 176(1) Pages: 381-392
• [Journal Article] Low-dose mithramycin exerts its anticancer effect via the p53 signaling pathway and synergizes with nutlin-3 in gynecologic cancers. (2010)
  • Author(s): Ohgami T, Kato K, Kobayashi H, Sonoda K, Inoue T, Yamaguchi S, Yoneda T, Wake N
  • Journal Title: Cancer Sci. 101(6) Pages: 1387-1395
• [Journal Article] Abnormal fetal movements, micrognathia and pulmonary hypoplasia : a case report. (2010)
  • Author(s): Morokuma S, Anami A, Tsukimori K, Fukushima K, Wake N.
  • Journal Title: Abnormal fetal movements : BMC Pregnancy & Childbirth1-3
• [Journal Article] Three cases of acute fatty liver of pregnancy : Postpartum clinical course depends on interval between onset of symptoms and termination of pregnancy (2010)
  • Author(s): Aso K, Hojo S, Yumoto Y, Fukushima K, Miyazaki M, Kotoh K, Wake N.
  • Journal Title: J Matern Fetal Neonatal Med. 23(9) Pages: 1047-1049
• [Journal Article] Induction of senescence by progesterone receptor-B activation in response to cAMP in ovarian cancer cells (2009)
  • Author(s): Takahashi A, Kato K, Kuboyama A, Inoue T, Tanaka Y, Kuhara A, Kinoshita K, Takeda S, Wake N
  • Journal Title: Gynecologic oncology
• [Journal Article] Immunohistochemical characterization of mullerian mucinous borderline tumors : possible histogenetic link with serous borderline tumors and low-grade endometrioid tumors (2009)
  • Author(s): Yasunaga M, Ohishi Y, Oda Y, Misumi M, Iwasa A, Kurihara S, Nishimura I, Okuma E, Kobayashi H, Wake N, Tsuneyoshi M
  • Journal Title: Human Pathorogy. 40(7) Pages: 965-974
• [Journal Article] Homebox gene HOPX is epigenetically silenced in human uterine endometrial cancer cells by inhibiting serum response factor (2009)
  • Author(s): Yamaguchi S, Asanoma K, Takao T, Kato K, Wake N
  • Journal Title: International Journal of Cancer. 124 Pages: 2257-2588
• [Journal Article] The Level of RCAS1 Expression Is Inversely Correlated With the Number of Vitamin-Positive Stromal Cells in Epithelial Overian Cancer (2009)
  • Author(s): Sonoda K, Miyamoto S, Kobayashi H, Ogawa S, Okugawa K, Taniguchi S, Wake N
  • Journal Title: International Journal of Gynecological Cancer. 19(5) Pages: 838-843
• [Journal Article] Can we diagnose invasive cervical cancer during pregnancy as precise as in nonpregnant women?: maternal and perinatal outcome in pregnancies complicated with cervical cancers (2009)
  • Author(s): Fukushima K, Ogawa S, Tsukimori K, Kobayashi H, Wake N
  • Journal Title: Int J Gynecol Cancer. 19(8) Pages: 1439-1445
• [Journal Article] Level of reactive oxygen species induced by p21Waf1/CIP1 is critical for the determination of cell fate (2009)
  • Author(s): Inoue T, Kato K, Kato H, Asanoma K, Kuboyama A, Ueoka Y, Yamaguchi S, Ohgami T, Wake N
  • Journal Title: Cancer Science. 100(7) Pages: 1275-1283
• [Journal Article] Hobnail-like cells in serous borderline tumor do not represent concomitant incipient clear cell neoplasms (2009)
  • Author(s): Ohishi Y, Oda Y, Kurihara S, Kaku T, Yasunaga M, Nishimura I, Okuma E, Kobayashi H, Wake N, Tsuneyoshi M
  • Journal Title: Hum Pathol. 40(8) Pages: 1168-1175
• [Journal Article] Ovarian undifferentiated carcinoma resembling giant cell carcinoma of the lung (2008)
  • Author(s): Yasunaga M, Ohishi Y, Nishimura I, Tamiya S, Iwasa A, Takagi E, Inoue T, Yahata, H, Kobayashi H, Wake N, Tsuneyoshi M
  • Journal Title: Pathology International. 58(4) Pages: 244-248
• [Journal Article] CD10 immunostaining distinguishes atypical polypoid adenomyofibroma (atypical polypoid adenomyoma) from endometrial carcinoma invading the myometrium (2008)
  • Author(s): Tanaka Y, Kato K, Mibu R, Uchida S, Asanoma Ohishi Y, Kaku T, Kobayashi H, Aishima S, Umekita Y, Wake N, Tsuneyoshi M
  • Journal Title: Human Pathology. 39(10) Pages: 1446-1453
• [Journal Article] Interstitial pregnancy in a woman with congenital afibrinogenemia. (2008)
  • Author(s): Matsushita I, Uchida S, Yamaguchi A, Tanaka Y, Ogawa S, Kato K, Tsukimori K, Wake N
  • Journal Title: The journal of obstetrics and gynaecology research. 34(59) Pages: 442-453
• [Journal Article] Homeobox gene HOPX is epigenetically silenced in human uterine endometrial cancer and suppresses estrogen-stimulated proliferation of cancer cells by inhibiting serum response factor (2008)
  • Author(s): Yakaguchi S, Asanoma K, Tanaka T, Kato K, Wake N
  • Journal Title: International journal of cancer. 124(11) Pages: 2577-2588
• [Journal Article] Endometrial stromal sarcomas and related high-grade sarcomas : immunohistochemical and molecular genetic study of 31 cases (2008)
  • Author(s): Kurihara S, Oda Y, Ohishi Y, Iwasa A, Takahira T, Kaneki E, Kobayashi H, Wake N, Tsuneyoshi M
  • Journal Title: The American Journal of Surgical Pathology. 32(8) Pages: 1228-1238
• [Remarks] ホームページ等