2010 Fiscal Year Final Research Report

Effects of hypoxia-targeted therapies combined with chemo-radio therapy in oral squamous cell carcinoma cells

Research Project

Project/Area Number	20390480
Research Category	Grant-in-Aid for Scientific Research (B)
Allocation Type	Single-year Grants
Section	一般
Research Field	Pathobiological dentistry/Dental radiology
Research Institution	Kochi University
Principal Investigator	YAMAMOTO Tetsuya Kochi University, 教育研究部・医療学系, 教授 (00200824)
Co-Investigator(Kenkyū-buntansha)	SASABE ERI 高知大学, 教育研究部・医療学系, 助教 (40363288) SATAKE Hidetaka 高知大学, 教育研究部・医療学系, 助教 (10304685) UEDA Eisaku 高知大学, 教育研究部・医療学系, 講師 (10203431) TATEISHI Yoshihisa 高知大学, 教育研究部・医療学系, 助教 (20372732)
Project Period (FY)	2008 – 2010
Keywords	低酸素 / 口腔癌 / 一酸化窒素
Research Abstract	We investigated the effect of the nitric oxide (NO) donor on the antitumor activity of chemotherapeutic drugs to oral squamous cell carcinoma (OSC) cells. The antitumor effect of 5-FU to OSC cells was enhanced by the combination with NO donor, NOC-18 under both normoxic and hypoxic conditions. The growth rates of tumor xenografts implanted into the subcutaneous tissues of back of the nude mice were moderately attenuated by the treatment with 5-FU or nitroglycerin (NTG) alone and the rates were strongly reduced by the combination of 5-FU and NTG. The expression levels of HIF-1α protein and its target gene products (VEGF and GLUT-1) were significantly decreased by the combination with 5-FU and NTG. Furthermore, clinical effects of chemoradiotherapy in patients with OSC were enhanced by the combination with NTG. These results suggest that the combination with NO donor and chemotherapeutic drugs is considerable in the treatment of OSC.