2010 Fiscal Year Final Research Report
Analysis of Intra-cellular signaling pathway and gene network in acute lung injury as for therapeutic strategy.
| Project/Area Number |
20390459
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Emergency medicine
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| Research Institution | Yokohama City University |
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Principal Investigator |
KURAHASHI Kiyoyasu Yokohama City University, 医学部, 准教授 (50234539)
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| Co-Investigator(Kenkyū-buntansha) |
YAZAWA Takuya 横浜市立大学, 医学研究科, 准教授 (50251054)
HIRAI Toyohiro 京都大学, 医学研究科, 助教 (20359805)
BABA Yasuko 横浜市立大学, 市民総合医療センター, 助教 (80453041)
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| Co-Investigator(Renkei-kenkyūsha) |
KANEGAE Yumi 東京大学, 医科学研究所, 助教 (80251453)
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| Project Period (FY) |
2008 – 2010
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| Keywords | FasL / エンドトキシン / 肺障害 / アポトーシス / サイトカイン / トール様受容体 / keratinocyte growth factor / transforming growth factor-beta / 肺線維化 |
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| Research Abstract |
Lung fibrosis following ARDS has high morbidity and mortality. We thought that investigation on the mechanism on lung fibrosis and key molecules causing fibrosis should be of benefit to ARDS treatments. Giving bleomycin continuously using an osmotic pump implanted subcutaneously, we crated pulmonary fibrosis on rats. Eight days after the administration of bleomycin when lung fibrosis occurs, rats were given keratinocyte growth factor (KGF) expressing adenovirus vector intratracheally. The treatment group expressed attenuated pulmonary fibrosis, oxygenation impairment, and mortality. Transforming growth factor (TGF)-beta that increased in lungs after the administration of bleomycin was reduced with KGF vector administration, which suggested the importance of TNF-beta on pulmonary fibrosis. These observations suggested the lung protective effects of epithelial growth factor on ARDS.
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